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Literature summary extracted from
- Influence of induction conditions on the expression of carbazole dioxygenase components (CarAa, CarAc, and CarAd) from Pseudomonas stutzeri in recombinant Escherichia coli using experimental design (2011), J. Ind. Microbiol. Biotechnol., 38, 1045-1054.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.14.12.22 | expression in Escherichia coli | Stutzerimonas stutzeri |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 1.14.12.22 | additional information | expression of the catalytic subunit CarAa is enhanced by induction at a lower cell concentration and temperature and over a longer time, while the expression of subunits CarAc and CarAd is inverse. Best co-expression condition tested is cell concentration at induction of 0.5 (absorbance at 600 nm), 37°C amd 4 h | Stutzerimonas stutzeri |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.12.22 | Stutzerimonas stutzeri | - |
- |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.12.22 | carbazole + NAD(P)H + H+ + O2 | - |
Stutzerimonas stutzeri | 2'-aminobiphenyl-2,3-diol + NAD(P)+ | - |
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