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Literature summary extracted from

  • Karaman, M.; Akkemik, E.; Budak, H.; Ciftci, M.
    In vitro effects of some drugs on human erythrocyte glutathione reductase (2012), J. Enzyme Inhib. Med. Chem., 27, 18-23.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.8.1.7 gadopentetic acid non-competitive inhibition Homo sapiens
1.8.1.7 ketotifen non-competitive inhibition Homo sapiens
1.8.1.7 meloxicam non-competitive inhibition Homo sapiens
1.8.1.7 phenyramidol competitive inhibition Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.8.1.7 glutathione disulfide + NADPH + H+ Homo sapiens
-
 glutathione + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.7 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.1.7 ammonium sulfate precipitation, 2‘‘,5‘-ADP Sepharose 4B affinity column chromatography, and Sephadex G-200 gel filtration Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.8.1.7 erythrocyte
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.1.7 1.5
-
 enzyme from hemolysate, at pH 8.0 and 25°C Homo sapiens
1.8.1.7 314
-
 after 209fold purification, at pH 8.0 and 25°C Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.7 glutathione disulfide + NADPH + H+
-
 Homo sapiens glutathione + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.8.1.7 glutathione:NADP+ oxidoreductase
-
 Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.1.7 NADPH
-
 Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.8.1.7 0.0008
-
 ketotifen in 40 mM Tris-HCl buffer, pH 8.0, at 25°C Homo sapiens
1.8.1.7 0.0061
-
 meloxicam in 40 mM Tris-HCl buffer, pH 8.0, at 25°C Homo sapiens
1.8.1.7 0.434
-
 phenyramidol in 40 mM Tris-HCl buffer, pH 8.0, at 25°C Homo sapiens
1.8.1.7 28.84
-
 gadopentetic acid in 40 mM Tris-HCl buffer, pH 8.0, at 25°C Homo sapiens

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.8.1.7 0.012
-
 in 40 mM Tris-HCl buffer, pH 8.0, at 25°C Homo sapiens ketotifen
1.8.1.7 0.029
-
 in 40 mM Tris-HCl buffer, pH 8.0, at 25°C Homo sapiens meloxicam
1.8.1.7 0.99
-
 in 40 mM Tris-HCl buffer, pH 8.0, at 25°C Homo sapiens phenyramidol
1.8.1.7 138
-
 in 40 mM Tris-HCl buffer, pH 8.0, at 25°C Homo sapiens gadopentetic acid