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Literature summary extracted from

  • Ezaki, S.; Miyaoku, K.; Nishi, K.; Tanaka, T.; Fujiwara, S.; Takagi, M.; Atomi, H.; Imanaka, T.
    Gene analysis and enzymatic properties of thermostable beta-glycosidase from Pyrococcus kodakaraensis KOD1 (1999), J. Biosci. Bioeng., 88, 130-135.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.B35
-
 Thermococcus kodakarensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.B35 0.254
-
 4-nitrophenyl beta-D-mannopyranoside pH 6.5, 80°C Thermococcus kodakarensis
3.2.1.B35 1.79
-
 4-nitrophenyl beta-D-glucopyranoside pH 6.5, 80°C Thermococcus kodakarensis
3.2.1.B35 8.37
-
 4-nitrophenyl beta-D-mannopyranoside pH 6.5, 80°C Thermococcus kodakarensis
3.2.1.B35 34.6
-
 4-nitrophenyl beta-D-galactopyranoside pH 6.5, 80°C Thermococcus kodakarensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.B35 56000
-
 x * 56000, SDS-PAGE Thermococcus kodakarensis

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.B35 Thermococcus kodakarensis Q9YGB8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.B35
-
 Thermococcus kodakarensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.B35 4-nitrophenyl beta-D-galactopyranoside + H2O
-
 Thermococcus kodakarensis 4-nitrophenol + beta-D-galactopyranose
-
 ?
3.2.1.B35 4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Thermococcus kodakarensis 4-nitrophenol + D-glucopyranose
-
 ?
3.2.1.B35 4-nitrophenyl beta-D-mannopyranoside + H2O
-
 Thermococcus kodakarensis 4-nitrophenol + beta-D-mannopyranose
-
 ?
3.2.1.B35 4-nitrophenyl beta-D-xylopyranoside + H2O
-
 Thermococcus kodakarensis 4-nitrophenol + beta-D-xylopyranose
-
 ?
3.2.1.B35 cellobiose + H2O
-
 Thermococcus kodakarensis 2 D-glucose
-
 ?
3.2.1.B35 cellopentaose + 4 H2O
-
 Thermococcus kodakarensis 5 D-glucose
-
 ?
3.2.1.B35 cellotetraose + 3 H2O
-
 Thermococcus kodakarensis 4 D-glucose
-
 ?
3.2.1.B35 cellotriose + 2 H2O
-
 Thermococcus kodakarensis 3 D-glucose
-
 ?
3.2.1.B35 additional information exo-type beta-glycosidase. The enzyme also harbors transglycosylation activity Thermococcus kodakarensis ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.B35 ? x * 56000, SDS-PAGE Thermococcus kodakarensis

Synonyms

EC Number Synonyms Comment Organism
3.2.1.B35 Pyrococcus kodakaraensis beta-glycosidase
-
 Thermococcus kodakarensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.B35 75
-
 assay at Thermococcus kodakarensis
3.2.1.B35 100
-
-
 Thermococcus kodakarensis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.B35 90
-
 half-life: more than 22 h Thermococcus kodakarensis
3.2.1.B35 100
-
 half-life: less than 1 h Thermococcus kodakarensis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.B35 186
-
 4-nitrophenyl beta-D-mannopyranoside pH 6.5, 80°C Thermococcus kodakarensis
3.2.1.B35 243
-
 4-nitrophenyl beta-D-mannopyranoside pH 6.5, 80°C Thermococcus kodakarensis
3.2.1.B35 1650
-
 4-nitrophenyl beta-D-glucopyranoside pH 6.5, 80°C Thermococcus kodakarensis
3.2.1.B35 3270
-
 4-nitrophenyl beta-D-galactopyranoside pH 6.5, 80°C Thermococcus kodakarensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.B35 6.5
-
-
 Thermococcus kodakarensis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.B35 22
-
 4-nitrophenyl beta-D-mannopyranoside pH 6.5, 80°C Thermococcus kodakarensis
3.2.1.B35 94
-
 4-nitrophenyl beta-D-galactopyranoside pH 6.5, 80°C Thermococcus kodakarensis
3.2.1.B35 922
-
 4-nitrophenyl beta-D-glucopyranoside pH 6.5, 80°C Thermococcus kodakarensis
3.2.1.B35 957
-
 4-nitrophenyl beta-D-mannopyranoside pH 6.5, 80°C Thermococcus kodakarensis