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Literature summary extracted from

  • Nitisakulkan, T.; Oku, S.; Kudo, D.; Nakashimada, Y.; Tajima, T.; Vangnai, A.; Kato, J.
    Degradation of chloroanilines by toluene dioxygenase from Pseudomonas putida T57 (2013), J. Biosci. Bioeng., 117, 292-297.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.12.11 expression of genes encoding toluene dioxygenase complex todC1C2BA in Escherichia coli Pseudomonas putida

Protein Variants

EC Number Protein Variants Comment Organism
1.14.12.11 additional information introduction of a plasmid containing the entire tod operon todC1C2BADE to Pseudomonas putida T57 to enhance its ability to degrade 4-chloroaniline. The resulting strain shows 250-fold higher 4-chloroaniline degradation activity the parental strain. The recobinant strain degrades 2-chloroaniline, 3-chloroaniline, and 3,4-dichloroaniline as well as 3-chloroaniline, but not 3,5-dichloroaniline Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
1.14.12.11 Pseudomonas putida
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1.14.12.11 Pseudomonas putida T57
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.12.11 2-chloroaniline + NADH + H+ + O2 degradation rates in decreasing order: 4-chloroaniline, 3-chloroaniline, 2-chloroaniline, 3,4-dichloroaniline Pseudomonas putida ? + NAD+
-
 ?
1.14.12.11 2-chloroaniline + NADH + H+ + O2 degradation rates in decreasing order: 4-chloroaniline, 3-chloroaniline, 2-chloroaniline, 3,4-dichloroaniline Pseudomonas putida T57 ? + NAD+
-
 ?
1.14.12.11 3,4-dichloroaniline + NADH + H+ + O2 degradation rates in decreasing order: 4-chloroaniline, 3-chloroaniline, 2-chloroaniline, 3,4-dichloroaniline Pseudomonas putida ? + NAD+
-
 ?
1.14.12.11 3,4-dichloroaniline + NADH + H+ + O2 degradation rates in decreasing order: 4-chloroaniline, 3-chloroaniline, 2-chloroaniline, 3,4-dichloroaniline Pseudomonas putida T57 ? + NAD+
-
 ?
1.14.12.11 3-chloroaniline + NADH + H+ + O2 degradation rates in decreasing order: 4-chloroaniline, 3-chloroaniline, 2-chloroaniline, 3,4-dichloroaniline Pseudomonas putida ? + NAD+
-
 ?
1.14.12.11 3-chloroaniline + NADH + H+ + O2 degradation rates in decreasing order: 4-chloroaniline, 3-chloroaniline, 2-chloroaniline, 3,4-dichloroaniline Pseudomonas putida T57 ? + NAD+
-
 ?
1.14.12.11 4-chloroaniline + NADH + H+ + O2 degradation rates in decreasing order: 4-chloroaniline, 3-chloroaniline, 2-chloroaniline, 3,4-dichloroaniline Pseudomonas putida 4-chlorocatechol + 2-amino-5-chlorophenol + NAD+ toluene dioxygenase catalyzes both 1,2- and 2,3-dioxygenation of 4-chloroaniline ?
1.14.12.11 4-chloroaniline + NADH + H+ + O2 degradation rates in decreasing order: 4-chloroaniline, 3-chloroaniline, 2-chloroaniline, 3,4-dichloroaniline Pseudomonas putida T57 4-chlorocatechol + 2-amino-5-chlorophenol + NAD+ toluene dioxygenase catalyzes both 1,2- and 2,3-dioxygenation of 4-chloroaniline ?
1.14.12.11 additional information no substrate: 3,5-dichloroaniline Pseudomonas putida ?
-
 ?
1.14.12.11 additional information no substrate: 3,5-dichloroaniline Pseudomonas putida T57 ?
-
 ?

General Information

EC Number General Information Comment Organism
1.14.12.11 physiological function expression of genes encoding toluene dioxygenase complex todC1C2BA in Escherichia coli results in the ability to degrade 4-chloroaniline Pseudomonas putida