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Literature summary extracted from

  • Nisar, M.A.; Rashid, N.; Bashir, Q.; Gardner, Q.T.; Shafiq, M.H.; Akhtar, M.
    TK1299, a highly thermostable NAD(P)H oxidase from Thermococcus kodakaraensis exhibiting higher enzymatic activity with NADPH (2013), J. Biosci. Bioeng., 116, 39-44.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.6.3.2 expression in Escherichia coli Thermococcus kodakarensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.6.3.2 0.24
-
 NADH pH 7.8, 75°C Thermococcus kodakarensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.6.3.2 48610
-
 6 * 48610, sequence calculation Thermococcus kodakarensis
1.6.3.2 49375
-
 6 * 49375, mass spectrometry Thermococcus kodakarensis
1.6.3.2 300000
-
 gel filtration Thermococcus kodakarensis

Organism

EC Number Organism UniProt Comment Textmining
1.6.3.2 Thermococcus kodakarensis Q5JGP4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.6.3.2
-
 Thermococcus kodakarensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.3.2 3 NADH + 3 H+ + 2 O2 approximately 75% H2O and 25% H2O is produced. Km value of the enzyme towards NADH and NADPH is almost the same whereas specific activity is higher with NADPH compared to NADH Thermococcus kodakarensis 3 NAD+ + H2O
-
 ?
1.6.3.2 3 NADPH + 3 H+ + 2 O2 approximately 87% H2O and 13% H2O2 is produced. Km value of the enzyme towards NADH and NADPH is almost the same whereas specific activity is higher with NADPH compared to NADH Thermococcus kodakarensis 3 NADP+ + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.6.3.2 hexamer 6 * 48610, sequence calculation Thermococcus kodakarensis
1.6.3.2 hexamer 6 * 49375, mass spectrometry Thermococcus kodakarensis

Synonyms

EC Number Synonyms Comment Organism
1.6.3.2 KOD1
-
 Thermococcus kodakarensis
1.6.3.2 NAD(P)H oxidase
-
 Thermococcus kodakarensis
1.6.3.2 TK1299
-
 Thermococcus kodakarensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.6.3.2 75
-
-
 Thermococcus kodakarensis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.6.3.2 70 80 70°C: about 60% of maximal activity, 80°C: about 70% of maximal activity Thermococcus kodakarensis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.6.3.2 100
-
 150 min, less than 5% loss of activity Thermococcus kodakarensis
1.6.3.2 120
-
 secondary structure of the enzyme is not stable at this temperature Thermococcus kodakarensis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.6.3.2 68
-
 NADH pH 7.8, 75°C Thermococcus kodakarensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.6.3.2 8
-
-
 Thermococcus kodakarensis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.6.3.2 7.5 8.5 pH 7.5: about 70% of maximal activity, pH 8.5: about 70% of maximal activity Thermococcus kodakarensis

Cofactor

EC Number Cofactor Comment Organism Structure
1.6.3.2 FAD tightly bound, 0.5 FAD attached per subunit Thermococcus kodakarensis