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Literature summary extracted from

  • Kabashima, Y.; Ueda, N.; Sone, N.; Sakamoto, J.
    Mutation analysis of the interaction of B-type cytochrome c oxidase with its natural substrate cytochrome c-551 (2010), J. Biosci. Bioeng., 109, 325-330.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
7.1.1.9 D49N the mutant shows increased Km and reduced kcat values compared to the wild type enzyme Geobacillus thermodenitrificans
7.1.1.9 D99N the mutant shows strongly increased Km and reduced kcat values compared to the wild type enzyme Geobacillus thermodenitrificans
7.1.1.9 E116Q the mutant shows slightly reduced Km and strongly reduced kcat values compared to the wild type enzyme Geobacillus thermodenitrificans
7.1.1.9 E139Q the mutant shows increased Km and slightly increased kcat values compared to the wild type enzyme Geobacillus thermodenitrificans
7.1.1.9 E64Q the mutant shows increased Km and kcat values compared to the wild type enzyme Geobacillus thermodenitrificans
7.1.1.9 E66Q the mutant shows increased Km and kcat values compared to the wild type enzyme Geobacillus thermodenitrificans
7.1.1.9 E68Q the mutant shows increased Km and reduced kcat values compared to the wild type enzyme Geobacillus thermodenitrificans
7.1.1.9 E84Q the mutant shows increased Km and reduced kcat values compared to the wild type enzyme Geobacillus thermodenitrificans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
7.1.1.9 0.000023
-
 reduced cytochrome c551 mutant enzyme E116Q, at 40°C, 200 mM KCl, 1 mM MgSO4, 1 mM sodium phosphate buffer, pH 6.8 Geobacillus thermodenitrificans
7.1.1.9 0.000026
-
 reduced cytochrome c551 wild type enzyme, at 40°C, 200 mM KCl, 1 mM MgSO4, 1 mM sodium phosphate buffer, pH 6.8 Geobacillus thermodenitrificans
7.1.1.9 0.000035
-
 reduced cytochrome c551 mutant enzyme E84Q, at 40°C, 200 mM KCl, 1 mM MgSO4, 1 mM sodium phosphate buffer, pH 6.8 Geobacillus thermodenitrificans
7.1.1.9 0.000044
-
 reduced cytochrome c551 mutant enzyme D49N, at 40°C, 200 mM KCl, 1 mM MgSO4, 1 mM sodium phosphate buffer, pH 6.8 Geobacillus thermodenitrificans
7.1.1.9 0.000059
-
 reduced cytochrome c551 mutant enzyme E66Q, at 40°C, 200 mM KCl, 1 mM MgSO4, 1 mM sodium phosphate buffer, pH 6.8 Geobacillus thermodenitrificans
7.1.1.9 0.000063
-
 reduced cytochrome c551 mutant enzyme E139Q, at 40°C, 200 mM KCl, 1 mM MgSO4, 1 mM sodium phosphate buffer, pH 6.8 Geobacillus thermodenitrificans
7.1.1.9 0.000074
-
 reduced cytochrome c551 mutant enzyme E68Q, at 40°C, 200 mM KCl, 1 mM MgSO4, 1 mM sodium phosphate buffer, pH 6.8 Geobacillus thermodenitrificans
7.1.1.9 0.0001
-
 reduced cytochrome c551 mutant enzyme E64Q, at 40°C, 200 mM KCl, 1 mM MgSO4, 1 mM sodium phosphate buffer, pH 6.8 Geobacillus thermodenitrificans
7.1.1.9 0.000154
-
 reduced cytochrome c551 mutant enzyme D99N, at 40°C, 200 mM KCl, 1 mM MgSO4, 1 mM sodium phosphate buffer, pH 6.8 Geobacillus thermodenitrificans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
7.1.1.9 membrane
-
 Geobacillus thermodenitrificans 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
7.1.1.9 5976
-
 1 * 60371 + 1 * 17074 + 1 * 5976, calculated from amino acid sequence Geobacillus thermodenitrificans
7.1.1.9 17074
-
 1 * 60371 + 1 * 17074 + 1 * 5976, calculated from amino acid sequence Geobacillus thermodenitrificans
7.1.1.9 60371
-
 1 * 60371 + 1 * 17074 + 1 * 5976, calculated from amino acid sequence Geobacillus thermodenitrificans

Organism

EC Number Organism UniProt Comment Textmining
7.1.1.9 Geobacillus thermodenitrificans
-
 formerly Bacillus stearothermophilus
-
7.1.1.9 Geobacillus thermodenitrificans K1041
-
 formerly Bacillus stearothermophilus
-

Purification (Commentary)

EC Number Purification (Comment) Organism
7.1.1.9 DEAE-Toyopearl column chromatography and hydroxyapatite column chromatography Geobacillus thermodenitrificans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.1.1.9 reduced cytochrome c551 + O2 + H+
-
 Geobacillus thermodenitrificans oxidized cytochrome c551 + H2O
-
 ?
7.1.1.9 reduced cytochrome c551 + O2 + H+
-
 Geobacillus thermodenitrificans K1041 oxidized cytochrome c551 + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
7.1.1.9 heterotrimer 1 * 60371 + 1 * 17074 + 1 * 5976, calculated from amino acid sequence Geobacillus thermodenitrificans

Synonyms

EC Number Synonyms Comment Organism
7.1.1.9 cytochrome bo3 oxidase
-
 Geobacillus thermodenitrificans
7.1.1.9 cytochrome bo3-type cytochrome c oxidase
-
 Geobacillus thermodenitrificans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
7.1.1.9 30
-
 reduced cytochrome c551 mutant enzyme E116Q, at 40°C, 200 mM KCl, 1 mM MgSO4, 1 mM sodium phosphate buffer, pH 6.8 Geobacillus thermodenitrificans
7.1.1.9 140
-
 reduced cytochrome c551 mutant enzyme D99N, at 40°C, 200 mM KCl, 1 mM MgSO4, 1 mM sodium phosphate buffer, pH 6.8 Geobacillus thermodenitrificans
7.1.1.9 199
-
 reduced cytochrome c551 mutant enzyme E66Q, at 40°C, 200 mM KCl, 1 mM MgSO4, 1 mM sodium phosphate buffer, pH 6.8 Geobacillus thermodenitrificans
7.1.1.9 200
-
 reduced cytochrome c551 mutant enzyme D49N, at 40°C, 200 mM KCl, 1 mM MgSO4, 1 mM sodium phosphate buffer, pH 6.8 Geobacillus thermodenitrificans
7.1.1.9 290
-
 reduced cytochrome c551 mutant enzyme E68Q, at 40°C, 200 mM KCl, 1 mM MgSO4, 1 mM sodium phosphate buffer, pH 6.8 Geobacillus thermodenitrificans
7.1.1.9 295
-
 reduced cytochrome c551 wild type enzyme, at 40°C, 200 mM KCl, 1 mM MgSO4, 1 mM sodium phosphate buffer, pH 6.8 Geobacillus thermodenitrificans
7.1.1.9 297
-
 reduced cytochrome c551 mutant enzyme E139Q, at 40°C, 200 mM KCl, 1 mM MgSO4, 1 mM sodium phosphate buffer, pH 6.8 Geobacillus thermodenitrificans
7.1.1.9 317
-
 reduced cytochrome c551 mutant enzyme E64Q, at 40°C, 200 mM KCl, 1 mM MgSO4, 1 mM sodium phosphate buffer, pH 6.8 Geobacillus thermodenitrificans
7.1.1.9 325
-
 reduced cytochrome c551 mutant enzyme E84Q, at 40°C, 200 mM KCl, 1 mM MgSO4, 1 mM sodium phosphate buffer, pH 6.8 Geobacillus thermodenitrificans

Cofactor

EC Number Cofactor Comment Organism Structure
7.1.1.9 heme
-
 Geobacillus thermodenitrificans