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Literature summary extracted from

  • Koryakina, I.; Neville, J.; Nonaka, K.; van Lanen, S.G.; Williams, G.J.
    A high-throughput screen for directed evolution of the natural product sulfotransferase LipB (2011), J. Biomol. Screen., 16, 845-851.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.8.2.B5 expression in Escherichia coli Streptomyces sp.

Protein Variants

EC Number Protein Variants Comment Organism
2.8.2.B5 additional information colorimetric, high-throughput assay suitable for optimizing the activity of the recently discovered sulfotransferase LipB, by directed evolution. Mutant LipBs with significantly improved activity are not identified Streptomyces sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.2.B5 additional information Streptomyces sp. sulfation is used as a self-resistance strategy that leads to the in vivo inactivation of liposidomycins as well as that of the A-90289s ?
-
 ?
2.8.2.B5 additional information Streptomyces sp. SANK 60405 sulfation is used as a self-resistance strategy that leads to the in vivo inactivation of liposidomycins as well as that of the A-90289s ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.8.2.B5 Streptomyces sp.
-
-
-
2.8.2.B5 Streptomyces sp. SANK 60405
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.2.B5
-
 Streptomyces sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.2.B5 4-nitrophenyl sulfate + desulfo-A-90289A
-
 Streptomyces sp. 4-nitrophenol + A-90289A
-
 ?
2.8.2.B5 4-nitrophenyl sulfate + desulfo-A-90289A
-
 Streptomyces sp. SANK 60405 4-nitrophenol + A-90289A
-
 ?
2.8.2.B5 4-nitrophenyl sulfate + uridine
-
 Streptomyces sp. 4-nitrophenol + uridine 2'-sulfate
-
 ?
2.8.2.B5 4-nitrophenyl sulfate + uridine
-
 Streptomyces sp. SANK 60405 4-nitrophenol + uridine 2'-sulfate
-
 ?
2.8.2.B5 additional information sulfation is used as a self-resistance strategy that leads to the in vivo inactivation of liposidomycins as well as that of the A-90289s Streptomyces sp. ?
-
 ?
2.8.2.B5 additional information sulfation is used as a self-resistance strategy that leads to the in vivo inactivation of liposidomycins as well as that of the A-90289s Streptomyces sp. SANK 60405 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.8.2.B5 LipB ambiguous Streptomyces sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.8.2.B5 25
-
 assay at Streptomyces sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.8.2.B5 8
-
 assay at Streptomyces sp.