Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Humnabadkar, V.; Jha, R.K.; Ghatnekar, N.; De Sousa, S.M.
    A high-throughput screening assay for simultaneous selection of inhibitors of Mycobacterium tuberculosis 1-deoxy-D-xylulose-5-phosphate synthase (Dxs) or 1-deoxy-D-xylulose 5-phosphate reductoisomerase (Dxr) (2011), J. Biomol. Screen., 16, 303-312.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.267 gene Rv2870c, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Mycobacterium tuberculosis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.267 fosmidomycin
-
Mycobacterium tuberculosis
1.1.1.267 additional information development and evaluation of a high-throughput screening spectrometric assay, measuring Dxr activity of Dxr coupled with 1-deoxy-D-xylulose-5-phosphate synthase, Dxs, activity, for simultaneous selection of inhibitors of the enzyme, overview Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.267 0.01
-
NADPH pH 7.5, 25°C, recombinant enzyme Mycobacterium tuberculosis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.267 Co2+ can partially substitute for Mg2+ or Mn2+ Mycobacterium tuberculosis
1.1.1.267 Mg2+ the enzyme essentially requires Mg2+ or Mn2+ Mycobacterium tuberculosis
1.1.1.267 Mn2+ the enzyme essentially requires Mg2+ or Mn2+ Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.267 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ Mycobacterium tuberculosis
-
2-C-methyl-D-erythritol 4-phosphate + NADP+
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.267 Mycobacterium tuberculosis
-
gene Rv2870c
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.267 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography to over 90% purity Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.267 1-deoxy-D-xylulose 5-phosphate + NADPH + H+
-
Mycobacterium tuberculosis 2-C-methyl-D-erythritol 4-phosphate + NADP+
-
?
1.1.1.267 additional information development and evaluation of a high-throughput screening assay for simultaneous selection of inhibitors of the enzyme, overview Mycobacterium tuberculosis ?
-
?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.267 1-deoxy-D-xylulose 5-phosphate reductoisomerase
-
Mycobacterium tuberculosis
1.1.1.267 DXR
-
Mycobacterium tuberculosis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.267 25
-
assay at Mycobacterium tuberculosis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.267 7.5
-
assay at Mycobacterium tuberculosis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.267 NADPH
-
Mycobacterium tuberculosis

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.1.1.267 0.000054
-
pH 7.5, 25°C, recombinant enzyme Mycobacterium tuberculosis fosmidomycin