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Literature summary extracted from

  • Mills, S.A.; Brown, D.E.; Dang, K.; Sommer, D.; Bitsimis, A.; Nguyen, J.; Dooley, D.M.
    Cobalt substitution supports an inner-sphere electron transfer mechanism for oxygen reduction in pea seedling amine oxidase (2012), J. Biol. Inorg. Chem., 17, 507-515.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.3.21 0.021
-
 O2 copper-reconstituted wild type enzyme, with putrescine as cosubstrate, in 100 mM potassium phosphate buffer, pH 7.2, 37°C Pisum sativum
1.4.3.21 0.026
-
 O2 wild type enzyme, with putrescine as cosubstrate, in 100 mM potassium phosphate buffer, pH 7.2, 37°C Pisum sativum
1.4.3.21 0.29
-
 putrescine copper-reconstituted wild type enzyme, in 100 mM potassium phosphate buffer, pH 7.2, 37°C Pisum sativum
1.4.3.21 0.57
-
 putrescine wild type enzyme, in 100 mM potassium phosphate buffer, pH 7.2, 37°C Pisum sativum
1.4.3.21 1
-
 O2 cobalt-substituted wild type enzyme, with putrescine as cosubstrate, in 100 mM potassium phosphate buffer, pH 7.2, 37°C Pisum sativum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.4.3.21 Co2+ cobalt-substituted enzyme displays nominal catalytic activity Pisum sativum
1.4.3.21 Cu2+ the wild type enzyme contains 1.4 mol copper per monomer Pisum sativum

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.21 Pisum sativum
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.4.3.21 seedling
-
 Pisum sativum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.3.21 0.016
-
 copper-depleted wild type enzyme, with 4-N,N-dimethylaminomethyl benzylamine as substrate, in 100 mM potassium phosphate buffer, pH 7.2, 37°C Pisum sativum
1.4.3.21 0.38
-
 cobalt-substituted wild type enzyme, with 4-N,N-dimethylaminomethyl benzylamine as substrate, in 100 mM potassium phosphate buffer, pH 7.2, 37°C Pisum sativum
1.4.3.21 7
-
 copper-reconstituted wild type enzyme, with 4-N,N-dimethylaminomethyl benzylamine as substrate, in 100 mM potassium phosphate buffer, pH 7.2, 37°C Pisum sativum
1.4.3.21 13.6
-
 wild type enzyme, with 4-N,N-dimethylaminomethyl benzylamine as substrate, in 100 mM potassium phosphate buffer, pH 7.2, 37°C Pisum sativum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.21 4-N,N-dimethylaminomethyl benzylamine + H2O + O2
-
 Pisum sativum ?
-
 ?
1.4.3.21 putrescine + H2O + O2
-
 Pisum sativum ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.4.3.21 CAO
-
 Pisum sativum
1.4.3.21 Copper amine oxidase
-
 Pisum sativum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.4.3.21 7.1
-
 O2 cobalt-substituted wild type enzyme, with putrescine as cosubstrate, in 100 mM potassium phosphate buffer, pH 7.2, 37°C Pisum sativum
1.4.3.21 82
-
 putrescine copper-reconstituted wild type enzyme, in 100 mM potassium phosphate buffer, pH 7.2, 37°C Pisum sativum
1.4.3.21 99
-
 O2 copper-reconstituted wild type enzyme, with putrescine as cosubstrate, in 100 mM potassium phosphate buffer, pH 7.2, 37°C Pisum sativum
1.4.3.21 152
-
 O2 wild type enzyme, with putrescine as cosubstrate, in 100 mM potassium phosphate buffer, pH 7.2, 37°C Pisum sativum
1.4.3.21 160
-
 putrescine wild type enzyme, in 100 mM potassium phosphate buffer, pH 7.2, 37°C Pisum sativum

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.3.21 2,4,5-trihydroxyphenylalanine quinone
-
 Pisum sativum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.4.3.21 7.1
-
 O2 cobalt-substituted wild type enzyme, with putrescine as cosubstrate, in 100 mM potassium phosphate buffer, pH 7.2, 37°C Pisum sativum
1.4.3.21 270
-
 putrescine wild type enzyme, in 100 mM potassium phosphate buffer, pH 7.2, 37°C Pisum sativum
1.4.3.21 280
-
 putrescine copper-reconstituted wild type enzyme, in 100 mM potassium phosphate buffer, pH 7.2, 37°C Pisum sativum
1.4.3.21 3320
-
 O2 wild type enzyme, with putrescine as cosubstrate, in 100 mM potassium phosphate buffer, pH 7.2, 37°C Pisum sativum
1.4.3.21 4640
-
 O2 copper-reconstituted wild type enzyme, with putrescine as cosubstrate, in 100 mM potassium phosphate buffer, pH 7.2, 37°C Pisum sativum