EC Number | Cloned (Comment) | Organism |
---|---|---|
6.2.1.40 | expression in Escherichia coli | Metallosphaera sedula |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
6.2.1.40 | W424G | wild-type enzyme shows no activity with 4-hydroxybutanoate, mutant enzyme W424G shows activity | Metallosphaera sedula |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.2.1.40 | 1.13 | - |
4-hydroxybutanoate | pH 7.9, 75°C, mutant enzyme W424G | Metallosphaera sedula | |
6.2.1.40 | 1.54 | - |
4-hydroxybutanoate | pH 7.9, 75°C | Metallosphaera sedula | |
6.2.1.40 | 2 | - |
4-hydroxybutanoate | pH 7.9, 75°C | Metallosphaera sedula |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
6.2.1.40 | 62000 | - |
x * 62000, calculated from sequence | Metallosphaera sedula |
6.2.1.40 | 64000 | - |
x * 64000, SDS-PAGE | Metallosphaera sedula |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.2.1.40 | ATP + 4-hydroxybutanoate + CoA | Metallosphaera sedula | the enzyme is involved in the 3-hydroxypropionate/4-hydroxybutyrate cycle | AMP + diphosphate + 4-hydroxybutanoyl-CoA | - |
? | |
6.2.1.40 | ATP + 4-hydroxybutanoate + CoA | Metallosphaera sedula DSM 5348 | the enzyme is involved in the 3-hydroxypropionate/4-hydroxybutyrate cycle | AMP + diphosphate + 4-hydroxybutanoyl-CoA | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.2.1.40 | Metallosphaera sedula | A4YDR9 | - |
- |
6.2.1.40 | Metallosphaera sedula | A4YDT1 | - |
- |
6.2.1.40 | Metallosphaera sedula | A4YGF9 | accession number for wild-type enzyme, which shows no activity with 4-hydroxybutanoate, mutant enzyme W424G shows activity | - |
6.2.1.40 | Metallosphaera sedula DSM 5348 | A4YDR9 | - |
- |
6.2.1.40 | Metallosphaera sedula DSM 5348 | A4YDT1 | - |
- |
6.2.1.40 | Metallosphaera sedula DSM 5348 | A4YGF9 | accession number for wild-type enzyme, which shows no activity with 4-hydroxybutanoate, mutant enzyme W424G shows activity | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
6.2.1.40 | - |
Metallosphaera sedula |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
6.2.1.40 | culture condition | cells grown under strict H2-CO2 autotrophy | Metallosphaera sedula | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
6.2.1.40 | 0.22 | - |
substrate: 4-hydroxybutanoate, pH 7.9, 75°C | Metallosphaera sedula |
6.2.1.40 | 1.7 | - |
substrate: 4-hydroxybutanoate, pH 7.9, 75°C | Metallosphaera sedula |
6.2.1.40 | 2.3 | - |
substrate: 4-hydroxybutanoate, pH 7.9, 75°C, mutant enzyme W424G | Metallosphaera sedula |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.2.1.40 | ATP + 4-hydroxybutanoate + CoA | the enzyme is involved in the 3-hydroxypropionate/4-hydroxybutyrate cycle | Metallosphaera sedula | AMP + diphosphate + 4-hydroxybutanoyl-CoA | - |
? | |
6.2.1.40 | ATP + 4-hydroxybutanoate + CoA | the enzyme has a clear preference for unsubstituted straight chain organic acids with a chain length of four or five carbons. No activity is detected with the six-carbon hexanoic acid | Metallosphaera sedula | AMP + diphosphate + 4-hydroxybutanoyl-CoA | - |
? | |
6.2.1.40 | ATP + 4-hydroxybutanoate + CoA | tzhe enzyme is also a promiscuous acetate/propionate ligase | Metallosphaera sedula | AMP + diphosphate + 4-hydroxybutanoyl-CoA | - |
? | |
6.2.1.40 | ATP + 4-hydroxybutanoate + CoA | wild-type enzyme shows no activity, mutant enzyme W424G shows activity | Metallosphaera sedula | AMP + diphosphate + 4-hydroxybutanoyl-CoA | - |
? | |
6.2.1.40 | ATP + 4-hydroxybutanoate + CoA | the enzyme is involved in the 3-hydroxypropionate/4-hydroxybutyrate cycle | Metallosphaera sedula DSM 5348 | AMP + diphosphate + 4-hydroxybutanoyl-CoA | - |
? | |
6.2.1.40 | ATP + 4-hydroxybutanoate + CoA | the enzyme has a clear preference for unsubstituted straight chain organic acids with a chain length of four or five carbons. No activity is detected with the six-carbon hexanoic acid | Metallosphaera sedula DSM 5348 | AMP + diphosphate + 4-hydroxybutanoyl-CoA | - |
? | |
6.2.1.40 | ATP + 4-hydroxybutanoate + CoA | tzhe enzyme is also a promiscuous acetate/propionate ligase | Metallosphaera sedula DSM 5348 | AMP + diphosphate + 4-hydroxybutanoyl-CoA | - |
? | |
6.2.1.40 | ATP + 4-hydroxybutanoate + CoA | wild-type enzyme shows no activity, mutant enzyme W424G shows activity | Metallosphaera sedula DSM 5348 | AMP + diphosphate + 4-hydroxybutanoyl-CoA | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
6.2.1.40 | ? | x * 64000, SDS-PAGE | Metallosphaera sedula |
6.2.1.40 | ? | x * 62000, calculated from sequence | Metallosphaera sedula |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
6.2.1.40 | 4-hydroxybutyrate-CoA synthetase | - |
Metallosphaera sedula |
6.2.1.40 | Msed_0394 | locus name | Metallosphaera sedula |
6.2.1.40 | Msed_0406 | locus name | Metallosphaera sedula |
6.2.1.40 | Msed_1353 | locus name | Metallosphaera sedula |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
6.2.1.40 | 75 | - |
assay at | Metallosphaera sedula |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.2.1.40 | 0.24 | - |
4-hydroxybutanoate | pH 7.9, 75°C | Metallosphaera sedula | |
6.2.1.40 | 1.8 | - |
4-hydroxybutanoate | pH 7.9, 75°C | Metallosphaera sedula | |
6.2.1.40 | 2.5 | - |
4-hydroxybutanoate | pH 7.9, 75°C, mutant enzyme W424G | Metallosphaera sedula |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
6.2.1.40 | 7.9 | - |
assay at | Metallosphaera sedula |
EC Number | General Information | Comment | Organism |
---|---|---|---|
6.2.1.40 | physiological function | the enzyme is involved in the 3-hydroxypropionate/4-hydroxybutyrate cycle | Metallosphaera sedula |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.2.1.40 | 0.16 | - |
4-hydroxybutanoate | pH 7.9, 75°C | Metallosphaera sedula | |
6.2.1.40 | 0.91 | - |
4-hydroxybutanoate | pH 7.9, 75°C | Metallosphaera sedula | |
6.2.1.40 | 2.18 | - |
4-hydroxybutanoate | pH 7.9, 75°C, mutant enzyme W424G | Metallosphaera sedula |