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Literature summary extracted from

  • Nihira, T.; Suzuki, E.; Kitaoka, M.; Nishimoto, M.; Ohtsubo, K.; Nakai, H.
    Discovery of beta-1,4-D-mannosyl-N-acetyl-D-glucosamine phosphorylase involved in the metabolism of N-glycans (2013), J. Biol. Chem., 288, 27366-27374.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.320 expressed in Escherichia coli DH5alpha Bacteroides thetaiotaomicron
2.4.1.320 expression in Escherichia coli Bacteroides thetaiotaomicron

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.320 0.6
-
 phosphate pH 5.5, 30°C, cosubstrate: 4-O-beta-D-mannopyranosyl-N-acetyl-D-glucosamine, phosphorolytic reaction Bacteroides thetaiotaomicron
2.4.1.320 2.1
-
 4-O-beta-D-mannopyranosyl-N-acetyl-D-glucosamine pH 5.5, 30°C, cosubstrate: phosphate, phosphorolytic reaction Bacteroides thetaiotaomicron
2.4.1.320 2.6
-
 alpha-D-mannose 1-phosphate pH 5.5, 30°C Bacteroides thetaiotaomicron
2.4.1.320 2.6
-
 alpha-D-mannose 1-phosphate pH 5.5, 30°C, cosubstrate: N-acetyl-D-glucosamine, synthetic reaction Bacteroides thetaiotaomicron
2.4.1.320 2.7
-
 4-O-beta-N-acetyl-D-glucosaminyl-N-acetyl-D-glucosamine pH 5.5, 30°C Bacteroides thetaiotaomicron
2.4.1.320 3.8
-
 N-acetyl-D-glucosamine pH 5.5, 30°C Bacteroides thetaiotaomicron
2.4.1.320 3.8
-
 N-acetyl-D-glucosamine pH 5.5, 30°C, synthetic reaction Bacteroides thetaiotaomicron
2.4.1.320 27
-
 N,N'-diacetylchitobiose pH 5.5, 30°C, synthetic reaction Bacteroides thetaiotaomicron

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.320 cytoplasm
-
 Bacteroides thetaiotaomicron 5737
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.320 35000
-
 4 * 35000, SDS-PAGE Bacteroides thetaiotaomicron
2.4.1.320 35000
-
 4 * 35000, SDS-PAGE, 4 * 37823, amino acid sequence Bacteroides thetaiotaomicron
2.4.1.320 37823
-
 4 * 35000, SDS-PAGE, 4 * 37823, amino acid sequence Bacteroides thetaiotaomicron
2.4.1.320 37823
-
 4 * 37823, calculated from sequence Bacteroides thetaiotaomicron
2.4.1.320 147000
-
 gel filtration Bacteroides thetaiotaomicron

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.320 4-O-beta-D-mannopyranosyl-N-acetyl-D-glucosamine + phosphate Bacteroides thetaiotaomicron the enzyme is involved in the degradation of host-derived N-glycans N-acetyl-D-glucosamine + alpha-D-mannose 1-phosphate
-
 r
2.4.1.320 4-O-beta-D-mannopyranosyl-N-acetyl-D-glucosamine + phosphate Bacteroides thetaiotaomicron VPI-5482 the enzyme is involved in the degradation of host-derived N-glycans N-acetyl-D-glucosamine + alpha-D-mannose 1-phosphate
-
 r
2.4.1.320 4-O-beta-D-mannosyl-N-acetyl-D-glucosamine + phosphate Bacteroides thetaiotaomicron
-
 N-acetyl-D-glucosamine + alpha-D-mannose 1-phosphate
-
 r
2.4.1.320 4-O-beta-D-mannosyl-N-acetyl-D-glucosamine + phosphate Bacteroides thetaiotaomicron VPI-5482
-
 N-acetyl-D-glucosamine + alpha-D-mannose 1-phosphate
-
 r
2.4.1.320 N-acetyl-D-glucosamine + alpha-D-mannose 1-phosphate Bacteroides thetaiotaomicron
-
 4-O-beta-D-mannosyl-N-acetyl-D-glucosamine + phosphate
-
 r
2.4.1.320 N-acetyl-D-glucosamine + alpha-D-mannose 1-phosphate Bacteroides thetaiotaomicron VPI-5482
-
 4-O-beta-D-mannosyl-N-acetyl-D-glucosamine + phosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.320 Bacteroides thetaiotaomicron Q8A8X4
-
-
2.4.1.320 Bacteroides thetaiotaomicron Q8A8Y4
-
-
2.4.1.320 Bacteroides thetaiotaomicron VPI-5482 Q8A8X4
-
-
2.4.1.320 Bacteroides thetaiotaomicron VPI-5482 Q8A8Y4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.320
-
 Bacteroides thetaiotaomicron

Reaction

EC Number Reaction Comment Organism Reaction ID
2.4.1.320 4-O-beta-D-mannopyranosyl-N-acetyl-D-glucosamine + phosphate = N-acetyl-D-glucosamine + alpha-D-mannose 1-phosphate sequential Bi Bi mechanism Bacteroides thetaiotaomicron
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.320 30
-
 pH 5.5, 30°C, substrate: N,N'-diacetylchitobiose Bacteroides thetaiotaomicron
2.4.1.320 258
-
 pH 5.5, 30°C, substrate: N-acetyl-D-glucosamine Bacteroides thetaiotaomicron

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.320 4-O-beta-D-mannopyranosyl-N-acetyl-D-glucosamine + phosphate the enzyme is involved in the degradation of host-derived N-glycans Bacteroides thetaiotaomicron N-acetyl-D-glucosamine + alpha-D-mannose 1-phosphate
-
 r
2.4.1.320 4-O-beta-D-mannopyranosyl-N-acetyl-D-glucosamine + phosphate the enzyme is involved in the degradation of host-derived N-glycans Bacteroides thetaiotaomicron VPI-5482 N-acetyl-D-glucosamine + alpha-D-mannose 1-phosphate
-
 r
2.4.1.320 4-O-beta-D-mannosyl-N-acetyl-D-glucosamine + phosphate
-
 Bacteroides thetaiotaomicron N-acetyl-D-glucosamine + alpha-D-mannose 1-phosphate
-
 r
2.4.1.320 4-O-beta-D-mannosyl-N-acetyl-D-glucosamine + phosphate
-
 Bacteroides thetaiotaomicron VPI-5482 N-acetyl-D-glucosamine + alpha-D-mannose 1-phosphate
-
 r
2.4.1.320 4-O-beta-N-acetyl-D-glucosaminyl-N-acetyl-D-glucosamine + alpha-D-mannose 1-phosphate
-
 Bacteroides thetaiotaomicron beta-N-acetyl-D-glucosaminyl-(1->4)-beta-N-acetyl-D-glucosaminyl-(1->4)-D-mannose + phosphate
-
 r
2.4.1.320 4-O-beta-N-acetyl-D-glucosaminyl-N-acetyl-D-glucosamine + alpha-D-mannose 1-phosphate
-
 Bacteroides thetaiotaomicron VPI-5482 beta-N-acetyl-D-glucosaminyl-(1->4)-beta-N-acetyl-D-glucosaminyl-(1->4)-D-mannose + phosphate
-
 r
2.4.1.320 beta-N-acetyl-D-glucosaminyl-(1->4)-beta-N-acetyl-D-glucosaminyl-(1->4)-D-mannose + phosphate
-
 Bacteroides thetaiotaomicron 4-O-beta-N-acetyl-D-glucosaminyl-N-acetyl-D-glucosamine + alpha-D-mannose 1-phosphate
-
 r
2.4.1.320 beta-N-acetyl-D-glucosaminyl-(1->4)-beta-N-acetyl-D-glucosaminyl-(1->4)-D-mannose + phosphate
-
 Bacteroides thetaiotaomicron VPI-5482 4-O-beta-N-acetyl-D-glucosaminyl-N-acetyl-D-glucosamine + alpha-D-mannose 1-phosphate
-
 r
2.4.1.320 additional information very low activity with D-glucose or D-mannose as acceptor and alpha-D-mannose 1-phosphate as donor Bacteroides thetaiotaomicron ?
-
 ?
2.4.1.320 additional information very low activity with D-glucose or D-mannose as acceptor and alpha-D-mannose 1-phosphate as donor Bacteroides thetaiotaomicron VPI-5482 ?
-
 ?
2.4.1.320 N,N'-diacetylchitobiose + alpha-D-mannose 1-phosphate
-
 Bacteroides thetaiotaomicron beta-1,4-D-mannopyranosyl-beta-1,4-N-acetyl-D-glucosaminyl-beta-1,4-N-acetyl-D-glucosamine + phosphate
-
 r
2.4.1.320 N,N'-diacetylchitobiose + alpha-D-mannose 1-phosphate
-
 Bacteroides thetaiotaomicron VPI-5482 beta-1,4-D-mannopyranosyl-beta-1,4-N-acetyl-D-glucosaminyl-beta-1,4-N-acetyl-D-glucosamine + phosphate
-
 r
2.4.1.320 N-acetyl-D-glucosamine + alpha-D-mannose 1-phosphate
-
 Bacteroides thetaiotaomicron 4-O-beta-D-mannosyl-N-acetyl-D-glucosamine + phosphate
-
 r
2.4.1.320 N-acetyl-D-glucosamine + alpha-D-mannose 1-phosphate
-
 Bacteroides thetaiotaomicron VPI-5482 4-O-beta-D-mannosyl-N-acetyl-D-glucosamine + phosphate
-
 r
2.4.1.320 N-acetyl-D-glucosamine + alpha-D-mannose 1-phosphate
-
 Bacteroides thetaiotaomicron 4-O-beta-D-mannopyranosyl-N-acetyl-D-glucosamine + phosphate
-
 r
2.4.1.320 N-acetyl-D-glucosamine + alpha-D-mannose 1-phosphate
-
 Bacteroides thetaiotaomicron VPI-5482 4-O-beta-D-mannopyranosyl-N-acetyl-D-glucosamine + phosphate
-
 r

Subunits

EC Number Subunits Comment Organism
2.4.1.320 homotetramer 4 * 35000, SDS-PAGE Bacteroides thetaiotaomicron
2.4.1.320 homotetramer 4 * 35000, SDS-PAGE, 4 * 37823, amino acid sequence Bacteroides thetaiotaomicron
2.4.1.320 homotetramer 4 * 37823, calculated from sequence Bacteroides thetaiotaomicron

Synonyms

EC Number Synonyms Comment Organism
2.4.1.320 beta-1,4-D-mannosyl-N-acetyl-D-glucosamine phosphorylase
-
 Bacteroides thetaiotaomicron
2.4.1.320 BT1033
-
 Bacteroides thetaiotaomicron

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.320 30
-
 assay at Bacteroides thetaiotaomicron

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.320 55
-
 15 min, stable up to Bacteroides thetaiotaomicron
2.4.1.320 55
-
 15 min stable up to Bacteroides thetaiotaomicron

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.320 22
-
 4-O-beta-N-acetyl-D-glucosaminyl-N-acetyl-D-glucosamine pH 5.5, 30°C Bacteroides thetaiotaomicron
2.4.1.320 22
-
 N,N'-diacetylchitobiose pH 5.5, 30°C, synthetic reaction Bacteroides thetaiotaomicron
2.4.1.320 25
-
 alpha-D-mannose 1-phosphate pH 5.5, 30°C Bacteroides thetaiotaomicron
2.4.1.320 25
-
 alpha-D-mannose 1-phosphate pH 5.5, 30°C, cosubstrate: N-acetyl-D-glucosamine, synthetic reaction Bacteroides thetaiotaomicron
2.4.1.320 37
-
 N-acetyl-D-glucosamine pH 5.5, 30°C Bacteroides thetaiotaomicron
2.4.1.320 37
-
 N-acetyl-D-glucosamine pH 5.5, 30°C, synthetic reaction Bacteroides thetaiotaomicron
2.4.1.320 240
-
 phosphate pH 5.5, 30°C, cosubstrate: 4-O-beta-D-mannopyranosyl-N-acetyl-D-glucosamine, phosphorolytic reaction Bacteroides thetaiotaomicron
2.4.1.320 240
-
 4-O-beta-D-mannopyranosyl-N-acetyl-D-glucosamine pH 5.5, 30°C, cosubstrate: phosphate, phosphorolytic reaction Bacteroides thetaiotaomicron

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.320 5.5
-
-
 Bacteroides thetaiotaomicron
2.4.1.320 5.5
-
 phosphorolytic reaction Bacteroides thetaiotaomicron
2.4.1.320 5.5
-
 synthetic reaction Bacteroides thetaiotaomicron

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.4.1.320 4.5 10.5 4°C, 24 h, stable Bacteroides thetaiotaomicron
2.4.1.320 4.5 10.5 4°C, 24 h stable Bacteroides thetaiotaomicron

General Information

EC Number General Information Comment Organism
2.4.1.320 physiological function the enzyme is involved in the degradation of host-derived N-glycans Bacteroides thetaiotaomicron

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.1.320 0.8
-
 N,N'-diacetylchitobiose pH 5.5, 30°C, synthetic reaction Bacteroides thetaiotaomicron
2.4.1.320 1.9
-
 beta-1,4-D-mannopyranosyl-beta-1,4-N-acetyl-D-glucosaminyl-beta-1,4-N-acetyl-D-glucosamine pH 5.5, 30°C, cosubstrate: phosphate, phosphorolytic reaction Bacteroides thetaiotaomicron
2.4.1.320 9.6
-
 alpha-D-mannose 1-phosphate pH 5.5, 30°C, cosubstrate: N-acetyl-D-glucosamine, synthetic reaction Bacteroides thetaiotaomicron
2.4.1.320 9.6
-
 N-acetyl-D-glucosamine pH 5.5, 30°C, synthetic reaction Bacteroides thetaiotaomicron
2.4.1.320 110
-
 4-O-beta-D-mannopyranosyl-N-acetyl-D-glucosamine pH 5.5, 30°C, cosubstrate: phosphate, phosphorolytic reaction Bacteroides thetaiotaomicron