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Literature summary extracted from

  • Yang, S.; Jan, Y.H.; Gray, J.P.; Mishin, V.; Heck, D.E.; Laskin, D.L.; Laskin, J.D.
    Sepiapterin Reductase Mediates Chemical Redox Cycling in Lung Epithelial Cells (2013), J. Biol. Chem., 288, 19221-19237.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.153 expressed in Escherichia coli Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.153 D257H mutant shows completely inhibited sepiapterin reduction. Mutation has only minimal effects on redox cycling Homo sapiens
1.1.1.153 DELTA257-261 deletion of the C-terminal 5 amino acids almost completely eliminates enzyme activity. For redox cycling, the catalytic efficacy decreases to less than 1% of the wild type enzyme Homo sapiens
1.1.1.153 G14S mutations in Gly14 and Gly18 in the NADPH binding motif of sepiapterin reductase results in almost complete loss of the ability to reduce sepiapterin, and a 65-75% decrease in redox cycling. For both of these mutations, the catalytic efficiencies for redox cycling decreases to 0.2% of wild type sepiapterin reductase Homo sapiens
1.1.1.153 G18D mutations in Gly14 and Gly18 in the NADPH binding motif of sepiapterin reductase results in almost complete loss of the ability to reduce sepiapterin, and a 65-75% decrease in redox cycling. For both of these mutations, the catalytic efficiencies for redox cycling decreases to 0.2% of wild type sepiapterin reductase Homo sapiens
1.1.1.153 K174L catalytic efficiencies (Kcat/Km) for sepiapterin reduction of S157A mutant and K174L mutant decreases to 1.8% and 0.8% of wild type sepiapterin reductase, respectively, and for redox cycling to 6.8% and 1.4%, respectively Homo sapiens
1.1.1.153 M205G mutation leads to marked reductions in the activities of both sepiapterin reduction and redox cycling. The catalytic efficiency of N99A and M205G for sepiapterin reduction decreases to approximately 1% and 5%, respectively, and for redox cycling, 5% and 25%, respectively, when compared to the wild type enzyme Homo sapiens
1.1.1.153 N99A mutation leads to marked reductions in the activities of both sepiapterin reduction and redox cycling. The catalytic efficiency of N99A and M205G for sepiapterin reduction decreases to approximately 1% and 5%, respectively, and for redox cycling, 5% and 25%, respectively, when compared to the wild type enzyme Homo sapiens
1.1.1.153 R42G mutation leads to a 90% reduction in sepiapterin reduction activity and a 50% reduction in redox cycling activity. The catalytic efficiencies for this mutant decreases to 2% and 7% of wild type sepiapterin reductase for sepiapterin reduction and redox cycling, respectively Homo sapiens
1.1.1.153 S157A catalytic efficiencies (Kcat/Km) for sepiapterin reduction of S157A mutant and K174L mutant decreases to 1.8% and 0.8% of wild type sepiapterin reductase, respectively, and for redox cycling to 6.8% and 1.4%, respectively Homo sapiens
1.1.1.153 Y259A mutation of Tyr259, a unique potential phosphorylation site in the C-terminal substrate transfer motif, has no major effects on sepiapterin reduction and redox cycling activity Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.153 1,2-naphthoquinone
-
 Homo sapiens
1.1.1.153 9,10-phenanthrenequinone
-
 Homo sapiens
1.1.1.153 benzoquinone
-
 Homo sapiens
1.1.1.153 dicoumarol
-
 Homo sapiens
1.1.1.153 indomethacin
-
 Homo sapiens
1.1.1.153 N-acetylserotonin
-
 Homo sapiens
1.1.1.153 phenylquinone
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.153 0.0019
-
 9,10-phenanthrenequinone mutant D257H, pH 6.4, 37°C Homo sapiens
1.1.1.153 0.0021
-
 1,2-naphthoquinone wild-type, pH 6.4, 37°C Homo sapiens
1.1.1.153 0.0032
-
 9,10-phenanthrenequinone mutant S157A, pH 6.4, 37°C Homo sapiens
1.1.1.153 0.0088
-
 9,10-phenanthrenequinone wild-type, pH 6.4, 37°C Homo sapiens
1.1.1.153 0.0133
-
 9,10-phenanthrenequinone mutant M205G, pH 6.4, 37°C Homo sapiens
1.1.1.153 0.0196
-
 9,10-phenanthrenequinone mutant Y259A, pH 6.4, 37°C Homo sapiens
1.1.1.153 0.025
-
 sepiapterin mutant Y259A, pH 6.4, 37°C Homo sapiens
1.1.1.153 0.025
-
 sepiapterin wild-type, pH 6.4, 37°C Homo sapiens
1.1.1.153 0.027
-
 9,10-phenanthrenequinone mutant K174L, pH 6.4, 37°C Homo sapiens
1.1.1.153 0.027
-
 1,4-Naphthoquinone wild-type, pH 6.4, 37°C Homo sapiens
1.1.1.153 0.045
-
 sepiapterin mutant R42G, pH 6.4, 37°C Homo sapiens
1.1.1.153 0.0501
-
 9,10-phenanthrenequinone mutant G14S, pH 6.4, 37°C Homo sapiens
1.1.1.153 0.0501
-
 9,10-phenanthrenequinone mutant R42G, pH 6.4, 37°C Homo sapiens
1.1.1.153 0.0503
-
 sepiapterin mutant S157A, pH 6.4, 37°C Homo sapiens
1.1.1.153 0.0606
-
 sepiapterin mutant D257H, pH 6.4, 37°C Homo sapiens
1.1.1.153 0.086
-
 2,3-dimethoxynaphthalene-1,4-dione wild-type, pH 6.4, 37°C Homo sapiens
1.1.1.153 0.0872
-
 menadione wild-type, pH 6.4, 37°C Homo sapiens
1.1.1.153 0.094
-
 sepiapterin mutant N99A, pH 6.4, 37°C Homo sapiens
1.1.1.153 0.116
-
 9,10-phenanthrenequinone mutant DELTA257-261, pH 6.4, 37°C Homo sapiens
1.1.1.153 0.126
-
 9,10-phenanthrenequinone mutant N99A, pH 6.4, 37°C Homo sapiens
1.1.1.153 0.133
-
 sepiapterin mutant M205G, pH 6.4, 37°C Homo sapiens
1.1.1.153 0.169
-
 sepiapterin mutant K174L, pH 6.4, 37°C Homo sapiens
1.1.1.153 0.464
-
 9,10-phenanthrenequinone mutant G18D, pH 6.4, 37°C Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.153 28000
-
 SDS-PAGE Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.153 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.153 lung epithelial cell
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.153 1.8
-
 pH 6.7, 37°C Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.153 1,2-naphthoquinone + NADPH
-
 Homo sapiens ?
-
 ?
1.1.1.153 1,4-naphthoquinone + NADPH + H+
-
 Homo sapiens ?
-
 ?
1.1.1.153 2,3-dimethoxynaphthalene-1,4-dione + NADPH
-
 Homo sapiens ?
-
 ?
1.1.1.153 9,10-phenanthrenequinone + NADPH
-
 Homo sapiens ?
-
 ?
1.1.1.153 menadione + NADPH
-
 Homo sapiens ?
-
 ?
1.1.1.153 sepiapterin + NADPH + H+
-
 Homo sapiens 7,8-dihydrobiopterin + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.153 sepiapterin reductase
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.153 37
-
 assay at Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.153 0.0048
-
 9,10-phenanthrenequinone mutant S157A, pH 6.4, 37°C Homo sapiens
1.1.1.153 0.0053
-
 sepiapterin mutant N99A, pH 6.4, 37°C Homo sapiens
1.1.1.153 0.0056
-
 9,10-phenanthrenequinone mutant G14S, pH 6.4, 37°C Homo sapiens
1.1.1.153 0.0058
-
 9,10-phenanthrenequinone mutant DELTA257-261, pH 6.4, 37°C Homo sapiens
1.1.1.153 0.0058
-
 sepiapterin mutant S157A, pH 6.4, 37°C Homo sapiens
1.1.1.153 0.027
-
 9,10-phenanthrenequinone mutant K174L, pH 6.4, 37°C Homo sapiens
1.1.1.153 0.048
-
 9,10-phenanthrenequinone mutant G18D, pH 6.4, 37°C Homo sapiens
1.1.1.153 0.053
-
 sepiapterin mutant R42G, pH 6.4, 37°C Homo sapiens
1.1.1.153 0.09
-
 sepiapterin mutant K174L, pH 6.4, 37°C Homo sapiens
1.1.1.153 0.098
-
 9,10-phenanthrenequinone mutant R42G, pH 6.4, 37°C Homo sapiens
1.1.1.153 0.107
-
 9,10-phenanthrenequinone mutant D257H, pH 6.4, 37°C Homo sapiens
1.1.1.153 0.146
-
 9,10-phenanthrenequinone wild-type, pH 6.4, 37°C Homo sapiens
1.1.1.153 0.156
-
 sepiapterin mutant D257H, pH 6.4, 37°C Homo sapiens
1.1.1.153 0.22
-
 9,10-phenanthrenequinone mutant M205G, pH 6.4, 37°C Homo sapiens
1.1.1.153 0.29
-
 9,10-phenanthrenequinone mutant N99A, pH 6.4, 37°C Homo sapiens
1.1.1.153 0.428
-
 sepiapterin mutant M205G, pH 6.4, 37°C Homo sapiens
1.1.1.153 0.45
-
 1,4-Naphthoquinone wild-type, pH 6.4, 37°C Homo sapiens
1.1.1.153 0.6
-
 sepiapterin mutant Y259A, pH 6.4, 37°C Homo sapiens
1.1.1.153 0.627
-
 9,10-phenanthrenequinone wild-type, pH 6.4, 37°C Homo sapiens
1.1.1.153 0.627
-
 1,2-naphthoquinone wild-type, pH 6.4, 37°C Homo sapiens
1.1.1.153 0.805
-
 9,10-phenanthrenequinone mutant Y259A, pH 6.4, 37°C Homo sapiens
1.1.1.153 1.43
-
 2,3-dimethoxynaphthalene-1,4-dione wild-type, pH 6.4, 37°C Homo sapiens
1.1.1.153 1.45
-
 menadione wild-type, pH 6.4, 37°C Homo sapiens
1.1.1.153 1.61
-
 sepiapterin wild-type, pH 6.4, 37°C Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.153 6.4
-
 assay at Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.1.153 0.0005
-
 phenylquinone wild-type, pH 6.7, 37°C Homo sapiens
1.1.1.153 0.0009
-
 N-acetylserotonin wild-type, pH 6.7, 37°C Homo sapiens
1.1.1.153 0.0009
-
 benzoquinone wild-type, pH 6.7, 37°C Homo sapiens
1.1.1.153 0.0027
-
 indomethacin wild-type, pH 6.7, 37°C Homo sapiens

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.1.1.153 0.0002
-
 wild-type, pH 6.7, 37°C Homo sapiens dicoumarol
1.1.1.153 0.0016
-
 wild-type, pH 6.7, 37°C Homo sapiens phenylquinone
1.1.1.153 0.0026
-
 wild-type, pH 6.7, 37°C Homo sapiens N-acetylserotonin
1.1.1.153 0.0028
-
 wild-type, pH 6.7, 37°C Homo sapiens benzoquinone
1.1.1.153 0.0036
-
 wild-type, pH 6.7, 37°C Homo sapiens 9,10-phenanthrenequinone
1.1.1.153 0.0081
-
 wild-type, pH 6.7, 37°C Homo sapiens indomethacin
1.1.1.153 0.0089
-
 wild-type, pH 6.7, 37°C Homo sapiens 1,2-naphthoquinone