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Literature summary extracted from

  • Ringel, P.; Krausze, J.; Van Heuvel, J.; Curth, U.; Pierik, A.; Herzog, S.; Mendel, R.; Kruse, T.
    Biochemical characterization of molybdenum cofactor-free nitrate reductase from Neurospora crassa (2013), J. Biol. Chem., 288, 14657-14671.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7.1.3 0.29
-
 nitrate pH 7.2, temperature not specified in the publication Neurospora crassa

Organism

EC Number Organism UniProt Comment Textmining
1.7.1.3 Neurospora crassa P08619
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7.1.3 1.24
-
 pH 7.2, temperature not specified in the publication Neurospora crassa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.1.3 nitrate + NADPH + H+
-
 Neurospora crassa nitrite + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.7.1.3 dimer in presence of molybdenum cofactor, enzyme forms a dimer, gel filtration, sedimentation velocity analysis Neurospora crassa

Synonyms

EC Number Synonyms Comment Organism
1.7.1.3 nit-3
-
 Neurospora crassa

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.1.3 FAD involved in electron transfer from NADPH to the enzyme molybdenum center where reduction of nitrate to nitrite takes place Neurospora crassa
1.7.1.3 heme involved in electron transfer from NADPH to the enzyme molybdenum center where reduction of nitrate to nitrite takes place Neurospora crassa
1.7.1.3 molybdenum cofactor cofactor is necessary and sufficient to induce dimer formation. The molybdenum center of nitrate reductase reconstituted in vitro from apo-enzyme and cofactor shows an EPR spectrum identical to holo-enzyme. Insertion of this cofactor into the enzyme occurs independent from the insertion of any other NR redox cofactor Neurospora crassa
1.7.1.3 NADPH
-
 Neurospora crassa