EC Number | Cloned (Comment) | Organism |
---|---|---|
1.11.1.7 | expressed in Escherichia coli BL21(DE3) cells | Escherichia coli |
1.11.2.5 | C-terminal His8-tagged SfmD is overexpressed in Escherichia coli BL21 (DE3) | Streptomyces lavendulae |
2.1.1.304 | - |
Streptomyces lavendulae |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.11.1.7 | C317A | the heme content of the mutant is very low and the mutant loses the ability to hydroxylate 3-methyltyrosine | Escherichia coli |
1.11.1.7 | H191A | the mutant loses the ability to hydroxylate 3-methyltyrosine | Escherichia coli |
1.11.1.7 | H274A | the mutant loses the ability to hydroxylate 3-methyltyrosine | Escherichia coli |
1.11.1.7 | H313A | the heme content of the mutant is very low and the mutant loses the ability to hydroxylate 3-methyltyrosine | Escherichia coli |
1.11.2.5 | H191A | mutant loses ability to hydroxylate 3-methyl-L-tyrosine | Streptomyces lavendulae |
1.11.2.5 | H274A | mutant loses ability to hydroxylate 3-methyl-L-tyrosine | Streptomyces lavendulae |
1.11.2.5 | H313A | mutant loses ability to hydroxylate 3-methyl-L-tyrosine. Heme content of the mutant enzyme is very low, very little heme-Fe binding ability | Streptomyces lavendulae |
1.11.2.5 | H317A | mutant loses ability to hydroxylate 3-methyl-L-tyrosine. Heme content of the mutant enzyme is very low, very little heme-Fe binding ability | Streptomyces lavendulae |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.11.1.7 | 0.64 | - |
3-methyltyrosine | in 100 mM Tris-HCl, pH 9.0, at 25°C | Escherichia coli | |
1.11.1.7 | 1 | - |
L-tyrosine | in 100 mM Tris-HCl, pH 9.0, at 25°C | Escherichia coli | |
1.11.2.5 | 0.63 | - |
3-methyl-L-tyrosine | pH 9.0, 25°C | Streptomyces lavendulae | |
1.11.2.5 | 1 | - |
L-tyrosine | pH 9.0, 25°C | Streptomyces lavendulae |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.11.1.7 | 39800 | - |
x * 39800, SDS-PAGE | Escherichia coli |
1.11.1.7 | 39855 | - |
x * 39855, LC-MS analysis and calculated from amino acid sequence | Escherichia coli |
1.11.2.5 | 39800 | - |
x * 39800, SDS-PAGE | Streptomyces lavendulae |
1.11.2.5 | 39854 | - |
x * 39854, calculated from sequence (without cofactor) | Streptomyces lavendulae |
2.1.1.304 | 41800 | - |
x * 41800, SDS-PAGE | Streptomyces lavendulae |
2.1.1.304 | 42000 | - |
x * 42000, SDS-PAGE | Streptomyces lavendulae |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.11.1.7 | 3-methyltyrosine + H2O2 | Escherichia coli | - |
3-hydroxy-5-methyltyrosine + H2O | - |
? | |
1.11.2.5 | 3-methyl-L-tyrosine + H2O2 + H+ | Streptomyces lavendulae | the enzyme is involved in biosynthesis of saframycin A, a potent antitumor antibiotic that belongs to the tetrahydroisoquinoline family | 3-hydroxy-5-methyl-L-tyrosine + H2O | - |
? | |
1.11.2.5 | 3-methyl-L-tyrosine + H2O2 + H+ | Streptomyces lavendulae NRRL 11002 | the enzyme is involved in biosynthesis of saframycin A, a potent antitumor antibiotic that belongs to the tetrahydroisoquinoline family | 3-hydroxy-5-methyl-L-tyrosine + H2O | - |
? | |
2.1.1.304 | S-adenosyl-L-methionine + L-tyrosine | Streptomyces lavendulae | the enzyme is involved in biosynthesis of saframycin A, a potent antitumor antibiotic that belongs to the tetrahydroisoquinoline family | S-adenosyl-L-homocysteine + 3-methyl-L-tyrosine | - |
? | |
2.1.1.304 | S-adenosyl-L-methionine + L-tyrosine | Streptomyces lavendulae NRRL 11002 | the enzyme is involved in biosynthesis of saframycin A, a potent antitumor antibiotic that belongs to the tetrahydroisoquinoline family | S-adenosyl-L-homocysteine + 3-methyl-L-tyrosine | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.11.1.7 | Escherichia coli | - |
- |
- |
1.11.2.5 | Streptomyces lavendulae | B0CN28 | - |
- |
1.11.2.5 | Streptomyces lavendulae NRRL 11002 | B0CN28 | - |
- |
2.1.1.304 | Streptomyces lavendulae | B0CN31 | - |
- |
2.1.1.304 | Streptomyces lavendulae NRRL 11002 | B0CN31 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.11.1.7 | Ni-NTA affinity column chromatography | Escherichia coli |
1.11.2.5 | - |
Streptomyces lavendulae |
2.1.1.304 | - |
Streptomyces lavendulae |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.11.1.7 | 3-methyltyrosine + H2O2 | - |
Escherichia coli | 3-hydroxy-5-methyltyrosine + H2O | - |
? | |
1.11.1.7 | L-tyrosine + H2O2 | - |
Escherichia coli | ? | - |
? | |
1.11.2.5 | 3-methyl-L-tyrosine + H2O2 + H+ | the enzyme is involved in biosynthesis of saframycin A, a potent antitumor antibiotic that belongs to the tetrahydroisoquinoline family | Streptomyces lavendulae | 3-hydroxy-5-methyl-L-tyrosine + H2O | - |
? | |
1.11.2.5 | 3-methyl-L-tyrosine + H2O2 + H+ | the enzyme catalyzes the regioselective hydroxylation of 3-methyltyrosine to 3-hydroxy-5-methyl-L-tyrosine | Streptomyces lavendulae | 3-hydroxy-5-methyl-L-tyrosine + H2O | - |
? | |
1.11.2.5 | 3-methyl-L-tyrosine + H2O2 + H+ | the enzyme is involved in biosynthesis of saframycin A, a potent antitumor antibiotic that belongs to the tetrahydroisoquinoline family | Streptomyces lavendulae NRRL 11002 | 3-hydroxy-5-methyl-L-tyrosine + H2O | - |
? | |
1.11.2.5 | 3-methyl-L-tyrosine + H2O2 + H+ | the enzyme catalyzes the regioselective hydroxylation of 3-methyltyrosine to 3-hydroxy-5-methyl-L-tyrosine | Streptomyces lavendulae NRRL 11002 | 3-hydroxy-5-methyl-L-tyrosine + H2O | - |
? | |
1.11.2.5 | L-tyrosine + H2O2 + H+ | - |
Streptomyces lavendulae | ? | - |
? | |
1.11.2.5 | L-tyrosine + H2O2 + H+ | - |
Streptomyces lavendulae NRRL 11002 | ? | - |
? | |
2.1.1.304 | S-adenosyl-L-methionine + L-tyrosine | - |
Streptomyces lavendulae | S-adenosyl-L-homocysteine + 3-methyl-L-tyrosine | - |
? | |
2.1.1.304 | S-adenosyl-L-methionine + L-tyrosine | the enzyme is involved in biosynthesis of saframycin A, a potent antitumor antibiotic that belongs to the tetrahydroisoquinoline family | Streptomyces lavendulae | S-adenosyl-L-homocysteine + 3-methyl-L-tyrosine | - |
? | |
2.1.1.304 | S-adenosyl-L-methionine + L-tyrosine | - |
Streptomyces lavendulae NRRL 11002 | S-adenosyl-L-homocysteine + 3-methyl-L-tyrosine | - |
? | |
2.1.1.304 | S-adenosyl-L-methionine + L-tyrosine | the enzyme is involved in biosynthesis of saframycin A, a potent antitumor antibiotic that belongs to the tetrahydroisoquinoline family | Streptomyces lavendulae NRRL 11002 | S-adenosyl-L-homocysteine + 3-methyl-L-tyrosine | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.11.1.7 | ? | x * 39800, SDS-PAGE | Escherichia coli |
1.11.1.7 | ? | x * 39855, LC-MS analysis and calculated from amino acid sequence | Escherichia coli |
1.11.2.5 | ? | x * 39800, SDS-PAGE | Streptomyces lavendulae |
1.11.2.5 | ? | x * 39854, calculated from sequence (without cofactor) | Streptomyces lavendulae |
2.1.1.304 | ? | x * 42000, SDS-PAGE | Streptomyces lavendulae |
2.1.1.304 | ? | x * 41800, SDS-PAGE | Streptomyces lavendulae |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.11.1.7 | SacD | - |
Escherichia coli |
1.11.1.7 | SfmD | - |
Escherichia coli |
1.11.2.5 | 3-methyl-L-tyrosine peroxidase | - |
Streptomyces lavendulae |
1.11.2.5 | 3-methyltyrosine peroxidase | - |
Streptomyces lavendulae |
1.11.2.5 | SacD | - |
Streptomyces lavendulae |
1.11.2.5 | SfmD | - |
Streptomyces lavendulae |
2.1.1.304 | SacF | - |
Streptomyces lavendulae |
2.1.1.304 | SfmM2 | - |
Streptomyces lavendulae |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.11.2.5 | 25 | - |
assay at | Streptomyces lavendulae |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.11.1.7 | 12.3 | - |
L-tyrosine | in 100 mM Tris-HCl, pH 9.0, at 25°C | Escherichia coli | |
1.11.1.7 | 17.8 | - |
3-methyltyrosine | in 100 mM Tris-HCl, pH 9.0, at 25°C | Escherichia coli | |
1.11.2.5 | 0.205 | - |
L-tyrosine | pH 9.0, 25°C | Streptomyces lavendulae | |
1.11.2.5 | 0.476 | - |
3-methyl-L-tyrosine | pH 9.0, 25°C | Streptomyces lavendulae |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.11.1.7 | 9 | - |
- |
Escherichia coli |
1.11.2.5 | 9 | - |
- |
Streptomyces lavendulae |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.11.1.7 | heme | - |
Escherichia coli | |
1.11.2.5 | heme | a heme containing protein. The conserved motif HXXXC is crucial for heme binding. SfmD binds one molecular of heme | Streptomyces lavendulae |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.11.1.7 | metabolism | the enzyme is involved in the biosynthesis of saframycin A | Escherichia coli |
1.11.2.5 | physiological function | the enzyme is involved in biosynthesis of saframycin A, a potent antitumor antibiotic that belongs to the tetrahydroisoquinoline family | Streptomyces lavendulae |
2.1.1.304 | physiological function | the enzyme is involved in biosynthesis of saframycin A, a potent antitumor antibiotic that belongs to the tetrahydroisoquinoline family | Streptomyces lavendulae |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.11.1.7 | 12.2 | - |
L-tyrosine | in 100 mM Tris-HCl, pH 9.0, at 25°C | Escherichia coli | |
1.11.1.7 | 27.8 | - |
3-methyltyrosine | in 100 mM Tris-HCl, pH 9.0, at 25°C | Escherichia coli | |
1.11.2.5 | 0.205 | - |
L-tyrosine | pH 9.0, 25°C | Streptomyces lavendulae | |
1.11.2.5 | 0.76 | - |
3-methyl-L-tyrosine | pH 9.0, 25°C | Streptomyces lavendulae |