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Literature summary extracted from

  • Tang, M.C.; Fu, C.Y.; Tang, G.L.
    Characterization of SfmD as a heme peroxidase that catalyzes the regioselective hydroxylation of 3-methyltyrosine to 3-hydroxy-5-methyltyrosine in saframycin A biosynthesis (2012), J. Biol. Chem., 287, 5112-5121.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.11.1.7 expressed in Escherichia coli BL21(DE3) cells Escherichia coli
1.11.2.5 C-terminal His8-tagged SfmD is overexpressed in Escherichia coli BL21 (DE3) Streptomyces lavendulae
2.1.1.304
-
Streptomyces lavendulae

Protein Variants

EC Number Protein Variants Comment Organism
1.11.1.7 C317A the heme content of the mutant is very low and the mutant loses the ability to hydroxylate 3-methyltyrosine Escherichia coli
1.11.1.7 H191A the mutant loses the ability to hydroxylate 3-methyltyrosine Escherichia coli
1.11.1.7 H274A the mutant loses the ability to hydroxylate 3-methyltyrosine Escherichia coli
1.11.1.7 H313A the heme content of the mutant is very low and the mutant loses the ability to hydroxylate 3-methyltyrosine Escherichia coli
1.11.2.5 H191A mutant loses ability to hydroxylate 3-methyl-L-tyrosine Streptomyces lavendulae
1.11.2.5 H274A mutant loses ability to hydroxylate 3-methyl-L-tyrosine Streptomyces lavendulae
1.11.2.5 H313A mutant loses ability to hydroxylate 3-methyl-L-tyrosine. Heme content of the mutant enzyme is very low, very little heme-Fe binding ability Streptomyces lavendulae
1.11.2.5 H317A mutant loses ability to hydroxylate 3-methyl-L-tyrosine. Heme content of the mutant enzyme is very low, very little heme-Fe binding ability Streptomyces lavendulae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.7 0.64
-
3-methyltyrosine in 100 mM Tris-HCl, pH 9.0, at 25°C Escherichia coli
1.11.1.7 1
-
L-tyrosine in 100 mM Tris-HCl, pH 9.0, at 25°C Escherichia coli
1.11.2.5 0.63
-
3-methyl-L-tyrosine pH 9.0, 25°C Streptomyces lavendulae
1.11.2.5 1
-
L-tyrosine pH 9.0, 25°C Streptomyces lavendulae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.11.1.7 39800
-
x * 39800, SDS-PAGE Escherichia coli
1.11.1.7 39855
-
x * 39855, LC-MS analysis and calculated from amino acid sequence Escherichia coli
1.11.2.5 39800
-
x * 39800, SDS-PAGE Streptomyces lavendulae
1.11.2.5 39854
-
x * 39854, calculated from sequence (without cofactor) Streptomyces lavendulae
2.1.1.304 41800
-
x * 41800, SDS-PAGE Streptomyces lavendulae
2.1.1.304 42000
-
x * 42000, SDS-PAGE Streptomyces lavendulae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.11.1.7 3-methyltyrosine + H2O2 Escherichia coli
-
3-hydroxy-5-methyltyrosine + H2O
-
?
1.11.2.5 3-methyl-L-tyrosine + H2O2 + H+ Streptomyces lavendulae the enzyme is involved in biosynthesis of saframycin A, a potent antitumor antibiotic that belongs to the tetrahydroisoquinoline family 3-hydroxy-5-methyl-L-tyrosine + H2O
-
?
1.11.2.5 3-methyl-L-tyrosine + H2O2 + H+ Streptomyces lavendulae NRRL 11002 the enzyme is involved in biosynthesis of saframycin A, a potent antitumor antibiotic that belongs to the tetrahydroisoquinoline family 3-hydroxy-5-methyl-L-tyrosine + H2O
-
?
2.1.1.304 S-adenosyl-L-methionine + L-tyrosine Streptomyces lavendulae the enzyme is involved in biosynthesis of saframycin A, a potent antitumor antibiotic that belongs to the tetrahydroisoquinoline family S-adenosyl-L-homocysteine + 3-methyl-L-tyrosine
-
?
2.1.1.304 S-adenosyl-L-methionine + L-tyrosine Streptomyces lavendulae NRRL 11002 the enzyme is involved in biosynthesis of saframycin A, a potent antitumor antibiotic that belongs to the tetrahydroisoquinoline family S-adenosyl-L-homocysteine + 3-methyl-L-tyrosine
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.7 Escherichia coli
-
-
-
1.11.2.5 Streptomyces lavendulae B0CN28
-
-
1.11.2.5 Streptomyces lavendulae NRRL 11002 B0CN28
-
-
2.1.1.304 Streptomyces lavendulae B0CN31
-
-
2.1.1.304 Streptomyces lavendulae NRRL 11002 B0CN31
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.7 Ni-NTA affinity column chromatography Escherichia coli
1.11.2.5
-
Streptomyces lavendulae
2.1.1.304
-
Streptomyces lavendulae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.7 3-methyltyrosine + H2O2
-
Escherichia coli 3-hydroxy-5-methyltyrosine + H2O
-
?
1.11.1.7 L-tyrosine + H2O2
-
Escherichia coli ?
-
?
1.11.2.5 3-methyl-L-tyrosine + H2O2 + H+ the enzyme is involved in biosynthesis of saframycin A, a potent antitumor antibiotic that belongs to the tetrahydroisoquinoline family Streptomyces lavendulae 3-hydroxy-5-methyl-L-tyrosine + H2O
-
?
1.11.2.5 3-methyl-L-tyrosine + H2O2 + H+ the enzyme catalyzes the regioselective hydroxylation of 3-methyltyrosine to 3-hydroxy-5-methyl-L-tyrosine Streptomyces lavendulae 3-hydroxy-5-methyl-L-tyrosine + H2O
-
?
1.11.2.5 3-methyl-L-tyrosine + H2O2 + H+ the enzyme is involved in biosynthesis of saframycin A, a potent antitumor antibiotic that belongs to the tetrahydroisoquinoline family Streptomyces lavendulae NRRL 11002 3-hydroxy-5-methyl-L-tyrosine + H2O
-
?
1.11.2.5 3-methyl-L-tyrosine + H2O2 + H+ the enzyme catalyzes the regioselective hydroxylation of 3-methyltyrosine to 3-hydroxy-5-methyl-L-tyrosine Streptomyces lavendulae NRRL 11002 3-hydroxy-5-methyl-L-tyrosine + H2O
-
?
1.11.2.5 L-tyrosine + H2O2 + H+
-
Streptomyces lavendulae ?
-
?
1.11.2.5 L-tyrosine + H2O2 + H+
-
Streptomyces lavendulae NRRL 11002 ?
-
?
2.1.1.304 S-adenosyl-L-methionine + L-tyrosine
-
Streptomyces lavendulae S-adenosyl-L-homocysteine + 3-methyl-L-tyrosine
-
?
2.1.1.304 S-adenosyl-L-methionine + L-tyrosine the enzyme is involved in biosynthesis of saframycin A, a potent antitumor antibiotic that belongs to the tetrahydroisoquinoline family Streptomyces lavendulae S-adenosyl-L-homocysteine + 3-methyl-L-tyrosine
-
?
2.1.1.304 S-adenosyl-L-methionine + L-tyrosine
-
Streptomyces lavendulae NRRL 11002 S-adenosyl-L-homocysteine + 3-methyl-L-tyrosine
-
?
2.1.1.304 S-adenosyl-L-methionine + L-tyrosine the enzyme is involved in biosynthesis of saframycin A, a potent antitumor antibiotic that belongs to the tetrahydroisoquinoline family Streptomyces lavendulae NRRL 11002 S-adenosyl-L-homocysteine + 3-methyl-L-tyrosine
-
?

Subunits

EC Number Subunits Comment Organism
1.11.1.7 ? x * 39800, SDS-PAGE Escherichia coli
1.11.1.7 ? x * 39855, LC-MS analysis and calculated from amino acid sequence Escherichia coli
1.11.2.5 ? x * 39800, SDS-PAGE Streptomyces lavendulae
1.11.2.5 ? x * 39854, calculated from sequence (without cofactor) Streptomyces lavendulae
2.1.1.304 ? x * 42000, SDS-PAGE Streptomyces lavendulae
2.1.1.304 ? x * 41800, SDS-PAGE Streptomyces lavendulae

Synonyms

EC Number Synonyms Comment Organism
1.11.1.7 SacD
-
Escherichia coli
1.11.1.7 SfmD
-
Escherichia coli
1.11.2.5 3-methyl-L-tyrosine peroxidase
-
Streptomyces lavendulae
1.11.2.5 3-methyltyrosine peroxidase
-
Streptomyces lavendulae
1.11.2.5 SacD
-
Streptomyces lavendulae
1.11.2.5 SfmD
-
Streptomyces lavendulae
2.1.1.304 SacF
-
Streptomyces lavendulae
2.1.1.304 SfmM2
-
Streptomyces lavendulae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.11.2.5 25
-
assay at Streptomyces lavendulae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.11.1.7 12.3
-
L-tyrosine in 100 mM Tris-HCl, pH 9.0, at 25°C Escherichia coli
1.11.1.7 17.8
-
3-methyltyrosine in 100 mM Tris-HCl, pH 9.0, at 25°C Escherichia coli
1.11.2.5 0.205
-
L-tyrosine pH 9.0, 25°C Streptomyces lavendulae
1.11.2.5 0.476
-
3-methyl-L-tyrosine pH 9.0, 25°C Streptomyces lavendulae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.1.7 9
-
-
Escherichia coli
1.11.2.5 9
-
-
Streptomyces lavendulae

Cofactor

EC Number Cofactor Comment Organism Structure
1.11.1.7 heme
-
Escherichia coli
1.11.2.5 heme a heme containing protein. The conserved motif HXXXC is crucial for heme binding. SfmD binds one molecular of heme Streptomyces lavendulae

General Information

EC Number General Information Comment Organism
1.11.1.7 metabolism the enzyme is involved in the biosynthesis of saframycin A Escherichia coli
1.11.2.5 physiological function the enzyme is involved in biosynthesis of saframycin A, a potent antitumor antibiotic that belongs to the tetrahydroisoquinoline family Streptomyces lavendulae
2.1.1.304 physiological function the enzyme is involved in biosynthesis of saframycin A, a potent antitumor antibiotic that belongs to the tetrahydroisoquinoline family Streptomyces lavendulae

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.11.1.7 12.2
-
L-tyrosine in 100 mM Tris-HCl, pH 9.0, at 25°C Escherichia coli
1.11.1.7 27.8
-
3-methyltyrosine in 100 mM Tris-HCl, pH 9.0, at 25°C Escherichia coli
1.11.2.5 0.205
-
L-tyrosine pH 9.0, 25°C Streptomyces lavendulae
1.11.2.5 0.76
-
3-methyl-L-tyrosine pH 9.0, 25°C Streptomyces lavendulae