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Literature summary extracted from

  • Kawahara, R.; Saburi, W.; Odaka, R.; Taguchi, H.; Ito, S.; Mori, H.; Matsui, H.
    Metabolic mechanism of mannan in a ruminal bacterium, Ruminococcus albus, involving two mannoside phosphorylases and cellobiose 2-epimerase: discovery of a new carbohydrate phosphorylase, beta-1,4-mannooligosaccharide phosphorylase (2012), J. Biol. Chem., 287, 42389-42399.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.281 expressed in Escherichia coli BL21 Ruminococcus albus
2.4.1.319 expressed in Escherichia coli BL21 Ruminococcus albus
2.4.1.319 expression in Escherichia coli BL21 (DE3) Ruminococcus albus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.281 alpha-D-mannose 1-phosphate competitive; competitive Ruminococcus albus
2.4.1.281 cellobiose
-
 Ruminococcus albus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.281 0.304
-
 4-O-beta-D-mannopyranosyl-D-glucopyranose pH 6.5, 37°C, phosphorolytic reaction, cosubstrate phosphate Ruminococcus albus
2.4.1.281 0.324
-
 alpha-D-mannose 1-phosphate pH 6.5, 37°C, synthetic reaction, cosubstrate D-glucose Ruminococcus albus
2.4.1.281 0.444
-
 phosphate pH 6.5, 37°C, phosphorolytic reaction, cosubstrate 4-O-beta-D-mannopyranosyl-D-glucopyranose Ruminococcus albus
2.4.1.281 5.34
-
 phosphate pH 6.5, 37°C, phosphorolytic reaction, cosubstrate 4-O-beta-D-mannopyranosyl-D-glucopyranose Ruminococcus albus
2.4.1.281 5.8
-
 alpha-D-mannose 1-phosphate pH 6.5, 37°C, synthetic reaction, cosubstrate D-glucose Ruminococcus albus
2.4.1.281 6.14
-
 D-allose apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
2.4.1.281 6.44
-
 3-deoxy-3-fluoro-D-glucose apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
2.4.1.281 7.82
-
 3-O-methyl-D-glucose apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
2.4.1.281 22.2
-
 cellobiose apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
2.4.1.281 27.7
-
 4-O-beta-D-mannopyranosyl-D-glucopyranose pH 6.5, 37°C, phosphorolytic reaction, cosubstrate phosphate Ruminococcus albus
2.4.1.281 29.1
-
 laminaribiose apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
2.4.1.281 38.2
-
 D-glucose pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
2.4.1.281 45.6
-
 D-mannose apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
2.4.1.281 48.7
-
 2-deoxy-2-fluoro-D-glucose apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
2.4.1.281 49.8
-
 sophorose apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
2.4.1.281 49.9
-
 N,N'-diacetylchitobiose apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
2.4.1.281 50.4
-
 maltose apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
2.4.1.281 60.8
-
 gentiobiose apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
2.4.1.281 79.2
-
 1,5-anhydro-D-glucitol apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
2.4.1.281 85.2
-
 methyl beta-D-glucoside apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
2.4.1.281 85.3
-
 methyl alpha-D-glucoside apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
2.4.1.281 90.7
-
 2-deoxy-D-glucose apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
2.4.1.281 107
-
 N-acetyl-D-glucosamine apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
2.4.1.281 119
-
 6-deoxy-D-glucose pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
2.4.1.281 156
-
 6-deoxy-6-fluoro-D-glucose pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
2.4.1.281 172
-
 D-glucose apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
2.4.1.319 3.21
-
 [(1->4)-beta-D-mannosyl]4 pH 6.5, 37°C, cosubstrate: phosphate, phosphorolysis reaction Ruminococcus albus
2.4.1.319 3.21
-
 beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man pH 6.5, 37°C, phosphorolytic reaction, cosubstrate phosphate Ruminococcus albus
2.4.1.319 4.48
-
 [(1->4)-beta-D-mannosyl]6 pH 6.5, 37°C, cosubstrate: alpha-D-mannose 1-phosphate, synthetic reaction Ruminococcus albus
2.4.1.319 4.48
-
 beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
2.4.1.319 4.55
-
 [(1->4)-beta-D-mannosyl]5 pH 6.5, 37°C, cosubstrate: phosphate, phosphorolysis reaction Ruminococcus albus
2.4.1.319 4.55
-
 beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man pH 6.5, 37°C, phosphorolytic reaction, cosubstrate phosphate Ruminococcus albus
2.4.1.319 5.29
-
 [(1->4)-beta-D-mannosyl]5 pH 6.5, 37°C, cosubstrate: alpha-D-mannose 1-phosphate, synthetic reaction Ruminococcus albus
2.4.1.319 5.29
-
 beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
2.4.1.319 7.59
-
 [(1->4)-beta-D-mannosyl]4 pH 6.5, 37°C, cosubstrate: alpha-D-mannose 1-phosphate, synthetic reaction Ruminococcus albus
2.4.1.319 7.59
-
 beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
2.4.1.319 7.94
-
 [(1->4)-beta-D-mannosyl]3 pH 6.5, 37°C, cosubstrate: phosphate, phosphorolysis reaction Ruminococcus albus
2.4.1.319 7.94
-
 beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man pH 6.5, 37°C, phosphorolytic reaction, cosubstrate phosphate Ruminococcus albus
2.4.1.319 13.3
-
 [(1->4)-beta-D-mannosyl]3 pH 6.5, 37°C, cosubstrate: alpha-D-mannose 1-phosphate, synthetic reaction Ruminococcus albus
2.4.1.319 13.3
-
 beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
2.4.1.319 28.4
-
 4-O-beta-D-mannosyl-D-glucose pH 6.5, 37°C, cosubstrate: phosphate, phosphorolysis reaction Ruminococcus albus
2.4.1.319 30.7
-
 [(1->4)-beta-D-mannosyl]2 pH 6.5, 37°C, cosubstrate: alpha-D-mannose 1-phosphate, synthetic reaction Ruminococcus albus
2.4.1.319 30.7
-
 beta-D-mannopyranosyl-(1->4)-D-mannopyranose pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
2.4.1.319 44.5
-
 [(1->4)-beta-D-mannosyl]2 pH 6.5, 37°C, cosubstrate: phosphate, phosphorolysis reaction Ruminococcus albus
2.4.1.319 44.5
-
 beta-D-mannopyranosyl-(1->4)-D-mannopyranose pH 6.5, 37°C, phosphorolytic reaction, cosubstrate phosphate Ruminococcus albus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.281 cytoplasm
-
 Ruminococcus albus 5737
-
2.4.1.319 cytoplasm
-
 Ruminococcus albus 5737
-
2.4.1.319 intracellular
-
 Ruminococcus albus 5622
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.281 37823
-
 6 * 38000, SDS-PAGE, 4 * 37823, amino acid sequence Ruminococcus albus
2.4.1.281 38000
-
 6 * 38000, SDS-PAGE, 4 * 37823, amino acid sequence Ruminococcus albus
2.4.1.281 43646
-
 2 * 45000, SDS-PAGE, 4 * 43646, amino acid sequence Ruminococcus albus
2.4.1.281 45000
-
 2 * 45000, SDS-PAGE, 4 * 43646, amino acid sequence Ruminococcus albus
2.4.1.281 80000
-
 gel filtration Ruminococcus albus
2.4.1.281 209000
-
 gel filtration Ruminococcus albus
2.4.1.319 37823
-
 6 * 38000, SDS-PAGE, 4 * 37823, amino acid sequence Ruminococcus albus
2.4.1.319 38000
-
 6 * 38000, SDS-PAGE Ruminococcus albus
2.4.1.319 38000
-
 6 * 38000, SDS-PAGE, 4 * 37823, amino acid sequence Ruminococcus albus
2.4.1.319 209000
-
 gel filtration Ruminococcus albus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.281 4-O-beta-D-mannopyranosyl-D-glucopyranose + phosphate Ruminococcus albus
-
 D-glucose + alpha-D-mannose 1-phosphate
-
 r
2.4.1.281 4-O-beta-D-mannopyranosyl-D-glucopyranose + phosphate Ruminococcus albus NE1
-
 D-glucose + alpha-D-mannose 1-phosphate
-
 r
2.4.1.281 6-deoxy-6-fluoro-D-glucose + alpha-D-mannose 1-phosphate Ruminococcus albus
-
 4-O-beta-D-mannopyranosyl-6-deoxy-6-fluoro-D-glucopyranose + phosphate
-
 ?
2.4.1.281 6-deoxy-6-fluoro-D-glucose + alpha-D-mannose 1-phosphate Ruminococcus albus NE1
-
 4-O-beta-D-mannopyranosyl-6-deoxy-6-fluoro-D-glucopyranose + phosphate
-
 ?
2.4.1.281 6-deoxy-D-glucose + alpha-D-mannose 1-phosphate Ruminococcus albus
-
 4-O-beta-D-mannopyranosyl-6-deoxy-D-glucopyranose + phosphate
-
 ?
2.4.1.281 6-deoxy-D-glucose + alpha-D-mannose 1-phosphate Ruminococcus albus NE1
-
 4-O-beta-D-mannopyranosyl-6-deoxy-D-glucopyranose + phosphate
-
 ?
2.4.1.281 D-glucose + alpha-D-mannose 1-phosphate Ruminococcus albus
-
 4-O-beta-D-mannopyranosyl-D-glucopyranose + phosphate
-
 r
2.4.1.281 D-glucose + alpha-D-mannose 1-phosphate Ruminococcus albus NE1
-
 4-O-beta-D-mannopyranosyl-D-glucopyranose + phosphate
-
 r
2.4.1.281 laminaribiose + alpha-D-mannose 1-phosphate Ruminococcus albus
-
 ? + phosphate
-
 ?
2.4.1.281 laminaribiose + alpha-D-mannose 1-phosphate Ruminococcus albus NE1
-
 ? + phosphate
-
 ?
2.4.1.319 [(1->4)-beta-D-mannosyl]n + phosphate Ruminococcus albus
-
 [(1->4)-beta-D-mannosyl]n-1 + alpha-D-mannose 1-phosphate
-
 r
2.4.1.319 [(1->4)-beta-D-mannosyl]n + phosphate Ruminococcus albus the enzyme is involved in the metabolic pathway of mannan [(1->4)-beta-D-mannosyl]n-1 + alpha-D-mannose 1-phosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.281 Ruminococcus albus E6UBR9
-
-
2.4.1.281 Ruminococcus albus E6UIS7
-
-
2.4.1.281 Ruminococcus albus NE1 E6UBR9
-
-
2.4.1.281 Ruminococcus albus NE1 E6UIS7
-
-
2.4.1.319 Ruminococcus albus E6UBR9
-
-
2.4.1.319 Ruminococcus albus NE1 E6UBR9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.281
-
 Ruminococcus albus
2.4.1.319
-
 Ruminococcus albus
2.4.1.319 recombinant enzyme Ruminococcus albus

Reaction

EC Number Reaction Comment Organism Reaction ID
2.4.1.281 4-O-beta-D-mannopyranosyl-D-glucopyranose + phosphate = D-glucose + alpha-D-mannose 1-phosphate sequential Bi Bi mechanism Ruminococcus albus
a
2.4.1.319 [(1->4)-beta-D-mannosyl]n + phosphate = [(1->4)-beta-D-mannosyl]n-1 + alpha-D-mannose 1-phosphate n>=3, sequential bi-bi mechanism Ruminococcus albus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.319 2.03
-
 pH 6.5, 37°C Ruminococcus albus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.281 1,5-anhydro-D-glucitol + alpha-D-mannose 1-phosphate
-
 Ruminococcus albus 4-O-beta-D-mannopyranosyl-1,5-anhydro-D-glucitol + phosphate
-
 r
2.4.1.281 1,5-anhydro-D-glucitol + alpha-D-mannose 1-phosphate
-
 Ruminococcus albus NE1 4-O-beta-D-mannopyranosyl-1,5-anhydro-D-glucitol + phosphate
-
 r
2.4.1.281 2-deoxy-2-fluoro-D-glucose + alpha-D-mannose 1-phosphate
-
 Ruminococcus albus 4-O-beta-D-mannopyranosyl-2-deoxy-2-fluoro-D-glucopyranose + phosphate
-
 r
2.4.1.281 2-deoxy-D-glucose + alpha-D-mannose 1-phosphate
-
 Ruminococcus albus 4-O-beta-D-mannopyranosyl-2-deoxy-D-glucopyranose + phosphate
-
 r
2.4.1.281 3-deoxy-3-fluoro-D-glucose + alpha-D-mannose 1-phosphate
-
 Ruminococcus albus 4-O-beta-D-mannopyranosyl-3-deoxy-3-fluoro-D-glucopyranose + phosphate
-
 r
2.4.1.281 3-O-methyl-D-glucose + alpha-D-mannose 1-phosphate
-
 Ruminococcus albus 4-O-beta-D-mannopyranosyl-3-O-methyl-D-glucopyranose + phosphate
-
 r
2.4.1.281 4-O-beta-D-mannopyranosyl-D-glucopyranose + phosphate
-
 Ruminococcus albus D-glucose + alpha-D-mannose 1-phosphate
-
 r
2.4.1.281 4-O-beta-D-mannopyranosyl-D-glucopyranose + phosphate
-
 Ruminococcus albus NE1 D-glucose + alpha-D-mannose 1-phosphate
-
 r
2.4.1.281 6-deoxy-6-fluoro-D-glucose + alpha-D-mannose 1-phosphate
-
 Ruminococcus albus 4-O-beta-D-mannopyranosyl-6-deoxy-6-fluoro-D-glucopyranose + phosphate
-
 ?
2.4.1.281 6-deoxy-6-fluoro-D-glucose + alpha-D-mannose 1-phosphate
-
 Ruminococcus albus NE1 4-O-beta-D-mannopyranosyl-6-deoxy-6-fluoro-D-glucopyranose + phosphate
-
 ?
2.4.1.281 6-deoxy-D-glucose + alpha-D-mannose 1-phosphate
-
 Ruminococcus albus 4-O-beta-D-mannopyranosyl-6-deoxy-D-glucopyranose + phosphate
-
 ?
2.4.1.281 6-deoxy-D-glucose + alpha-D-mannose 1-phosphate
-
 Ruminococcus albus NE1 4-O-beta-D-mannopyranosyl-6-deoxy-D-glucopyranose + phosphate
-
 ?
2.4.1.281 cellobiose + alpha-D-mannose 1-phosphate
-
 Ruminococcus albus ? + phosphate
-
 r
2.4.1.281 D-allose + alpha-D-mannose 1-phosphate
-
 Ruminococcus albus 4-O-beta-D-mannopyranosyl-D-allose + phosphate
-
 r
2.4.1.281 D-glucose + alpha-D-mannose 1-phosphate
-
 Ruminococcus albus 4-O-beta-D-mannopyranosyl-D-glucopyranose + phosphate
-
 r
2.4.1.281 D-glucose + alpha-D-mannose 1-phosphate
-
 Ruminococcus albus NE1 4-O-beta-D-mannopyranosyl-D-glucopyranose + phosphate
-
 r
2.4.1.281 D-mannose + alpha-D-mannose 1-phosphate
-
 Ruminococcus albus 4-O-beta-D-mannopyranosyl-D-mannopyranose + phosphate
-
 r
2.4.1.281 gentiobiose + alpha-D-mannose 1-phosphate
-
 Ruminococcus albus ? + phosphate
-
 r
2.4.1.281 laminaribiose + alpha-D-mannose 1-phosphate
-
 Ruminococcus albus ? + phosphate
-
 r
2.4.1.281 laminaribiose + alpha-D-mannose 1-phosphate
-
 Ruminococcus albus ? + phosphate
-
 ?
2.4.1.281 laminaribiose + alpha-D-mannose 1-phosphate
-
 Ruminococcus albus NE1 ? + phosphate
-
 r
2.4.1.281 laminaribiose + alpha-D-mannose 1-phosphate
-
 Ruminococcus albus NE1 ? + phosphate
-
 ?
2.4.1.281 maltose + alpha-D-mannose 1-phosphate
-
 Ruminococcus albus ? + phosphate
-
 r
2.4.1.281 methyl alpha-D-glucoside + alpha-D-mannose 1-phosphate
-
 Ruminococcus albus methyl [4-O-beta-D-mannopyranosyl]-alpha-D-glucoside + phosphate
-
 r
2.4.1.281 methyl beta-D-glucoside + alpha-D-mannose 1-phosphate
-
 Ruminococcus albus methyl [4-O-beta-D-mannopyranosyl]-beta-D-glucopyranoside + phosphate
-
 r
2.4.1.281 additional information The enzyme also catalyzes the reaction of beta-1,4-mannooligosaccharide phosphorylase Ruminococcus albus ?
-
 ?
2.4.1.281 additional information The enzyme also catalyzes the reaction of beta-1,4-mannooligosaccharide phosphorylase Ruminococcus albus NE1 ?
-
 ?
2.4.1.281 N,N'-diacetylchitobiose + alpha-D-mannose 1-phosphate
-
 Ruminococcus albus ? + phosphate
-
 r
2.4.1.281 N-acetyl-D-glucosamine + alpha-D-mannose 1-phosphate
-
 Ruminococcus albus 4-O-beta-D-mannopyranosyl-N-acetyl-D-glucosamine + phosphate
-
 r
2.4.1.281 sophorose + alpha-D-mannose 1-phosphate
-
 Ruminococcus albus ? + phosphate
-
 r
2.4.1.319 4-O-beta-D-mannosyl-D-glucose + phosphate
-
 Ruminococcus albus D-glucose + alpha-D-mannosyl 1-phosphate
-
 ?
2.4.1.319 beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man + alpha-D-mannose 1-phosphate
-
 Ruminococcus albus beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man + phosphate
-
 r
2.4.1.319 beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man + alpha-D-mannose 1-phosphate
-
 Ruminococcus albus beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man + phosphate
-
 r
2.4.1.319 beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man + phosphate
-
 Ruminococcus albus beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man + alpha-D-mannose 1-phosphate
-
 r
2.4.1.319 beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man + alpha-D-mannose 1-phosphate best substrate for the synthetic reaction Ruminococcus albus beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man + phosphate
-
 r
2.4.1.319 beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man + phosphate
-
 Ruminococcus albus beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man + alpha-D-mannose 1-phosphate
-
 r
2.4.1.319 beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man + alpha-D-mannose 1-phosphate
-
 Ruminococcus albus beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man + phosphate
-
 r
2.4.1.319 beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man + phosphate
-
 Ruminococcus albus beta-D-mannopyranosyl-(1->4)-D-mannopyranose + alpha-D-mannose 1-phosphate
-
 r
2.4.1.319 beta-D-mannopyranosyl-(1->4)-beta-D-mannopyranosyl-(1->4)-D-mannopyranose + phosphate
-
 Ruminococcus albus beta-D-mannopyranosyl-(1->4)-D-mannopyranose + alpha-D-mannose 1-phosphate
-
 r
2.4.1.319 beta-D-mannopyranosyl-(1->4)-D-mannopyranose + alpha-D-mannose 1-phosphate
-
 Ruminococcus albus beta-D-mannopyranosyl-(1->4)-beta-D-mannopyranosyl-(1->4)-D-mannopyranose + phosphate
-
 r
2.4.1.319 beta-D-mannopyranosyl-(1->4)-D-mannopyranose + phosphate
-
 Ruminococcus albus D-mannose + alpha-D-mannose 1-phosphate
-
 r
2.4.1.319 additional information The enzyme also catalyzes the reaction of EC 2.4.1.281, 4-O-beta-D-mannosyl-D-glucose phosphorylase Ruminococcus albus ?
-
 ?
2.4.1.319 [(1->4)-beta-D-mannosyl]2 + alpha-D-mannose 1-phosphate
-
 Ruminococcus albus [(1->4)-beta-D-mannosyl]3 + phosphate
-
 ?
2.4.1.319 [(1->4)-beta-D-mannosyl]2 + phosphate
-
 Ruminococcus albus D-mannose + alpha-D-mannose 1-phosphate
-
 ?
2.4.1.319 [(1->4)-beta-D-mannosyl]3 + alpha-D-mannose 1-phosphate
-
 Ruminococcus albus [(1->4)-beta-D-mannosyl]4 + phosphate
-
 ?
2.4.1.319 [(1->4)-beta-D-mannosyl]3 + phosphate
-
 Ruminococcus albus [(1->4)-beta-D-mannosyl]2 + alpha-D-mannose 1-phosphate
-
 ?
2.4.1.319 [(1->4)-beta-D-mannosyl]4 + alpha-D-mannose 1-phosphate
-
 Ruminococcus albus [(1->4)-beta-D-mannosyl]5 + phosphate
-
 ?
2.4.1.319 [(1->4)-beta-D-mannosyl]4 + phosphate
-
 Ruminococcus albus [(1->4)-beta-D-mannosyl]3 + alpha-D-mannose 1-phosphate
-
 ?
2.4.1.319 [(1->4)-beta-D-mannosyl]5 + alpha-D-mannose 1-phosphate
-
 Ruminococcus albus [(1->4)-beta-D-mannosyl]6 + phosphate
-
 ?
2.4.1.319 [(1->4)-beta-D-mannosyl]5 + phosphate
-
 Ruminococcus albus [(1->4)-beta-D-mannosyl]4 + alpha-D-mannose 1-phosphate
-
 ?
2.4.1.319 [(1->4)-beta-D-mannosyl]6 + alpha-D-mannose 1-phosphate
-
 Ruminococcus albus [(1->4)-beta-D-mannosyl]7 + phosphate
-
 ?
2.4.1.319 [(1->4)-beta-D-mannosyl]6 + phosphate
-
 Ruminococcus albus [(1->4)-beta-D-mannosyl]5 + alpha-D-mannose 1-phosphate
-
 ?
2.4.1.319 [(1->4)-beta-D-mannosyl]n + phosphate
-
 Ruminococcus albus [(1->4)-beta-D-mannosyl]n-1 + alpha-D-mannose 1-phosphate
-
 r
2.4.1.319 [(1->4)-beta-D-mannosyl]n + phosphate the enzyme is involved in the metabolic pathway of mannan Ruminococcus albus [(1->4)-beta-D-mannosyl]n-1 + alpha-D-mannose 1-phosphate
-
 r
2.4.1.319 [(1->4)-beta-D-mannosyl]n + phosphate the enzyme catalyses the reversible phosphorolysis of beta-1,4-mannooligosaccharide with a minimum size of three monomers. It acts on D-glucose derivatives at the C2- and C3-positions, including deoxy- and deoxyfluoro-analogues and epimers, but not on those substituted at the C6-position. It shows high synthetic activity toward the following oligosaccharides: beta-linked glucobioses, maltose, N,N'-diacetylchitobiose, and beta-1,4-mannooligosaccharides. Particularly, beta-1,4-mannooligosaccharides serve as significantly better acceptor substrates for the enzyme than D-glucose. In the phosphorolytic reactions, RaMP2 has weak activity toward beta-1,4-mannobiose but efficiently degrades beta-1,4-mannooligosaccharides longer than beta-1,4-mannobiose. It is thought to catalyze the phosphorolysis of beta-1,4-mannooligosaccharides longer than beta-1,4-mannobiose to produce alpha-D-mannose 1-phosphate and beta-1,4-mannobiose Ruminococcus albus [(1->4)-beta-D-mannosyl]n-1 + alpha-D-mannose 1-phosphate
-
 r
2.4.1.319 [(1->4)-beta-D-mannosyl]n-1 + alpha-D-mannose 1-phosphate the enzyme catalyses the reversible phosphorolysis of beta-1,4-mannooligosaccharide with a minimum size of three monomers. It acts on D-glucose derivatives at the C2- and C3-positions, including deoxy- and deoxyfluoro-analogues and epimers, but not on those substituted at the C6-position. It shows high synthetic activity toward the following oligosaccharides: beta-linked glucobioses, maltose, N,N'-diacetylchitobiose, and beta-1,4-mannooligosaccharides. Particularly, beta-1,4-mannooligosaccharides serve as significantly better acceptor substrates for the enzyme than D-glucose. In the phosphorolytic reactions, RaMP2 has weak activity toward beta-1,4-mannobiose but efficiently degrades beta-1,4-mannooligosaccharides longer than beta-1,4-mannobiose. It is thought to catalyze the phosphorolysis of beta-1,4-mannooligosaccharides longer than beta-1,4-mannobiose to produce alpha-D-mannose 1-phosphate and beta-1,4-mannobiose Ruminococcus albus [(1->4)-beta-D-mannosyl]n + phosphate
-
 r

Subunits

EC Number Subunits Comment Organism
2.4.1.281 homodimer 2 * 45000, SDS-PAGE, 4 * 43646, amino acid sequence Ruminococcus albus
2.4.1.281 homohexamer 6 * 38000, SDS-PAGE, 4 * 37823, amino acid sequence Ruminococcus albus
2.4.1.319 homohexamer 6 * 38000, SDS-PAGE Ruminococcus albus
2.4.1.319 homohexamer 6 * 38000, SDS-PAGE, 4 * 37823, amino acid sequence Ruminococcus albus

Synonyms

EC Number Synonyms Comment Organism
2.4.1.281 RaMp1
-
 Ruminococcus albus
2.4.1.281 RaMp2
-
 Ruminococcus albus
2.4.1.319 RaMp2
-
 Ruminococcus albus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.281 45
-
-
 Ruminococcus albus
2.4.1.281 50
-
-
 Ruminococcus albus
2.4.1.319 37
-
 assay at Ruminococcus albus
2.4.1.319 45
-
-
 Ruminococcus albus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.281 40
-
 stable up to Ruminococcus albus
2.4.1.281 45
-
 stable up to Ruminococcus albus
2.4.1.319 40
-
 stable up to Ruminococcus albus
2.4.1.319 40
-
 stable below Ruminococcus albus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.281 0.667
-
 D-allose apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
2.4.1.281 0.763
-
 3-O-methyl-D-glucose apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
2.4.1.281 0.94
-
 3-deoxy-3-fluoro-D-glucose apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
2.4.1.281 5.07
-
 maltose apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
2.4.1.281 5.12
-
 D-mannose apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
2.4.1.281 5.71
-
 2-deoxy-2-fluoro-D-glucose apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
2.4.1.281 6.34
-
 N,N'-diacetylchitobiose apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
2.4.1.281 11.6
-
 laminaribiose apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
2.4.1.281 11.7
-
 2-deoxy-D-glucose apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
2.4.1.281 13.8
-
 sophorose apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
2.4.1.281 15.8
-
 N-acetyl-D-glucosamine apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
2.4.1.281 17.5
-
 4-O-beta-D-mannopyranosyl-D-glucopyranose pH 6.5, 37°C, phosphorolytic reaction, cosubstrate phosphate Ruminococcus albus
2.4.1.281 20.2
-
 cellobiose apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
2.4.1.281 20.7
-
 1,5-anhydro-D-glucitol apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
2.4.1.281 20.7
-
 methyl alpha-D-glucoside apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
2.4.1.281 26.1
-
 gentiobiose apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
2.4.1.281 34.5
-
 D-glucose apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
2.4.1.281 35.2
-
 methyl beta-D-glucoside apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
2.4.1.281 42
-
 6-deoxy-6-fluoro-D-glucose pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
2.4.1.281 45.1
-
 D-glucose pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
2.4.1.281 83.3
-
 4-O-beta-D-mannopyranosyl-D-glucopyranose pH 6.5, 37°C, phosphorolytic reaction, cosubstrate phosphate Ruminococcus albus
2.4.1.281 177
-
 6-deoxy-D-glucose pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
2.4.1.319 7.06
-
 [(1->4)-beta-D-mannosyl]2 pH 6.5, 37°C, cosubstrate: phosphate, phosphorolysis reaction Ruminococcus albus
2.4.1.319 7.06
-
 beta-D-mannopyranosyl-(1->4)-D-mannopyranose pH 6.5, 37°C, phosphorolytic reaction, cosubstrate phosphate Ruminococcus albus
2.4.1.319 16
-
 [(1->4)-beta-D-mannosyl]6 pH 6.5, 37°C, cosubstrate: alpha-D-mannose 1-phosphate, synthetic reaction Ruminococcus albus
2.4.1.319 16
-
 beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
2.4.1.319 20.5
-
 [(1->4)-beta-D-mannosyl]5 pH 6.5, 37°C, cosubstrate: alpha-D-mannose 1-phosphate, synthetic reaction Ruminococcus albus
2.4.1.319 20.5
-
 beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
2.4.1.319 20.7
-
 4-O-beta-D-mannosyl-D-glucose pH 6.5, 37°C, cosubstrate: phosphate, phosphorolysis reaction Ruminococcus albus
2.4.1.319 27.5
-
 [(1->4)-beta-D-mannosyl]3 pH 6.5, 37°C, cosubstrate: phosphate, phosphorolysis reaction Ruminococcus albus
2.4.1.319 27.5
-
 beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man pH 6.5, 37°C, phosphorolytic reaction, cosubstrate phosphate Ruminococcus albus
2.4.1.319 31.2
-
 [(1->4)-beta-D-mannosyl]4 pH 6.5, 37°C, cosubstrate: alpha-D-mannose 1-phosphate, synthetic reaction Ruminococcus albus
2.4.1.319 31.2
-
 beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
2.4.1.319 31.9
-
 [(1->4)-beta-D-mannosyl]5 pH 6.5, 37°C, cosubstrate: phosphate, phosphorolysis reaction Ruminococcus albus
2.4.1.319 31.9
-
 beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man pH 6.5, 37°C, phosphorolytic reaction, cosubstrate phosphate Ruminococcus albus
2.4.1.319 33.1
-
 [(1->4)-beta-D-mannosyl]4 pH 6.5, 37°C, cosubstrate: phosphate, phosphorolysis reaction Ruminococcus albus
2.4.1.319 33.1
-
 beta-D-Man-(1->4)-beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man pH 6.5, 37°C, phosphorolytic reaction, cosubstrate phosphate Ruminococcus albus
2.4.1.319 34.6
-
 [(1->4)-beta-D-mannosyl]3 pH 6.5, 37°C, cosubstrate: alpha-D-mannose 1-phosphate, synthetic reaction Ruminococcus albus
2.4.1.319 34.6
-
 beta-D-Man-(1->4)-beta-D-Man-(1->4)-D-Man pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
2.4.1.319 45.1
-
 [(1->4)-beta-D-mannosyl]2 pH 6.5, 37°C, cosubstrate: alpha-D-mannose 1-phosphate, synthetic reaction Ruminococcus albus
2.4.1.319 45.1
-
 beta-D-mannopyranosyl-(1->4)-D-mannopyranose pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.281 6.5
-
-
 Ruminococcus albus
2.4.1.319 6.5
-
-
 Ruminococcus albus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.4.1.281 3.5 9.5 4°C, 24 h stable Ruminococcus albus
2.4.1.281 4.5 10.5 4°C, 24 h stable Ruminococcus albus
2.4.1.319 3.5 9.5 stable Ruminococcus albus
2.4.1.319 3.5 9.5 4°C, 24 h stable Ruminococcus albus

General Information

EC Number General Information Comment Organism
2.4.1.319 physiological function the enzyme is involved in the metabolic pathway of mannan Ruminococcus albus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.1.281 0.0976
-
 3-O-methyl-D-glucose apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
2.4.1.281 0.101
-
 maltose apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
2.4.1.281 0.109
-
 D-allose apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
2.4.1.281 0.112
-
 D-mannose apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
2.4.1.281 0.117
-
 2-deoxy-2-fluoro-D-glucose apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
2.4.1.281 0.127
-
 N,N'-diacetylchitobiose apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
2.4.1.281 0.129
-
 2-deoxy-D-glucose apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
2.4.1.281 0.146
-
 3-deoxy-3-fluoro-D-glucose apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
2.4.1.281 0.148
-
 N-acetyl-D-glucosamine apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
2.4.1.281 0.201
-
 D-glucose apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
2.4.1.281 0.236
-
 methyl alpha-D-glucoside apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
2.4.1.281 0.261
-
 1,5-anhydro-D-glucitol apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
2.4.1.281 0.269
-
 6-deoxy-6-fluoro-D-glucose pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
2.4.1.281 0.277
-
 sophorose apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
2.4.1.281 0.399
-
 laminaribiose apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
2.4.1.281 0.413
-
 methyl beta-D-glucoside apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
2.4.1.281 0.429
-
 gentiobiose apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
2.4.1.281 0.91
-
 cellobiose apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
2.4.1.281 1.49
-
 6-deoxy-D-glucose pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate Ruminococcus albus
2.4.1.319 0.159
-
 [(1->4)-beta-D-mannosyl]2 pH 6.5, 37°C, cosubstrate: phosphate, phosphorolysis reaction Ruminococcus albus
2.4.1.319 0.729
-
 4-O-beta-D-mannosyl-D-glucose pH 6.5, 37°C, cosubstrate: phosphate, phosphorolysis reaction Ruminococcus albus
2.4.1.319 1.47
-
 [(1->4)-beta-D-mannosyl]2 pH 6.5, 37°C, cosubstrate: alpha-D-mannose 1-phosphate, synthetic reaction Ruminococcus albus
2.4.1.319 2.6
-
 [(1->4)-beta-D-mannosyl]3 pH 6.5, 37°C, cosubstrate: alpha-D-mannose 1-phosphate, synthetic reaction Ruminococcus albus
2.4.1.319 3.46
-
 [(1->4)-beta-D-mannosyl]3 pH 6.5, 37°C, cosubstrate: phosphate, phosphorolysis reaction Ruminococcus albus
2.4.1.319 3.57
-
 [(1->4)-beta-D-mannosyl]6 pH 6.5, 37°C, cosubstrate: alpha-D-mannose 1-phosphate, synthetic reaction Ruminococcus albus
2.4.1.319 3.87
-
 [(1->4)-beta-D-mannosyl]5 pH 6.5, 37°C, cosubstrate: alpha-D-mannose 1-phosphate, synthetic reaction Ruminococcus albus
2.4.1.319 4.11
-
 [(1->4)-beta-D-mannosyl]4 pH 6.5, 37°C, cosubstrate: alpha-D-mannose 1-phosphate, synthetic reaction Ruminococcus albus
2.4.1.319 7.01
-
 [(1->4)-beta-D-mannosyl]5 pH 6.5, 37°C, cosubstrate: phosphate, phosphorolysis reaction Ruminococcus albus
2.4.1.319 10.3
-
 [(1->4)-beta-D-mannosyl]4 pH 6.5, 37°C, cosubstrate: phosphate, phosphorolysis reaction Ruminococcus albus