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Literature summary extracted from

  • Morales, M.; Mate, M.J.; Romero, A.; Martinez, M.J.; Martinez, A.T.; Ruiz-Duenas, F.J.
    Two oxidation sites for low redox potential substrates: a directed mutagenesis, kinetic, and crystallographic study on Pleurotus eryngii versatile peroxidase (2012), J. Biol. Chem., 287, 41053-41067.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.11.1.16 mutants E140G, P141G, K176G, and E140G/K176G, to 1.6, 2.0, 1.5, 1.7 and 2.35 A resolution, respectively Pleurotus eryngii

Protein Variants

EC Number Protein Variants Comment Organism
1.11.1.16 E140G substitution of bulky residue at the main heme access channel, kinetic analysis Pleurotus eryngii
1.11.1.16 E140G/K176G variant attains catalytic efficiencies for oxidation of 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) at the heme channel similar to those of the exposed tryptophan site W164 Pleurotus eryngii
1.11.1.16 E140G/P141G substitution of bulky residue at the main heme access channel, kinetic analysis Pleurotus eryngii
1.11.1.16 E140G/P141G/K176G variant attains catalytic efficiencies for oxidation of 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) at the heme channel similar to those of the exposed tryptophan site W164 Pleurotus eryngii
1.11.1.16 E140G/W164S/K176G variant attains catalytic efficiencies for oxidation of 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) at the heme channel similar to those of the exposed tryptophan site W164 Pleurotus eryngii
1.11.1.16 F142G substitution of bulky residue at the main heme access channel, kinetic analysis Pleurotus eryngii
1.11.1.16 K176D substitution of bulky residue at the main heme access channel, kinetic analysis Pleurotus eryngii
1.11.1.16 K176G substitution of bulky residue at the main heme access channel, kinetic analysis Pleurotus eryngii
1.11.1.16 K215G substitution of bulky residue at the main heme access channel, kinetic analysis Pleurotus eryngii
1.11.1.16 K215Q substitution of bulky residue at the main heme access channel, kinetic analysis Pleurotus eryngii
1.11.1.16 P141G substitution of bulky residue at the main heme access channel, kinetic analysis Pleurotus eryngii
1.11.1.16 P76G substitution of bulky residue at the main heme access channel, kinetic analysis Pleurotus eryngii
1.11.1.16 W164S loss activity. Residue is responsible for high redox potential substrate oxidation Pleurotus eryngii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.16 0.0023
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant P76G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 0.003
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) wild-type, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 0.004
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant K176D, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 0.0041
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant F142G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 0.0054
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant K176G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 0.0054
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant K215G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 0.0054
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant P141G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 0.0059
-
 4-hydroquinone mutant F142G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 0.008
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant K215Q, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 0.0103
-
 4-hydroquinone mutant P76G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 0.0122
-
 4-hydroquinone mutant K215Q, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 0.013
-
 4-hydroquinone mutant K176D, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 0.0143
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant E140G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 0.0156
-
 4-hydroquinone wild-type, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 0.0174
-
 4-hydroquinone mutant K215G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 0.0189
-
 4-hydroquinone mutant K176G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 0.0194
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant E140G/P141G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 0.0194
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant E140G/P141G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 0.0205
-
 4-hydroquinone mutant E140G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 0.0251
-
 4-hydroquinone mutant E140G/K176G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 0.034
-
 catechol mutant E140G/W164S/K176G, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 0.036
-
 2,6-dimethoxyphenol mutant P76G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 0.0362
-
 4-hydroquinone mutant E140G/P141G/K176G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 0.038
-
 2,6-dimethoxyphenol mutant K176D, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 0.0397
-
 4-hydroquinone mutant E140G/P141G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 0.04
-
 4-hydroquinone mutant P141G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 0.058
-
 2,6-dimethoxyphenol mutant K215G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 0.065
-
 2,6-dimethoxyphenol mutant F142G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 0.066
-
 2,6-dimethoxyphenol mutant E140G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 0.078
-
 2,6-dimethoxyphenol mutant K215Q, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 0.078
-
 2,6-dimethoxyphenol wild-type, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 0.0836
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant K215G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 0.1
-
 2,6-dimethoxyphenol mutant K176G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 0.104
-
 2,6-dimethoxyphenol mutant E140G/P141G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 0.119
-
 2,6-dimethoxyphenol mutant E140G/P141G/K176G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 0.155
-
 2,6-dimethoxyphenol mutant P141G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 0.189
-
 2,6-dimethoxyphenol mutant E140G/K176G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 0.305
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant E140G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 0.38
-
 4-hydroquinone mutant F142G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 0.383
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant K176G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 0.41
-
 4-hydroquinone mutant E140G/P141G/K176G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 0.42
-
 4-hydroquinone mutant K176D, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 0.461
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant P141G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 0.618
-
 4-hydroquinone mutant E140G/K176G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 0.716
-
 4-hydroquinone wild-type, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 0.828
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant F142G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 0.836
-
 4-hydroquinone mutant E140G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 0.884
-
 4-hydroquinone mutant E140G/W164S/K176G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 1
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant W164S, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 1.02
-
 4-hydroquinone mutant K215Q, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 1.026
-
 4-hydroquinone mutant P76G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 1.05
-
 4-hydroquinone mutant K176G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 1.09
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) wild-type, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 1.112
-
 4-hydroquinone mutant K215G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 1.114
-
 4-hydroquinone mutant P141G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 1.18
-
 4-hydroquinone mutant E140G/P141G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 1.66
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant K215Q, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 1.86
-
 catechol mutant K176D, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 1.97
-
 guaiacol mutant E140G, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 2.23
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant K176D, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 2.24
-
 4-hydroquinone mutant W164S, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 2.38
-
 2,6-dimethoxyphenol mutant E140G/P141G/K176G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 2.56
-
 2,6-dimethoxyphenol mutant K215Q, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 2.63
-
 catechol mutant E140G/K176G, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 2.73
-
 guaiacol mutant E140G/K176G, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 2.86
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant P76G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 2.97
-
 2,6-dimethoxyphenol mutant E140G/K176G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 3.33
-
 2,6-dimethoxyphenol mutant E140G/W164S/K176G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 3.68
-
 catechol mutant K176G, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 4.04
-
 catechol mutant K215Q, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 4.26
-
 catechol mutant E140G, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 4.26
-
 catechol mutant K215G, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 4.72
-
 catechol mutant E140G/P141G/K176G, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 4.79
-
 catechol mutant P76G, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 5.04
-
 catechol wild-type, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 5.1
-
 guaiacol mutant E140G/P141G, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 5.11
-
 catechol mutant P141G, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 5.15
-
 catechol mutant F142G, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 5.85
-
 guaiacol mutant E140G/W164S/K176G, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 6.9
-
 guaiacol mutant P141G, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 7.27
-
 guaiacol mutant K176D, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 7.76
-
 catechol mutant W164S, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 10.3
-
 guaiacol mutant K215G, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 10.5
-
 catechol mutant E140G/P141G, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 10.5
-
 2,6-dimethoxyphenol wild-type, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 10.8
-
 guaiacol mutant F142G, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 11.1
-
 guaiacol wild-type, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 11.6
-
 guaiacol mutant K215Q, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 14.2
-
 guaiacol mutant E140G/P141G/K176G, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 16
-
 2,6-dimethoxyphenol mutant E140G/P141G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 16.2
-
 guaiacol mutant K176G, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 16.3
-
 guaiacol mutant P76G, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 36.2
-
 2,6-dimethoxyphenol mutant K176G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 37.4
-
 2,6-dimethoxyphenol mutant E140G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 37.6
-
 2,6-dimethoxyphenol mutant W164S, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 39.8
-
 guaiacol mutant W164S, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 76
-
 2,6-dimethoxyphenol mutant P141G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.16 Pleurotus eryngii O94753 isoform Vpl2
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.16 2 2,6-dimethoxyphenol + 2 H2O2
-
 Pleurotus eryngii coerulignone + 2 H2O
-
 ?
1.11.1.16 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2
-
 Pleurotus eryngii ? + H2O
-
 ?
1.11.1.16 4-hydroquinone + H2O2
-
 Pleurotus eryngii 4-benzoquinone + H2O
-
 ?
1.11.1.16 catechol + H2O2
-
 Pleurotus eryngii 2-benzoquinone + H2O
-
 ?
1.11.1.16 guaiacol + H2O2
-
 Pleurotus eryngii 3,3'-dimethoxy-4,4'-biphenylquinone + H2O
-
 ?
1.11.1.16 additional information presence of two independent catalytic sites for different phenols and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) in native enzyme, characterizedby high and low specificity constants, i.e. Km in the micromolar range and Km in the millimolar range, respcetively Pleurotus eryngii ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.11.1.16 Vpl2
-
 Pleurotus eryngii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.11.1.16 4.6
-
 2,6-dimethoxyphenol mutant E140G/P141G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 4.8
-
 2,6-dimethoxyphenol mutant P141G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 5.6
-
 2,6-dimethoxyphenol wild-type, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 6.2
-
 2,6-dimethoxyphenol mutant P76G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 6.6
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant P76G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 7.2
-
 4-hydroquinone mutant K215G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 7.6
-
 2,6-dimethoxyphenol mutant K215Q, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 7.9
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant K215Q, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 8
-
 4-hydroquinone mutant K176D, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 8
-
 4-hydroquinone mutant P76G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 8
-
 catechol mutant W164S, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 8.1
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) wild-type, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 9.3
-
 2,6-dimethoxyphenol mutant K176G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 9.3
-
 guaiacol mutant P141G, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 9.5
-
 2,6-dimethoxyphenol mutant K215G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 9.71
-
 4-hydroquinone mutant F142G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 9.8
-
 2,6-dimethoxyphenol mutant F142G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 10
-
 2,6-dimethoxyphenol mutant E140G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 10.3
-
 4-hydroquinone wild-type, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 10.6
-
 2,6-dimethoxyphenol mutant K176D, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 10.9
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant F142G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 11.4
-
 4-hydroquinone mutant K215Q, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 11.6
-
 4-hydroquinone mutant K176G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 12.6
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant E140G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 13.2
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant K176G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 13.8
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant K176D, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 13.8
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant K215G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 14.6
-
 4-hydroquinone mutant E140G/P141G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 15.6
-
 4-hydroquinone mutant P141G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 16.1
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant P141G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 16.2
-
 4-hydroquinone mutant E140G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 16.2
-
 guaiacol mutant K215G, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 17.3
-
 2,6-dimethoxyphenol mutant E140G/K176G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 17.5
-
 guaiacol mutant F142G, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 19.2
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant E140G/P141G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 19.2
-
 4-hydroquinone mutant F142G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 20
-
 4-hydroquinone mutant E140G/K176G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 20
-
 4-hydroquinone mutant W164S, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 20.4
-
 2,6-dimethoxyphenol mutant E140G/P141G/K176G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 20.4
-
 4-hydroquinone mutant E140G/P141G/K176G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 22.7
-
 guaiacol wild-type, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 23.8
-
 guaiacol mutant K215Q, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 25.2
-
 4-hydroquinone mutant K176D, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 25.8
-
 4-hydroquinone mutant K215G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 26.2
-
 guaiacol mutant P76G, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 29.8
-
 guaiacol mutant K176D, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 29.8
-
 2,6-dimethoxyphenol wild-type, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 29.9
-
 4-hydroquinone mutant P76G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 30.6
-
 guaiacol mutant W164S, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 33.3
-
 guaiacol mutant K176G, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 34.8
-
 guaiacol mutant E140G, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 35.1
-
 4-hydroquinone wild-type, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 35.2
-
 4-hydroquinone mutant K215Q, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 47.6
-
 2,6-dimethoxyphenol mutant W164S, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 50.2
-
 2,6-dimethoxyphenol mutant E140G/W164S/K176G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 56.6
-
 2,6-dimethoxyphenol mutant E140G/P141G/K176G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 61
-
 2,6-dimethoxyphenol mutant E140G/P141G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 65.5
-
 4-hydroquinone mutant E140G/P141G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 67
-
 2,6-dimethoxyphenol mutant E140G/K176G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 70.6
-
 4-hydroquinone mutant K176G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 72.9
-
 4-hydroquinone mutant E140G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 74.5
-
 2,6-dimethoxyphenol mutant K215Q, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 75
-
 catechol mutant F142G, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 78
-
 catechol mutant P141G, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 80
-
 catechol mutant K176G, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 81
-
 catechol mutant K215Q, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 82.1
-
 4-hydroquinone mutant E140G/K176G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 82.2
-
 4-hydroquinone mutant E140G/P141G/K176G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 85
-
 catechol mutant K215G, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 85.6
-
 4-hydroquinone mutant E140G/W164S/K176G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 93
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant P141G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 94
-
 catechol mutant K176D, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 99
-
 catechol mutant P76G, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 105.6
-
 catechol mutant E140G/P141G, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 105.6
-
 guaiacol mutant E140G/P141G, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 108
-
 4-hydroquinone mutant P141G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 135.7
-
 catechol mutant E140G/P141G/K176G, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 135.7
-
 guaiacol mutant E140G/P141G/K176G, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 141
-
 2,6-dimethoxyphenol mutant P141G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 146
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant P76G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 154
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant F142G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 162
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant E140G/P141G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 164
-
 catechol mutant E140G, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 164.8
-
 catechol mutant E140G/W164S/K176G, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 164.8
-
 guaiacol mutant E140G/W164S/K176G, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 165
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant K215Q, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 185
-
 catechol wild-type, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 185.6
-
 catechol mutant E140G/K176G, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 185.6
-
 guaiacol mutant E140G/K176G, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 186
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) wild-type, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 204
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant K215G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 205
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant W164S, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 216
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant E140G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 223
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant K176D, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 227
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant E140G/W164S/K176G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 231.6
-
 2,6-dimethoxyphenol mutant K176G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 235
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant E140G/P141G/K176G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 259
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant K176G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 293
-
 2,6-dimethoxyphenol mutant E140G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 326
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant E140G/K176G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.11.1.16 0.6
-
 guaiacol mutant E140G/P141G, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 0.7
-
 guaiacol mutant W164S, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 1
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant K215Q, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 1
-
 catechol mutant W164S, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 1.3
-
 guaiacol mutant P141G, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 1.3
-
 2,6-dimethoxyphenol mutant W164S, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 1.4
-
 guaiacol mutant E140G/P141G/K176G, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 1.6
-
 guaiacol mutant F142G, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 1.6
-
 guaiacol mutant K215G, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 1.6
-
 guaiacol mutant P76G, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 1.9
-
 2,6-dimethoxyphenol mutant P141G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 2
-
 guaiacol mutant K176G, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 2
-
 guaiacol mutant K215Q, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 2
-
 guaiacol wild-type, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 2.45
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant K176G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 2.53
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant E140G/P141G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 2.6
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant K215G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 2.8
-
 2,6-dimethoxyphenol wild-type, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 2.9
-
 2,6-dimethoxyphenol mutant K215Q, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 3.48
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant K176D, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 3.8
-
 2,6-dimethoxyphenol mutant E140G/P141G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 4.2
-
 guaiacol mutant K176D, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 6.4
-
 2,6-dimethoxyphenol mutant K176G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 7.8
-
 2,6-dimethoxyphenol mutant E140G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 8.9
-
 4-hydroquinone mutant W164S, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 9.3
-
 guaiacol mutant E140G/W164S/K176G, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 10.1
-
 catechol mutant E140G/P141G, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 14.5
-
 catechol mutant F142G, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 15
-
 catechol mutant P141G, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 15.1
-
 2,6-dimethoxyphenol mutant E140G/W164S/K176G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 17.1
-
 guaiacol mutant E140G/K176G, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 17.6
-
 guaiacol mutant E140G, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 19.9
-
 catechol mutant K215G, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 20.1
-
 catechol mutant K215Q, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 20.7
-
 catechol mutant P76G, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 21.6
-
 catechol mutant K176G, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 22.6
-
 2,6-dimethoxyphenol mutant E140G/K176G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 23.8
-
 2,6-dimethoxyphenol mutant E140G/P141G/K176G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 25.3
-
 4-hydroquinone mutant K215G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 28.8
-
 catechol mutant E140G/P141G/K176G, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 29.2
-
 4-hydroquinone mutant P76G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 31
-
 2,6-dimethoxyphenol mutant P141G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 31.4
-
 4-hydroquinone mutant K215Q, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 36.7
-
 catechol wild-type, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 38.4
-
 catechol mutant E140G, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 44.6
-
 2,6-dimethoxyphenol mutant E140G/P141G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 48.4
-
 catechol mutant E140G/W164S/K176G, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 49.1
-
 4-hydroquinone wild-type, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 50.6
-
 4-hydroquinone mutant F142G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 50.9
-
 catechol mutant K176D, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 51
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant P76G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 55.6
-
 4-hydroquinone mutant E140G/P141G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 59.9
-
 4-hydroquinone mutant K176D, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 67
-
 4-hydroquinone mutant K176G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 70.7
-
 catechol mutant E140G/K176G, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 71
-
 2,6-dimethoxyphenol wild-type, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 87.2
-
 4-hydroquinone mutant E140G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 91.3
-
 2,6-dimethoxyphenol mutant E140G/K176G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 93
-
 2,6-dimethoxyphenol mutant K176G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 96.8
-
 4-hydroquinone mutant E140G/W164S/K176G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 97
-
 2,6-dimethoxyphenol mutant K215Q, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 97
-
 4-hydroquinone mutant P141G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 99
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant K215Q, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 100
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant K176D, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 132.8
-
 4-hydroquinone mutant E140G/K176G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 152
-
 2,6-dimethoxyphenol mutant F142G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 153
-
 2,6-dimethoxyphenol mutant E140G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 165
-
 2,6-dimethoxyphenol mutant K215G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 171
-
 2,6-dimethoxyphenol mutant E140G/P141G/K176G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 171
-
 2,6-dimethoxyphenol mutant P76G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 171
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) wild-type, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 185
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant F142G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 200
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant W164S, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 201
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant P141G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 203.2
-
 4-hydroquinone mutant E140G/P141G/K176G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 244
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant K215G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 283
-
 2,6-dimethoxyphenol mutant K176D, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 368
-
 4-hydroquinone mutant E140G/P141G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 387
-
 4-hydroquinone mutant P141G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 565
-
 4-hydroquinone mutant E140G/P141G/K176G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 600
-
 4-hydroquinone mutant K176D, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 600
-
 4-hydroquinone mutant K215G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 616
-
 4-hydroquinone mutant K176G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 656
-
 4-hydroquinone wild-type, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 659
-
 4-hydroquinone mutant K215Q, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 676
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant K176G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 710
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant E140G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 789
-
 4-hydroquinone mutant E140G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 800
-
 4-hydroquinone mutant E140G/K176G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 800
-
 4-hydroquinone mutant P76G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 882
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant E140G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 1120
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant E140G/P141G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 1600
-
 4-hydroquinone mutant F142G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 2640
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant F142G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 2700
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) wild-type, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 2830
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant P76G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 3010
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant P141G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 4090
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant E140G/W164S/K176G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 5670
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant E140G/P141G/K176G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 5680
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant E140G/K176G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii