EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
7.2.1.1 | G140A | subunit NqrB. Site-directed mutagenesis, the mutant shows highly reduced activity and reduced inhibition by 2-n-heptyl-4-hydroxyquinoline N-oxide compared to the wild-type enzyme | Vibrio cholerae serotype O1 |
7.2.1.1 | G140A | sodium-pumping activity of the mutant is not affected under partial turnover conditions. Mutation affects exclusively the binding of ubiquinone | Vibrio cholerae serotype O1 |
7.2.1.1 | G140L | subunit NqrB, site-directed mutagenesis. The mutant shows highly reduced activity compared to the wild-type enzyme and no inhibition by 2-n-heptyl-4-hydroxyquinoline N-oxide in contrast to the wild-type enzyme | Vibrio cholerae serotype O1 |
7.2.1.1 | G140L | mutant shows nonsaturating behavior with up to 50 microM ubiquinone | Vibrio cholerae serotype O1 |
7.2.1.1 | G141A | subunit NqrB, site-directed mutagenesis. The mutant shows reduced activity compared to the wild-type enzyme and no inhibition by 2-n-heptyl-4-hydroxyquinoline N-oxide in contrast to the wild-type enzyme | Vibrio cholerae serotype O1 |
7.2.1.1 | G141A | overall structure of the enzyme is not disturbed by the mutation. Mutation specifically affects the ubiquinone binding site. 6fold increase in Km value | Vibrio cholerae serotype O1 |
7.2.1.1 | G141L | 3fold increase in Km value | Vibrio cholerae serotype O1 |
7.2.1.1 | G141L | subunit NqrB, site-directed mutagenesis. The mutant shows reduced activity compared to the wild-type enzyme and no inhibition by 2-n-heptyl-4-hydroxyquinoline N-oxide in contrast to the wild-type enzyme | Vibrio cholerae serotype O1 |
7.2.1.1 | G141V | site-directed mutagenesis, the mutant shows reduced activity and reduced inhibition by 2-n-heptyl-4-hydroxyquinoline N-oxide compared to the wild-type enzyme | Vibrio cholerae serotype O1 |
7.2.1.1 | G141V | 9fold increase in Km value | Vibrio cholerae serotype O1 |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
7.2.1.1 | 2-n-heptyl-4-hydroxyquinoline N-oxide | - |
Vibrio cholerae serotype O1 |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
7.2.1.1 | additional information | - |
additional information | kinetics | Vibrio cholerae serotype O1 | |
7.2.1.1 | 0.0025 | - |
ubiquinone-1 | wild-type, pH 8.0, temperature not specified in the publication | Vibrio cholerae serotype O1 | |
7.2.1.1 | 0.0025 | - |
ubiquinone | wild-type enzyme, pH 8.0, temperature not specified in the publication | Vibrio cholerae serotype O1 | |
7.2.1.1 | 0.0088 | - |
ubiquinone | NqrB, mutant G141L, pH 8.0, temperature not specified in the publication | Vibrio cholerae serotype O1 | |
7.2.1.1 | 0.0088 | - |
ubiquinone-1 | NqrB, mutant G141L, pH 8.0, temperature not specified in the publication | Vibrio cholerae serotype O1 | |
7.2.1.1 | 0.015 | - |
ubiquinone | NqrB, mutant G141A, pH 8.0, temperature not specified in the publication | Vibrio cholerae serotype O1 | |
7.2.1.1 | 0.015 | - |
ubiquinone-1 | NqrB, mutant G141A, pH 8.0, temperature not specified in the publication | Vibrio cholerae serotype O1 | |
7.2.1.1 | 0.0234 | - |
ubiquinone | NqrB, mutant G141V, pH 8.0, temperature not specified in the publication | Vibrio cholerae serotype O1 | |
7.2.1.1 | 0.0234 | - |
ubiquinone-1 | NqrB, mutant G141V, pH 8.0, temperature not specified in the publication | Vibrio cholerae serotype O1 | |
7.2.1.1 | 0.1 | - |
ubiquinone | above, mutant NqrB-G140A, pH 8.0, temperature not specified in the publication | Vibrio cholerae serotype O1 | |
7.2.1.1 | 0.1 | - |
ubiquinone-1 | mutant G140A, pH 8.0, temperature not specified in the publication | Vibrio cholerae serotype O1 | |
7.2.1.1 | 0.1 | - |
ubiquinone-1 | mutant G140L, pH 8.0, temperature not specified in the publication | Vibrio cholerae serotype O1 |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
7.2.1.1 | NADH + H+ + ubiquinone + n Na+/in | Vibrio cholerae serotype O1 | - |
NAD+ + ubiquinol + n Na+/out | - |
? | |
7.2.1.1 | NADH + H+ + ubiquinone + n Na+/in | Vibrio cholerae serotype O1 ATCC 39541 | - |
NAD+ + ubiquinol + n Na+/out | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
7.2.1.1 | Vibrio cholerae serotype O1 | A5F5X1 and A5F5X0 and A5F5Y7 and A5F5Y6 and A5F5Y3 and A5F5Y4 | A5F5X1: subunit NqrA, A5F5X0: subunit NqrB, A5F5Y7: subunit NqrC, A5F5Y6: subunit NqrD; A5F5Y3: subunit NqrE, A5F5Y4: subunit NqrF. The enzyme consists of six subunits encoded by the NQR operon. | - |
7.2.1.1 | Vibrio cholerae serotype O1 ATCC 39541 | A5F5X1 and A5F5X0 and A5F5Y7 and A5F5Y6 and A5F5Y3 and A5F5Y4 | A5F5X1: subunit NqrA, A5F5X0: subunit NqrB, A5F5Y7: subunit NqrC, A5F5Y6: subunit NqrD; A5F5Y3: subunit NqrE, A5F5Y4: subunit NqrF. The enzyme consists of six subunits encoded by the NQR operon. | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
7.2.1.1 | NADH + H+ + ubiquinone + n Na+/in | - |
Vibrio cholerae serotype O1 | NAD+ + ubiquinol + n Na+/out | - |
? | |
7.2.1.1 | NADH + H+ + ubiquinone + n Na+/in | NqrB-G140 and -G141 are critical for the binding and reaction of Na+-NQR with its electron acceptor, ubiquinone | Vibrio cholerae serotype O1 | NAD+ + ubiquinol + n Na+/out | - |
? | |
7.2.1.1 | NADH + H+ + ubiquinone + n Na+/in | - |
Vibrio cholerae serotype O1 ATCC 39541 | NAD+ + ubiquinol + n Na+/out | - |
? | |
7.2.1.1 | NADH + H+ + ubiquinone + n Na+/in | NqrB-G140 and -G141 are critical for the binding and reaction of Na+-NQR with its electron acceptor, ubiquinone | Vibrio cholerae serotype O1 ATCC 39541 | NAD+ + ubiquinol + n Na+/out | - |
? | |
7.2.1.1 | NADH + H+ + ubiquinone-1 + n Na+/in | - |
Vibrio cholerae serotype O1 | NAD+ + ubiquinol-1 + n Na+/out | - |
? | |
7.2.1.1 | NADH + H+ + ubiquinone-1 + n Na+/in | - |
Vibrio cholerae serotype O1 ATCC 39541 | NAD+ + ubiquinol-1 + n Na+/out | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
7.2.1.1 | Na+-NQR | - |
Vibrio cholerae serotype O1 |
7.2.1.1 | Na+-pumping NADH:quinone oxidoreductase | - |
Vibrio cholerae serotype O1 |
7.2.1.1 | NqrB | - |
Vibrio cholerae serotype O1 |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
7.2.1.1 | 200 | - |
ubiquinone | mutant G140A, pH 8.0, temperature not specified in the publication | Vibrio cholerae serotype O1 | |
7.2.1.1 | 200 | - |
ubiquinone-1 | mutant G140A, pH 8.0, temperature not specified in the publication | Vibrio cholerae serotype O1 | |
7.2.1.1 | 200 | - |
ubiquinone-1 | mutant G140L, pH 8.0, temperature not specified in the publication | Vibrio cholerae serotype O1 | |
7.2.1.1 | 388 | - |
ubiquinone | mutant G141V, pH 8.0, temperature not specified in the publication | Vibrio cholerae serotype O1 | |
7.2.1.1 | 388 | - |
ubiquinone-1 | mutant G141V, pH 8.0, temperature not specified in the publication | Vibrio cholerae serotype O1 | |
7.2.1.1 | 425 | - |
ubiquinone | mutant G141A, pH 8.0, temperature not specified in the publication | Vibrio cholerae serotype O1 | |
7.2.1.1 | 425 | - |
ubiquinone-1 | mutant G141A, pH 8.0, temperature not specified in the publication | Vibrio cholerae serotype O1 | |
7.2.1.1 | 463 | - |
ubiquinone | mutant G141L, pH 8.0, temperature not specified in the publication | Vibrio cholerae serotype O1 | |
7.2.1.1 | 463 | - |
ubiquinone-1 | mutant G141L, pH 8.0, temperature not specified in the publication | Vibrio cholerae serotype O1 | |
7.2.1.1 | 525 | - |
ubiquinone-1 | wild-type, pH 8.0, temperature not specified in the publication | Vibrio cholerae serotype O1 | |
7.2.1.1 | 525 | - |
ubiquinone | wild-type enzyme, pH 8.0, temperature not specified in the publication | Vibrio cholerae serotype O1 |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
7.2.1.1 | 8 | - |
assay at | Vibrio cholerae serotype O1 |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
7.2.1.1 | FAD | non-covalently bound in the NqrF subunit | Vibrio cholerae serotype O1 | |
7.2.1.1 | FMN | two covalently bound in subunits NqrB und NqrC | Vibrio cholerae serotype O1 | |
7.2.1.1 | NADH | - |
Vibrio cholerae serotype O1 | |
7.2.1.1 | riboflavin | one non-covalently bound in the subunit NqrB | Vibrio cholerae serotype O1 |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
7.2.1.1 | additional information | - |
additional information | inhibition kinetics | Vibrio cholerae serotype O1 | |
7.2.1.1 | 0.0005 | - |
2-n-heptyl-4-hydroxyquinoline N-oxide | wild-type enzyme, pH 8.0, temperature not specified in the publication | Vibrio cholerae serotype O1 | |
7.2.1.1 | 0.0005 | - |
2-n-heptyl-4-hydroxyquinoline N-oxide | wild-type, pH 8.0, temperature not specified in the publication | Vibrio cholerae serotype O1 | |
7.2.1.1 | 0.0012 | - |
2-n-heptyl-4-hydroxyquinoline N-oxide | mutant G141V, pH 8.0, temperature not specified in the publication | Vibrio cholerae serotype O1 | |
7.2.1.1 | 0.0075 | - |
2-n-heptyl-4-hydroxyquinoline N-oxide | above, mutant G140A, pH 8.0, temperature not specified in the publication | Vibrio cholerae serotype O1 | |
7.2.1.1 | 0.0075 | - |
2-n-heptyl-4-hydroxyquinoline N-oxide | mutant G140A, pH 8.0, temperature not specified in the publication | Vibrio cholerae serotype O1 |
EC Number | General Information | Comment | Organism |
---|---|---|---|
7.2.1.1 | malfunction | glycine 140 and glycine 141 of the NqrB subunit in the functional binding of ubiquinone. Mutations at these residues alter the affinity of the enzyme for ubiquinol. Mutations in residue NqrBG140 almost completely abolished the electron transfer to ubiquinone | Vibrio cholerae serotype O1 |
7.2.1.1 | metabolism | the enzyme is the main entrance for electrons into the respiratory chain of many marine and pathogenic bacteria. The enzyme accepts electrons from NADH and donates them to ubiquinone, and the free energy released by this redox reaction is used to create an electrochemical gradient of sodium across the cell membrane | Vibrio cholerae serotype O1 |
7.2.1.1 | additional information | subunit NqrB residues G140 and G141 are critical for the binding and reaction of Na+-NQR with its electron acceptor, ubiquinone | Vibrio cholerae serotype O1 |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
7.2.1.1 | 525 | - |
ubiquinone-1 | wild-type, pH 8.0, temperature not specified in the publication | Vibrio cholerae serotype O1 | |
7.2.1.1 | 525 | - |
ubiquinone-1 | mutant G140A, pH 8.0, temperature not specified in the publication | Vibrio cholerae serotype O1 | |
7.2.1.1 | 525 | - |
ubiquinone-1 | mutant G140L, pH 8.0, temperature not specified in the publication | Vibrio cholerae serotype O1 | |
7.2.1.1 | 525 | - |
ubiquinone-1 | mutant G141A, pH 8.0, temperature not specified in the publication | Vibrio cholerae serotype O1 | |
7.2.1.1 | 525 | - |
ubiquinone-1 | mutant G141L, pH 8.0, temperature not specified in the publication | Vibrio cholerae serotype O1 | |
7.2.1.1 | 525 | - |
ubiquinone-1 | mutant G141V, pH 8.0, temperature not specified in the publication | Vibrio cholerae serotype O1 |