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Literature summary extracted from

  • Yamauchi, Y.; Hasegawa, A.; Taninaka, A.; Mizutani, M.; Sugimoto, Y.
    NADPH-dependent reductases involved in the detoxification of reactive carbonyls in plants (2011), J. Biol. Chem., 286, 6999-7009.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.74 overexpressed in Escherichia coli as a GST-fusion protein Arabidopsis thaliana
1.3.1.74 overexpressed in Escherichia coli as a GST-fusion protein Cucumis sativus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.1.74 0.01
-
 NADPH pH 7.0, 25°C Arabidopsis thaliana
1.3.1.74 0.023
-
 4-hexen-3-one pH 7.0, 25°C Cucumis sativus
1.3.1.74 0.053
-
 NADH pH 7.0, 25°C Arabidopsis thaliana
1.3.1.74 0.12
-
 4-hexen-3-one pH 7.0, 25°C Arabidopsis thaliana
1.3.1.74 0.13
-
 3-buten-2-one pH 7.0, 25°C Arabidopsis thaliana
1.3.1.74 0.185
-
 NADPH pH 7.0, 25°C Cucumis sativus
1.3.1.74 0.29
-
 1-penten-3-one pH 7.0, 25°C Arabidopsis thaliana
1.3.1.74 0.58
-
 1-penten-3-one pH 7.0, 25°C Cucumis sativus
1.3.1.74 0.63
-
 3-penten-2-one pH 7.0, 25°C Cucumis sativus
1.3.1.74 1.8
-
 crotonaldehyde pH 7.0, 25°C Arabidopsis thaliana
1.3.1.74 2.88
-
 3-buten-2-one pH 7.0, 25°C Cucumis sativus
1.3.1.74 3.5
-
 propenal pH 7.0, 25°C Arabidopsis thaliana
1.3.1.74 4.64
-
 NADH pH 7.0, 25°C Cucumis sativus
1.3.1.74 13.6
-
 propenal pH 7.0, 25°C Cucumis sativus
1.3.1.74 29.8
-
 crotonaldehyde pH 7.0, 25°C Cucumis sativus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.3.1.74 chloroplast
-
 Arabidopsis thaliana 9507
-
1.3.1.74 chloroplast
-
 Cucumis sativus 9507
-
1.3.1.74 cytosol
-
 Cucumis sativus 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.1.74 42000
-
 SDS-PAGE Arabidopsis thaliana
1.3.1.74 42000
-
 SDS-PAGE Cucumis sativus

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.74 Arabidopsis thaliana
-
-
-
1.3.1.74 Cucumis sativus E2S0A5 chloroplast variant
-
1.3.1.74 Cucumis sativus E2S0A6 cytosolic variant
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.74 using anion exchange chromatography, hydroxyapatite, phenyl-Sepharose, and gel filtration Arabidopsis thaliana
1.3.1.74 using anion exchange chromatography, hydroxyapatite, phenyl-Sepharose, and gel filtration Cucumis sativus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.3.1.74 leaf
-
 Arabidopsis thaliana
-
1.3.1.74 leaf
-
 Cucumis sativus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.74 1-penten-3-one + NADH
-
 Arabidopsis thaliana ? + NAD+
-
 ?
1.3.1.74 1-penten-3-one + NADH
-
 Cucumis sativus ? + NAD+
-
 ?
1.3.1.74 1-penten-3-one + NADH
-
 Cucumis sativus pentan-3-one + NAD+
-
 ?
1.3.1.74 1-penten-3-one + NADPH
-
 Cucumis sativus ? + NADP+
-
 ?
1.3.1.74 1-penten-3-one + NADPH
-
 Arabidopsis thaliana pentan-3-one + NADP+
-
 ?
1.3.1.74 1-penten-3-one + NADPH
-
 Cucumis sativus pentan-3-one + NADP+
-
 ?
1.3.1.74 3-buten-2-one + NADH
-
 Arabidopsis thaliana 2-butanone + NAD+
-
 ?
1.3.1.74 3-buten-2-one + NADH
-
 Cucumis sativus 2-butanone + NAD+
-
 ?
1.3.1.74 3-buten-2-one + NADPH
-
 Arabidopsis thaliana 2-butanone + NADP+
-
 ?
1.3.1.74 3-buten-2-one + NADPH
-
 Cucumis sativus 2-butanone + NADP+
-
 ?
1.3.1.74 3-penten-2-one + NADH
-
 Cucumis sativus 2-pentanone + NAD+
-
 ?
1.3.1.74 3-penten-2-one + NADPH
-
 Cucumis sativus 2-pentanone + NADP+
-
 ?
1.3.1.74 4-hexen-3-one + NADH
-
 Arabidopsis thaliana hexan-3-one + NAD+
-
 ?
1.3.1.74 4-hexen-3-one + NADH
-
 Cucumis sativus hexan-3-one + NAD+
-
 ?
1.3.1.74 4-hexen-3-one + NADPH
-
 Arabidopsis thaliana hexan-3-one + NADP+
-
 ?
1.3.1.74 4-hexen-3-one + NADPH
-
 Cucumis sativus hexan-3-one + NADP+
-
 ?
1.3.1.74 crotonaldehyde + NADH
-
 Arabidopsis thaliana butanal + NAD+
-
 ?
1.3.1.74 crotonaldehyde + NADH
-
 Cucumis sativus butanal + NAD+
-
 ?
1.3.1.74 crotonaldehyde + NADPH
-
 Arabidopsis thaliana butanal + NADP+
-
 ?
1.3.1.74 crotonaldehyde + NADPH
-
 Cucumis sativus butanal + NADP+
-
 ?
1.3.1.74 propenal + NADH
-
 Arabidopsis thaliana propanal + NAD+
-
 ?
1.3.1.74 propenal + NADH
-
 Cucumis sativus propanal + NAD+
-
 ?
1.3.1.74 propenal + NADPH
-
 Arabidopsis thaliana propanal + NADP+
-
 ?
1.3.1.74 propenal + NADPH
-
 Cucumis sativus propanal + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.1.74 monomer
-
 Arabidopsis thaliana
1.3.1.74 monomer
-
 Cucumis sativus

Synonyms

EC Number Synonyms Comment Organism
1.3.1.74 alkenal/one oxidoreductase
-
 Arabidopsis thaliana
1.3.1.74 alkenal/one oxidoreductase
-
 Cucumis sativus
1.3.1.74 AtAOR
-
 Arabidopsis thaliana
1.3.1.74 CsAOR
-
 Cucumis sativus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3.1.74 1.3
-
 crotonaldehyde pH 7.0, 25°C Arabidopsis thaliana
1.3.1.74 3.3
-
 4-hexen-3-one pH 7.0, 25°C Arabidopsis thaliana
1.3.1.74 3.5
-
 3-buten-2-one pH 7.0, 25°C Arabidopsis thaliana
1.3.1.74 4
-
 1-penten-3-one pH 7.0, 25°C Arabidopsis thaliana
1.3.1.74 22
-
 3-penten-2-one pH 7.0, 25°C Cucumis sativus
1.3.1.74 22
-
 propenal pH 7.0, 25°C Arabidopsis thaliana
1.3.1.74 23
-
 NADPH pH 7.0, 25°C Arabidopsis thaliana
1.3.1.74 23
-
 NADH pH 7.0, 25°C Arabidopsis thaliana
1.3.1.74 42
-
 crotonaldehyde pH 7.0, 25°C Cucumis sativus
1.3.1.74 61
-
 1-penten-3-one pH 7.0, 25°C Cucumis sativus
1.3.1.74 63
-
 propenal pH 7.0, 25°C Cucumis sativus
1.3.1.74 68
-
 4-hexen-3-one pH 7.0, 25°C Cucumis sativus
1.3.1.74 74
-
 3-buten-2-one pH 7.0, 25°C Cucumis sativus
1.3.1.74 75
-
 NADPH pH 7.0, 25°C Cucumis sativus
1.3.1.74 75
-
 NADH pH 7.0, 25°C Cucumis sativus

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.74 NADH enzyme operates 30times faster in the presence of NADPH than in the presence of NADH Arabidopsis thaliana
1.3.1.74 NADH enzyme operates 30times faster in the presence of NADPH than in the presence of NADH Cucumis sativus
1.3.1.74 NADPH enzyme operates 30times faster in the presence of NADPH than in the presence of NADH Arabidopsis thaliana
1.3.1.74 NADPH enzyme operates 30times faster in the presence of NADPH than in the presence of NADH Cucumis sativus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.3.1.74 4.63
-
 propenal pH 7.0, 25°C Cucumis sativus
1.3.1.74 6.28
-
 propenal pH 7.0, 25°C Arabidopsis thaliana
1.3.1.74 13.8
-
 1-penten-3-one pH 7.0, 25°C Arabidopsis thaliana
1.3.1.74 16.2
-
 NADH pH 7.0, 25°C Cucumis sativus
1.3.1.74 25.7
-
 3-buten-2-one pH 7.0, 25°C Cucumis sativus
1.3.1.74 26.9
-
 3-buten-2-one pH 7.0, 25°C Arabidopsis thaliana
1.3.1.74 27.5
-
 4-hexen-3-one pH 7.0, 25°C Arabidopsis thaliana
1.3.1.74 34.9
-
 3-penten-2-one pH 7.0, 25°C Cucumis sativus
1.3.1.74 105
-
 1-penten-3-one pH 7.0, 25°C Cucumis sativus
1.3.1.74 406
-
 NADPH pH 7.0, 25°C Cucumis sativus
1.3.1.74 434
-
 NADH pH 7.0, 25°C Arabidopsis thaliana
1.3.1.74 2300
-
 NADPH pH 7.0, 25°C Arabidopsis thaliana
1.3.1.74 2960
-
 4-hexen-3-one pH 7.0, 25°C Cucumis sativus