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Literature summary extracted from

  • Fujita, K.; Sakamoto, S.; Ono, Y.; Wakao, M.; Suda, Y.; Kitahara, K.; Suganuma, T.
    Molecular cloning and characterization of a beta-L-arabinobiosidase in Bifidobacterium longum that belongs to a novel glycoside hydrolase family (2011), J. Biol. Chem., 286, 5143-5150.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.187 expressed in Escherichia coli BL21(lambdaDE3) cells Bifidobacterium longum

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.187 EDTA 1 mM EDTA decreases the enzymatic activity to 1.4% Bifidobacterium longum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.187 0.0107
-
4-O-(beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranosyl)-(2S,4S)-4-hydroxyproline in 50 mM sodium acetate (pH 6.0), at 37°C Bifidobacterium longum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.187 Ca2+ 1 mM Ca2+ increases the enzymatic activity by 43.8% Bifidobacterium longum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.187 156797
-
x * 156797, calculated from amino acid sequence Bifidobacterium longum
3.2.1.187 157000
-
x * 157000, SDS-PAGE Bifidobacterium longum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.187 4-O-(beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranosyl)-(2S,4S)-4-hydroxyproline + H2O Bifidobacterium longum specific substrate 4-O-(beta-L-arabinofuranosyl)-(2S,4S)-4-hydroxyproline + beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranose
-
?
3.2.1.187 4-O-(beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranosyl)-(2S,4S)-4-hydroxyproline + H2O Bifidobacterium longum JCM 1217 specific substrate 4-O-(beta-L-arabinofuranosyl)-(2S,4S)-4-hydroxyproline + beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranose
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.187 Bifidobacterium longum
-
-
-
3.2.1.187 Bifidobacterium longum JCM 1217
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.187 TALON metal affinity resin column chromatography Bifidobacterium longum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.187 4-O-(beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranosyl)-(2S,4S)-4-hydroxyproline + H2O specific substrate Bifidobacterium longum 4-O-(beta-L-arabinofuranosyl)-(2S,4S)-4-hydroxyproline + beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranose
-
?
3.2.1.187 4-O-(beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranosyl)-(2S,4S)-4-hydroxyproline + H2O specific substrate Bifidobacterium longum JCM 1217 4-O-(beta-L-arabinofuranosyl)-(2S,4S)-4-hydroxyproline + beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranose
-
?
3.2.1.187 arabinan + H2O
-
Bifidobacterium longum ?
-
?
3.2.1.187 arabinan + H2O
-
Bifidobacterium longum JCM 1217 ?
-
?
3.2.1.187 carrot extension + H2O
-
Bifidobacterium longum ?
-
?
3.2.1.187 carrot extension + H2O
-
Bifidobacterium longum JCM 1217 ?
-
?
3.2.1.187 debranched arabinan + H2O
-
Bifidobacterium longum ?
-
?
3.2.1.187 debranched arabinan + H2O
-
Bifidobacterium longum JCM 1217 ?
-
?
3.2.1.187 additional information no activity with 4-nitrophenyl alpha-L-arabinofuranoside, 4-nitrophenyl alpha-L-arabinopyranoside, 4-nitrophenyl beta-L-arabinopyranoside, arabinoxylan, rhamnogalacturonan, 4-O-(beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranosyl)-(2S,4S)-4-hydroxyproline and 4-O-(beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranosyl)-(1->2)-(beta-L-arabinofuranosyl)-(1->2)-(beta-L-arabinofuranosyl)-(2S,4S)-4-hydroxyproline Bifidobacterium longum ?
-
?
3.2.1.187 additional information no activity with 4-nitrophenyl alpha-L-arabinofuranoside, 4-nitrophenyl alpha-L-arabinopyranoside, 4-nitrophenyl beta-L-arabinopyranoside, arabinoxylan, rhamnogalacturonan, 4-O-(beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranosyl)-(2S,4S)-4-hydroxyproline and 4-O-(beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranosyl)-(1->2)-(beta-L-arabinofuranosyl)-(1->2)-(beta-L-arabinofuranosyl)-(2S,4S)-4-hydroxyproline Bifidobacterium longum JCM 1217 ?
-
?
3.2.1.187 potato lectin + H2O
-
Bifidobacterium longum ?
-
?

Subunits

EC Number Subunits Comment Organism
3.2.1.187 ? x * 157000, SDS-PAGE Bifidobacterium longum
3.2.1.187 ? x * 156797, calculated from amino acid sequence Bifidobacterium longum

Synonyms

EC Number Synonyms Comment Organism
3.2.1.187 beta-L-arabinobiosidase
-
Bifidobacterium longum
3.2.1.187 hypBA2
-
Bifidobacterium longum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.187 30
-
-
Bifidobacterium longum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.187 2.7
-
4-O-(beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranosyl)-(2S,4S)-4-hydroxyproline in 50 mM sodium acetate (pH 6.0), at 37°C Bifidobacterium longum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.187 5.5 6
-
Bifidobacterium longum