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Literature summary extracted from

  • Friedman, E.; Wang, H.; Jiang, K.; Perovic, I.; Deshpande, A.; Pochapsky, T.; Temple, B.; Hicks, S.; Harden, T.; Jones, A.
    Acireductone dioxygenase 1 (ARD1) is an effector of the heterotrimeric G protein beta subunit in Arabidopsis (2011), J. Biol. Chem., 286, 30107-30118.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.13.11.54 Arabidopsis thaliana G protein beta possible mechanism for Arabidopsis thaliana G protein beta activation of acireductone dioxygenase 1 activity, overview Arabidopsis thaliana

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.54 transient expression of GFP-acireductone dioxygenase 1 in Arabidopsis thaliana protoplasts. The coding regions of ARD1–4 are cloned into pDEST17TM and pDEST15TM containing an N-terminal poly-His tag and an N-terminal GST tag, respectively, and are expressed in Escherichia coli strain Rosetta BL21 Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
1.13.11.54 additional information usage of three tDNA insertion mutant alleles, ard1-1 (SALK_119327), ard1-2 (GABI_595C04), and ard1-3 (SALK_034308). Arabidopsis thaliana

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.54 Fe2+ dependent on, acireductone dioxygenase 1 is an active metalloenzyme, Fe2+ is active site bound Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.54 1,2-dihydroxy-5-(methylthio)pent-1-en-3-one + O2 Arabidopsis thaliana
-
 4-(methylthio)-2-oxobutanoate + formate
-
 ?
1.13.11.54 1,2-dihydroxy-5-(methylthio)pent-1-en-3-one + O2 Arabidopsis thaliana Columbia-0
-
 4-(methylthio)-2-oxobutanoate + formate
-
 ?
1.13.11.54 additional information Arabidopsis thaliana acireductone dioxygenase 1 primarily performs the on-pathway reaction with bound Fe2+ as metal cofactor. Acireductone dioxygenase 1 physically interacts with its regulator Arabidopsis thaliana G protein beta, identification of a potential Arabidopsis thaliana G protein beta-acireductone dioxygenase 1 interface, overview ?
-
 ?
1.13.11.54 additional information Arabidopsis thaliana Columbia-0 acireductone dioxygenase 1 primarily performs the on-pathway reaction with bound Fe2+ as metal cofactor. Acireductone dioxygenase 1 physically interacts with its regulator Arabidopsis thaliana G protein beta, identification of a potential Arabidopsis thaliana G protein beta-acireductone dioxygenase 1 interface, overview ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.54 Arabidopsis thaliana Q0WSS4
-
-
1.13.11.54 Arabidopsis thaliana Columbia-0 Q0WSS4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.54 recombinant His-tagged acireductone dioxygenase 1 and GST-tagged acireductone dioxygenase 1 by nickel affinity and glutathione affinity chromatographies, respectively Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.54 1,2-dihydroxy-5-(methylthio)pent-1-en-3-one + O2
-
 Arabidopsis thaliana 4-(methylthio)-2-oxobutanoate + formate
-
 ?
1.13.11.54 1,2-dihydroxy-5-(methylthio)pent-1-en-3-one + O2
-
 Arabidopsis thaliana Columbia-0 4-(methylthio)-2-oxobutanoate + formate
-
 ?
1.13.11.54 additional information acireductone dioxygenase 1 primarily performs the on-pathway reaction with bound Fe2+ as metal cofactor. Acireductone dioxygenase 1 physically interacts with its regulator Arabidopsis thaliana G protein beta, identification of a potential Arabidopsis thaliana G protein beta-acireductone dioxygenase 1 interface, overview Arabidopsis thaliana ?
-
 ?
1.13.11.54 additional information acireductone dioxygenase 1 primarily performs the on-pathway reaction with bound Fe2+ as metal cofactor. Acireductone dioxygenase 1 physically interacts with its regulator Arabidopsis thaliana G protein beta, identification of a potential Arabidopsis thaliana G protein beta-acireductone dioxygenase 1 interface, overview Arabidopsis thaliana Columbia-0 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.54 More homology modeling of acireductone dioxygenase 1, overview Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
1.13.11.54 acireductone dioxygenase 1
-
 Arabidopsis thaliana
1.13.11.54 ARD1
-
 Arabidopsis thaliana

General Information

EC Number General Information Comment Organism
1.13.11.54 additional information identification of potential effectors in the Arabidopsis thaliana G protein beta signaling pathway by an activation tagging approach to randomly create dominant suppressors of agb1-2, overview. Homology modeling of acireductone dioxygenase 1, overview Arabidopsis thaliana
1.13.11.54 physiological function one function of acireductone dioxygenases is to operate in the methionine salvage pathway. Acireductone dioxygenase 1 physically interacts with Arabidopsis thaliana G protein beta, and acireductone dioxygenase enzymatic activity is stimulated by Arabidopsis thaliana G protein beta in vitro. Overexpression of acireductone dioxygenase 1 suppresses the 2-day-old etiolated phenotype of agb1-2. Acireductone dioxygenase 1 and Arabidopsis thaliana G protein beta both control embryonic hypocotyl length by modulating cell division. They also may contribute to the production of ethylene, a product of the methionine salvage pathway. Arabidopsis thaliana G protein beta regulates acireductone dioxygenase 1 activity to control cell division, possible mechanism for Arabidopsis thaliana G protein beta activation of acireductone dioxygenase 1 activity, overview Arabidopsis thaliana