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Literature summary extracted from
- Structural and functional evolution of isopropylmalate dehydrogenases in the leucine and glucosinolate pathways of Arabidopsis thaliana (2011), J. Biol. Chem., 286, 28794-28801.
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 1.1.1.85 | to 2.25 A resolution. Modeling of 3-isopropylmalate binding in the isoform IPMDH2 active site. Results suggest that substitution of one active site residue may lead to altered substrate specificity and metabolic function | Arabidopsis thaliana |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 1.1.1.85 | F137L | mutation to corresponding residue of isoform IMPDH2, reduces activity toward 3-(2'-methylthio)-ethylmalate by 200fold, but enhances catalytic efficiency with 3-isopropylmalate to levels observed with isoform IPMDH2 | Arabidopsis thaliana |
| 1.1.1.85 | L133F | mutation to corresponding residue of isoform IMPDH1, enhances catalytic efficiency with 3-(2'-methylthio)ethylmalate about100fold and reduces activity for 3-isopropylmalate | Arabidopsis thaliana |
| 1.1.1.85 | L134F | mutation to corresponding residue of isoform IMPDH1, reduces activity toward 3-(2'-methylthio)-ethylmalate, but enhances catalytic efficiency with 3-isopropylmalate | Arabidopsis thaliana |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.85 | 0.0092 | - |
(2R,3S)-3-isopropylmalate | wild-type, pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana |  |
| 1.1.1.85 | 0.0109 | - |
(2R,3S)-3-isopropylmalate | wild-type, pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana | |
| 1.1.1.85 | 0.011 | - |
(2R,3S)-3-isopropylmalate | mutant F137L, pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana | |
| 1.1.1.85 | 0.025 | - |
(2R,3S)-3-isopropylmalate | wild-type, pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana | |
| 1.1.1.85 | 0.028 | - |
(2R,3S)-3-isopropylmalate | mutant L134F, pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana | |
| 1.1.1.85 | 0.0303 | - |
(2R,3S)-3-isopropylmalate | mutant L133F, pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana | |
| 1.1.1.85 | 0.045 | - |
3-(2'-methylthio)ethylmalate | wild-type, pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana | |
| 1.1.1.85 | 0.077 | - |
3-(2'-methylthio)ethylmalate | mutant L133F, pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana | |
| 1.1.1.85 | 0.103 | - |
3-(2'-methylthio)ethylmalate | mutant L134F, pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana | |
| 1.1.1.85 | 0.323 | - |
3-(2'-methylthio)ethylmalate | mutant F137L, pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana | |
| 1.1.1.85 | 0.436 | - |
3-(2'-methylthio)ethylmalate | wild-type, pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana | |
| 1.1.1.85 | 0.502 | - |
3-(2'-methylthio)ethylmalate | wild-type, pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.1.85 | Arabidopsis thaliana | P93832 | isoform IPMDH2 | - |
| 1.1.1.85 | Arabidopsis thaliana | Q9FMT1 | isoform IPMDH3 | - |
| 1.1.1.85 | Arabidopsis thaliana | Q9SA14 | isoform IPMDH1 | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.85 | (2R,3S)-3-isopropylmalate + NAD+ | - |
Arabidopsis thaliana | (2S)-2-isopropyl-3-oxosuccinate + NADH + H+ | - |
? | |
| 1.1.1.85 | 3-(2'-methylthio)ethylmalate + NAD+ | - |
Arabidopsis thaliana | ? + NADH + H+ | - |
? | |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.85 | 0.016 | - |
3-(2'-methylthio)ethylmalate | wild-type, pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana | |
| 1.1.1.85 | 0.0167 | - |
3-(2'-methylthio)ethylmalate | wild-type, pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana | |
| 1.1.1.85 | 0.033 | - |
3-(2'-methylthio)ethylmalate | mutant F137L, pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana | |
| 1.1.1.85 | 0.27 | - |
3-(2'-methylthio)ethylmalate | mutant L134F, pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana | |
| 1.1.1.85 | 0.367 | - |
3-(2'-methylthio)ethylmalate | mutant L133F, pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana | |
| 1.1.1.85 | 0.62 | - |
(2R,3S)-3-isopropylmalate | mutant L133F, pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana | |
| 1.1.1.85 | 0.622 | - |
(2R,3S)-3-isopropylmalate | wild-type, pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana | |
| 1.1.1.85 | 0.73 | - |
(2R,3S)-3-isopropylmalate | mutant L134F, pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana | |
| 1.1.1.85 | 0.85 | - |
3-(2'-methylthio)ethylmalate | wild-type, pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana | |
| 1.1.1.85 | 3.83 | - |
(2R,3S)-3-isopropylmalate | mutant F137L, pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana | |
| 1.1.1.85 | 6.22 | - |
(2R,3S)-3-isopropylmalate | wild-type, pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana | |
| 1.1.1.85 | 9.05 | - |
(2R,3S)-3-isopropylmalate | wild-type, pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana | |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.85 | 0.038 | - |
3-(2'-methylthio)ethylmalate | wild-type, pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana | |
| 1.1.1.85 | 0.1 | - |
3-(2'-methylthio)ethylmalate | mutant F137L, pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana | |
| 1.1.1.85 | 0.33 | - |
3-(2'-methylthio)ethylmalate | wild-type, pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana | |
| 1.1.1.85 | 2.59 | - |
3-(2'-methylthio)ethylmalate | mutant L134F, pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana | |
| 1.1.1.85 | 4.76 | - |
3-(2'-methylthio)ethylmalate | mutant L133F, pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana | |
| 1.1.1.85 | 18.77 | - |
3-(2'-methylthio)ethylmalate | wild-type, pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana | |
| 1.1.1.85 | 20.3 | - |
(2R,3S)-3-isopropylmalate | mutant L133F, pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana | |
| 1.1.1.85 | 24.47 | - |
(2R,3S)-3-isopropylmalate | wild-type, pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana | |
| 1.1.1.85 | 25.7 | - |
(2R,3S)-3-isopropylmalate | mutant L134F, pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana | |
| 1.1.1.85 | 336.3 | - |
(2R,3S)-3-isopropylmalate | mutant F137L, pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana | |
| 1.1.1.85 | 570 | - |
(2R,3S)-3-isopropylmalate | wild-type, pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana | |
| 1.1.1.85 | 983.7 | - |
(2R,3S)-3-isopropylmalate | wild-type, pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana | |
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