BRENDA - Enzyme Database

The conserved Rieske oxygenase DAF-36/Neverland is a novel cholesterol-metabolizing enzyme

Yoshiyama-Yanagawa, T.; Enya, S.; Shimada-Niwa, Y.; Yaguchi, S.; Haramoto, Y.; Matsuya, T.; Shiomi, K.; Sasakura, Y.; Takahashi, S.; Asashima, M.; Kataoka, H.; Niwa, R.; J. Biol. Chem. 286, 25756-25762 (2011)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.3.1.21
expression in S2 cell
Bombyx mori
1.3.1.21
expression in S2 cell
Caenorhabditis elegans
1.14.19.21
expressed in Drosophila melanogaster S2 cells
Bombyx mori
1.14.19.21
expressed in Drosophila melanogaster S2 cells
Caenorhabditis elegans
1.14.19.21
expressed in Drosophila melanogaster S2 cells
Danio rerio
1.14.19.21
expressed in Drosophila melanogaster S2 cells
Hemicentrotus pulcherrimus
1.14.19.21
expressed in Drosophila melanogaster S2 cells
Xenopus laevis
Engineering
EC Number
Amino acid exchange
Commentary
Organism
1.3.1.21
C122A
mutation within Rieske [2Fe-2S]-motif, complete loss of activity
Caenorhabditis elegans
1.3.1.21
D234A
mutation within non-heme Fe(II) binding motif, complete loss of activity
Caenorhabditis elegans
1.3.1.21
H190A
mutation within Rieske [2Fe-2S]-motif, complete loss of activity
Bombyx mori
1.3.1.21
H282A
mutation within non-heme Fe(II) binding motif, complete loss of activity
Bombyx mori
1.14.19.21
C122A
inactive
Caenorhabditis elegans
1.14.19.21
C178A
inactive
Xenopus laevis
1.14.19.21
D234A
inactive
Caenorhabditis elegans
1.14.19.21
H190A
inactive
Bombyx mori
1.14.19.21
H282A
inactive
Bombyx mori
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.3.1.21
microsome
-
Bombyx mori
-
-
1.3.1.21
microsome
-
Caenorhabditis elegans
-
-
1.14.19.21
microsome
-
Bombyx mori
-
-
1.14.19.21
microsome
-
Caenorhabditis elegans
-
-
1.14.19.21
microsome
-
Ciona intestinalis
-
-
1.14.19.21
microsome
-
Danio rerio
-
-
1.14.19.21
microsome
-
Hemicentrotus pulcherrimus
-
-
1.14.19.21
microsome
-
Xenopus laevis
-
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.19.21
cholesterol + O2 + NADH + H+
Bombyx mori
-
cholesta-5,7-dien-3beta-ol + NAD+ + H2O
-
-
?
1.14.19.21
cholesterol + O2 + NADH + H+
Caenorhabditis elegans
-
cholesta-5,7-dien-3beta-ol + NADP+ + H2O
-
-
?
1.14.19.21
cholesterol + O2 + NADH + H+
Xenopus laevis
-
cholesta-5,7-dien-3beta-ol + NADP+ + H2O
-
-
?
1.14.19.21
cholesterol + O2 + NADH + H+
Danio rerio
-
cholesta-5,7-dien-3beta-ol + NADP+ + H2O
-
-
?
1.14.19.21
cholesterol + O2 + NADH + H+
Ciona intestinalis
-
cholesta-5,7-dien-3beta-ol + NADP+ + H2O
-
-
?
1.14.19.21
cholesterol + O2 + NADH + H+
Hemicentrotus pulcherrimus
-
cholesta-5,7-dien-3beta-ol + NADP+ + H2O
-
-
?
1.14.19.21
cholesterol + O2 + NADPH + H+
Caenorhabditis elegans
-
cholesta-5,7-dien-3beta-ol + NADP+ + H2O
-
-
?
1.14.19.21
cholesterol + O2 + NADPH + H+
Bombyx mori
-
cholesta-5,7-dien-3beta-ol + NADP+ + H2O
-
-
?
1.14.19.21
cholesterol + O2 + NADPH + H+
Xenopus laevis
-
cholesta-5,7-dien-3beta-ol + NADP+ + H2O
-
-
?
1.14.19.21
cholesterol + O2 + NADPH + H+
Danio rerio
-
cholesta-5,7-dien-3beta-ol + NADP+ + H2O
-
-
?
1.14.19.21
cholesterol + O2 + NADPH + H+
Ciona intestinalis
-
cholesta-5,7-dien-3beta-ol + NADP+ + H2O
-
-
?
1.14.19.21
cholesterol + O2 + NADPH + H+
Hemicentrotus pulcherrimus
-
cholesta-5,7-dien-3beta-ol + NADP+ + H2O
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.3.1.21
Bombyx mori
Q1JUZ2
-
-
1.3.1.21
Caenorhabditis elegans
Q17938
-
-
1.14.19.21
Bombyx mori
Q1JUZ2
-
-
1.14.19.21
Caenorhabditis elegans
Q17938
-
-
1.14.19.21
Ciona intestinalis
F7J186
-
-
1.14.19.21
Danio rerio
Q6DHJ3
-
-
1.14.19.21
Hemicentrotus pulcherrimus
F7J188
-
-
1.14.19.21
Xenopus laevis
F7J184
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.3.1.21
cholesterol + O2 + NAD(P)H + H+
-
725431
Caenorhabditis elegans
cholesta-5,7-dien-3beta-ol + NAD(P)+ + 2 H2O
-
-
-
?
1.3.1.21
cholesterol + O2 + NAD(P)H + H+
-
725431
Bombyx mori
cholesta-5,7-dien-3beta-ol + NAD(P)+ + 2 H2O
-
-
-
?
1.14.19.21
cholesterol + O2 + NADH + H+
-
725431
Bombyx mori
cholesta-5,7-dien-3beta-ol + NAD+ + H2O
-
-
-
?
1.14.19.21
cholesterol + O2 + NADH + H+
-
725431
Caenorhabditis elegans
cholesta-5,7-dien-3beta-ol + NADP+ + H2O
-
-
-
?
1.14.19.21
cholesterol + O2 + NADH + H+
-
725431
Xenopus laevis
cholesta-5,7-dien-3beta-ol + NADP+ + H2O
-
-
-
?
1.14.19.21
cholesterol + O2 + NADH + H+
-
725431
Danio rerio
cholesta-5,7-dien-3beta-ol + NADP+ + H2O
-
-
-
?
1.14.19.21
cholesterol + O2 + NADH + H+
-
725431
Ciona intestinalis
cholesta-5,7-dien-3beta-ol + NADP+ + H2O
-
-
-
?
1.14.19.21
cholesterol + O2 + NADH + H+
-
725431
Hemicentrotus pulcherrimus
cholesta-5,7-dien-3beta-ol + NADP+ + H2O
-
-
-
?
1.14.19.21
cholesterol + O2 + NADPH + H+
-
725431
Caenorhabditis elegans
cholesta-5,7-dien-3beta-ol + NADP+ + H2O
-
-
-
?
1.14.19.21
cholesterol + O2 + NADPH + H+
-
725431
Bombyx mori
cholesta-5,7-dien-3beta-ol + NADP+ + H2O
-
-
-
?
1.14.19.21
cholesterol + O2 + NADPH + H+
-
725431
Xenopus laevis
cholesta-5,7-dien-3beta-ol + NADP+ + H2O
-
-
-
?
1.14.19.21
cholesterol + O2 + NADPH + H+
-
725431
Danio rerio
cholesta-5,7-dien-3beta-ol + NADP+ + H2O
-
-
-
?
1.14.19.21
cholesterol + O2 + NADPH + H+
-
725431
Ciona intestinalis
cholesta-5,7-dien-3beta-ol + NADP+ + H2O
-
-
-
?
1.14.19.21
cholesterol + O2 + NADPH + H+
-
725431
Hemicentrotus pulcherrimus
cholesta-5,7-dien-3beta-ol + NADP+ + H2O
-
-
-
?
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.14.19.21
NADH
-
Bombyx mori
1.14.19.21
NADH
-
Caenorhabditis elegans
1.14.19.21
NADH
-
Ciona intestinalis
1.14.19.21
NADH
-
Danio rerio
1.14.19.21
NADH
-
Hemicentrotus pulcherrimus
1.14.19.21
NADH
-
Xenopus laevis
1.14.19.21
NADPH
-
Bombyx mori
1.14.19.21
NADPH
-
Caenorhabditis elegans
1.14.19.21
NADPH
-
Ciona intestinalis
1.14.19.21
NADPH
-
Danio rerio
1.14.19.21
NADPH
-
Hemicentrotus pulcherrimus
1.14.19.21
NADPH
-
Xenopus laevis
1.14.19.21
[2Fe-2S]-center
-
Bombyx mori
1.14.19.21
[2Fe-2S]-center
-
Caenorhabditis elegans
1.14.19.21
[2Fe-2S]-center
-
Ciona intestinalis
1.14.19.21
[2Fe-2S]-center
-
Danio rerio
1.14.19.21
[2Fe-2S]-center
-
Hemicentrotus pulcherrimus
1.14.19.21
[2Fe-2S]-center
-
Xenopus laevis
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.3.1.21
expression in S2 cell
Bombyx mori
1.3.1.21
expression in S2 cell
Caenorhabditis elegans
1.14.19.21
expressed in Drosophila melanogaster S2 cells
Bombyx mori
1.14.19.21
expressed in Drosophila melanogaster S2 cells
Caenorhabditis elegans
1.14.19.21
expressed in Drosophila melanogaster S2 cells
Danio rerio
1.14.19.21
expressed in Drosophila melanogaster S2 cells
Hemicentrotus pulcherrimus
1.14.19.21
expressed in Drosophila melanogaster S2 cells
Xenopus laevis
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.14.19.21
NADH
-
Bombyx mori
1.14.19.21
NADH
-
Caenorhabditis elegans
1.14.19.21
NADH
-
Ciona intestinalis
1.14.19.21
NADH
-
Danio rerio
1.14.19.21
NADH
-
Hemicentrotus pulcherrimus
1.14.19.21
NADH
-
Xenopus laevis
1.14.19.21
NADPH
-
Bombyx mori
1.14.19.21
NADPH
-
Caenorhabditis elegans
1.14.19.21
NADPH
-
Ciona intestinalis
1.14.19.21
NADPH
-
Danio rerio
1.14.19.21
NADPH
-
Hemicentrotus pulcherrimus
1.14.19.21
NADPH
-
Xenopus laevis
1.14.19.21
[2Fe-2S]-center
-
Bombyx mori
1.14.19.21
[2Fe-2S]-center
-
Caenorhabditis elegans
1.14.19.21
[2Fe-2S]-center
-
Ciona intestinalis
1.14.19.21
[2Fe-2S]-center
-
Danio rerio
1.14.19.21
[2Fe-2S]-center
-
Hemicentrotus pulcherrimus
1.14.19.21
[2Fe-2S]-center
-
Xenopus laevis
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
1.3.1.21
C122A
mutation within Rieske [2Fe-2S]-motif, complete loss of activity
Caenorhabditis elegans
1.3.1.21
D234A
mutation within non-heme Fe(II) binding motif, complete loss of activity
Caenorhabditis elegans
1.3.1.21
H190A
mutation within Rieske [2Fe-2S]-motif, complete loss of activity
Bombyx mori
1.3.1.21
H282A
mutation within non-heme Fe(II) binding motif, complete loss of activity
Bombyx mori
1.14.19.21
C122A
inactive
Caenorhabditis elegans
1.14.19.21
C178A
inactive
Xenopus laevis
1.14.19.21
D234A
inactive
Caenorhabditis elegans
1.14.19.21
H190A
inactive
Bombyx mori
1.14.19.21
H282A
inactive
Bombyx mori
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.3.1.21
microsome
-
Bombyx mori
-
-
1.3.1.21
microsome
-
Caenorhabditis elegans
-
-
1.14.19.21
microsome
-
Bombyx mori
-
-
1.14.19.21
microsome
-
Caenorhabditis elegans
-
-
1.14.19.21
microsome
-
Ciona intestinalis
-
-
1.14.19.21
microsome
-
Danio rerio
-
-
1.14.19.21
microsome
-
Hemicentrotus pulcherrimus
-
-
1.14.19.21
microsome
-
Xenopus laevis
-
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.19.21
cholesterol + O2 + NADH + H+
Bombyx mori
-
cholesta-5,7-dien-3beta-ol + NAD+ + H2O
-
-
?
1.14.19.21
cholesterol + O2 + NADH + H+
Caenorhabditis elegans
-
cholesta-5,7-dien-3beta-ol + NADP+ + H2O
-
-
?
1.14.19.21
cholesterol + O2 + NADH + H+
Xenopus laevis
-
cholesta-5,7-dien-3beta-ol + NADP+ + H2O
-
-
?
1.14.19.21
cholesterol + O2 + NADH + H+
Danio rerio
-
cholesta-5,7-dien-3beta-ol + NADP+ + H2O
-
-
?
1.14.19.21
cholesterol + O2 + NADH + H+
Ciona intestinalis
-
cholesta-5,7-dien-3beta-ol + NADP+ + H2O
-
-
?
1.14.19.21
cholesterol + O2 + NADH + H+
Hemicentrotus pulcherrimus
-
cholesta-5,7-dien-3beta-ol + NADP+ + H2O
-
-
?
1.14.19.21
cholesterol + O2 + NADPH + H+
Caenorhabditis elegans
-
cholesta-5,7-dien-3beta-ol + NADP+ + H2O
-
-
?
1.14.19.21
cholesterol + O2 + NADPH + H+
Bombyx mori
-
cholesta-5,7-dien-3beta-ol + NADP+ + H2O
-
-
?
1.14.19.21
cholesterol + O2 + NADPH + H+
Xenopus laevis
-
cholesta-5,7-dien-3beta-ol + NADP+ + H2O
-
-
?
1.14.19.21
cholesterol + O2 + NADPH + H+
Danio rerio
-
cholesta-5,7-dien-3beta-ol + NADP+ + H2O
-
-
?
1.14.19.21
cholesterol + O2 + NADPH + H+
Ciona intestinalis
-
cholesta-5,7-dien-3beta-ol + NADP+ + H2O
-
-
?
1.14.19.21
cholesterol + O2 + NADPH + H+
Hemicentrotus pulcherrimus
-
cholesta-5,7-dien-3beta-ol + NADP+ + H2O
-
-
?
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.3.1.21
cholesterol + O2 + NAD(P)H + H+
-
725431
Caenorhabditis elegans
cholesta-5,7-dien-3beta-ol + NAD(P)+ + 2 H2O
-
-
-
?
1.3.1.21
cholesterol + O2 + NAD(P)H + H+
-
725431
Bombyx mori
cholesta-5,7-dien-3beta-ol + NAD(P)+ + 2 H2O
-
-
-
?
1.14.19.21
cholesterol + O2 + NADH + H+
-
725431
Bombyx mori
cholesta-5,7-dien-3beta-ol + NAD+ + H2O
-
-
-
?
1.14.19.21
cholesterol + O2 + NADH + H+
-
725431
Caenorhabditis elegans
cholesta-5,7-dien-3beta-ol + NADP+ + H2O
-
-
-
?
1.14.19.21
cholesterol + O2 + NADH + H+
-
725431
Xenopus laevis
cholesta-5,7-dien-3beta-ol + NADP+ + H2O
-
-
-
?
1.14.19.21
cholesterol + O2 + NADH + H+
-
725431
Danio rerio
cholesta-5,7-dien-3beta-ol + NADP+ + H2O
-
-
-
?
1.14.19.21
cholesterol + O2 + NADH + H+
-
725431
Ciona intestinalis
cholesta-5,7-dien-3beta-ol + NADP+ + H2O
-
-
-
?
1.14.19.21
cholesterol + O2 + NADH + H+
-
725431
Hemicentrotus pulcherrimus
cholesta-5,7-dien-3beta-ol + NADP+ + H2O
-
-
-
?
1.14.19.21
cholesterol + O2 + NADPH + H+
-
725431
Caenorhabditis elegans
cholesta-5,7-dien-3beta-ol + NADP+ + H2O
-
-
-
?
1.14.19.21
cholesterol + O2 + NADPH + H+
-
725431
Bombyx mori
cholesta-5,7-dien-3beta-ol + NADP+ + H2O
-
-
-
?
1.14.19.21
cholesterol + O2 + NADPH + H+
-
725431
Xenopus laevis
cholesta-5,7-dien-3beta-ol + NADP+ + H2O
-
-
-
?
1.14.19.21
cholesterol + O2 + NADPH + H+
-
725431
Danio rerio
cholesta-5,7-dien-3beta-ol + NADP+ + H2O
-
-
-
?
1.14.19.21
cholesterol + O2 + NADPH + H+
-
725431
Ciona intestinalis
cholesta-5,7-dien-3beta-ol + NADP+ + H2O
-
-
-
?
1.14.19.21
cholesterol + O2 + NADPH + H+
-
725431
Hemicentrotus pulcherrimus
cholesta-5,7-dien-3beta-ol + NADP+ + H2O
-
-
-
?
General Information
EC Number
General Information
Commentary
Organism
1.3.1.21
physiological function
wild-type Bombyx mori enzyme rescues the lethality of Drosophila melanogaster animals treated with RNAi against the nvd gene, whereas Bombyx mori mutants H190A and H282A do not
Bombyx mori
General Information (protein specific)
EC Number
General Information
Commentary
Organism
1.3.1.21
physiological function
wild-type Bombyx mori enzyme rescues the lethality of Drosophila melanogaster animals treated with RNAi against the nvd gene, whereas Bombyx mori mutants H190A and H282A do not
Bombyx mori