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Literature summary extracted from

  • Ong, H.B.; Sienkiewicz, N.; Wyllie, S.; Fairlamb, A.H.
    Dissecting the metabolic roles of pteridine reductase 1 in Trypanosoma brucei and Leishmania major (2011), J. Biol. Chem., 286, 10429-10438.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.33 dihydrobiopterin inhibits the enzyme at high substrate concentrations Leishmania major
1.5.1.33 dihydrobiopterin inhibits the enzyme at high substrate concentrations Trypanosoma brucei
1.5.1.33 quinonoid 6,7-dimethyl-7,8-dihydropterin
-
 Leishmania major
1.5.1.33 quinonoid 6,7-dimethyl-7,8-dihydropterin
-
 Trypanosoma brucei
1.5.1.33 quinonoid dihydrobiopterin
-
 Leishmania major
1.5.1.33 quinonoid dihydrobiopterin
-
 Trypanosoma brucei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.33 0.0000294
-
 dihydrobiopterin in 50 mM HEPES buffer, pH 7.4, at 25°C Trypanosoma brucei
1.5.1.33 0.0032
-
 dihydrobiopterin in 50 mM HEPES buffer, pH 7.4, at 25°C Leishmania major
1.5.1.33 0.0036
-
 quinonoid dihydrobiopterin in 50 mM HEPES buffer, pH 7.4, at 25°C Trypanosoma brucei
1.5.1.33 0.0103
-
 quinonoid dihydrobiopterin in 50 mM HEPES buffer, pH 7.4, at 25°C Leishmania major
1.5.1.33 0.0166
-
 quinonoid 6,7-dimethyl-7,8-dihydropterin in 50 mM HEPES buffer, pH 7.4, at 25°C Trypanosoma brucei
1.5.1.33 0.021
-
 quinonoid 6,7-dimethyl-7,8-dihydropterin in 50 mM HEPES buffer, pH 7.4, at 25°C Leishmania major

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.5.1.33 cytosol
-
 Trypanosoma brucei 5829
-
1.5.1.33 cytosol
-
 Leishmania major 5829
-

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.33 Leishmania major
-
-
-
1.5.1.33 Trypanosoma brucei
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.33 biopterin + NADPH + H+
-
 Trypanosoma brucei dihydrobiopterin + NADP+
-
 ?
1.5.1.33 biopterin + NADPH + H+
-
 Leishmania major dihydrobiopterin + NADP+
-
 ?
1.5.1.33 dihydrobiopterin + NADPH + H+
-
 Trypanosoma brucei 5,6,7,8-tetrahydrobiopterin + NADP+
-
 ?
1.5.1.33 dihydrobiopterin + NADPH + H+
-
 Leishmania major 5,6,7,8-tetrahydrobiopterin + NADP+
-
 ?
1.5.1.33 quinonoid 6,7-dimethyl-7,8-dihydropterin + NADPH + H+
-
 Trypanosoma brucei ?
-
 ?
1.5.1.33 quinonoid 6,7-dimethyl-7,8-dihydropterin + NADPH + H+
-
 Leishmania major ?
-
 ?
1.5.1.33 quinonoid dihydrobiopterin + NADPH + H+
-
 Trypanosoma brucei tetrahydrobiopterin + NADP+
-
 ?
1.5.1.33 quinonoid dihydrobiopterin + NADPH + H+
-
 Leishmania major tetrahydrobiopterin + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.5.1.33 pteridine reductase 1
-
 Trypanosoma brucei
1.5.1.33 pteridine reductase 1
-
 Leishmania major
1.5.1.33 PTR1
-
 Trypanosoma brucei
1.5.1.33 PTR1
-
 Leishmania major

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5.1.33 0.05
-
 dihydrobiopterin in 50 mM HEPES buffer, pH 7.4, at 25°C Trypanosoma brucei
1.5.1.33 0.43
-
 quinonoid 6,7-dimethyl-7,8-dihydropterin in 50 mM HEPES buffer, pH 7.4, at 25°C Trypanosoma brucei
1.5.1.33 0.44
-
 quinonoid 6,7-dimethyl-7,8-dihydropterin in 50 mM HEPES buffer, pH 7.4, at 25°C Leishmania major
1.5.1.33 0.54
-
 quinonoid dihydrobiopterin in 50 mM HEPES buffer, pH 7.4, at 25°C Trypanosoma brucei
1.5.1.33 0.57
-
 dihydrobiopterin in 50 mM HEPES buffer, pH 7.4, at 25°C Leishmania major
1.5.1.33 1.84
-
 quinonoid dihydrobiopterin in 50 mM HEPES buffer, pH 7.4, at 25°C Leishmania major

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.1.33 5.5 8
-
 Trypanosoma brucei
1.5.1.33 5.5 8
-
 Leishmania major

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.5.1.33 4 9
-
 Trypanosoma brucei
1.5.1.33 4 9
-
 Leishmania major

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.33 NADPH
-
 Trypanosoma brucei
1.5.1.33 NADPH
-
 Leishmania major

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.5.1.33 0.00015
-
 dihydrobiopterin in 50 mM HEPES buffer, pH 7.4, at 25°C Leishmania major
1.5.1.33 0.00116
-
 dihydrobiopterin in 50 mM HEPES buffer, pH 7.4, at 25°C Trypanosoma brucei
1.5.1.33 0.00122
-
 quinonoid dihydrobiopterin in 50 mM HEPES buffer, pH 7.4, at 25°C Leishmania major
1.5.1.33 0.00314
-
 quinonoid dihydrobiopterin in 50 mM HEPES buffer, pH 7.4, at 25°C Trypanosoma brucei
1.5.1.33 0.0922
-
 quinonoid 6,7-dimethyl-7,8-dihydropterin in 50 mM HEPES buffer, pH 7.4, at 25°C Leishmania major
1.5.1.33 0.475
-
 quinonoid 6,7-dimethyl-7,8-dihydropterin in 50 mM HEPES buffer, pH 7.4, at 25°C Trypanosoma brucei

General Information

EC Number General Information Comment Organism
1.5.1.33 physiological function the enzyme has an essential and dual role in pterin metabolism Trypanosoma brucei
1.5.1.33 physiological function the enzyme has an essential and dual role in pterin metabolism Leishmania major

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.5.1.33 21
-
 quinonoid 6,7-dimethyl-7,8-dihydropterin in 50 mM HEPES buffer, pH 7.4, at 25°C Leishmania major
1.5.1.33 26
-
 quinonoid 6,7-dimethyl-7,8-dihydropterin in 50 mM HEPES buffer, pH 7.4, at 25°C Trypanosoma brucei
1.5.1.33 160
-
 quinonoid dihydrobiopterin in 50 mM HEPES buffer, pH 7.4, at 25°C Trypanosoma brucei
1.5.1.33 170
-
 dihydrobiopterin in 50 mM HEPES buffer, pH 7.4, at 25°C Leishmania major
1.5.1.33 180
-
 quinonoid dihydrobiopterin in 50 mM HEPES buffer, pH 7.4, at 25°C Leishmania major
1.5.1.33 1700
-
 dihydrobiopterin in 50 mM HEPES buffer, pH 7.4, at 25°C Trypanosoma brucei