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Literature summary extracted from

  • French, J.B.; Ealick, S.E.
    Structural and mechanistic studies on Klebsiella pneumoniae 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase (2010), J. Biol. Chem., 285, 35446-35454.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.97
-
 Klebsiella pneumoniae

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.1.1.97 hanging-drop vapor-diffusion method at 18°C Klebsiella pneumoniae

Protein Variants

EC Number Protein Variants Comment Organism
4.1.1.97 Q88E the maximal rate of this mutant is 43fold slower than that of the native rate, whereas the measured Km for the mutant is similar to that of the native enzyme, the kcat/Km value is about 50fold lower than the wild-type value Klebsiella pneumoniae

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.97 allopurinol competitive inhibitor. The inhibitor disrupts the necessary organization of the active site Klebsiella pneumoniae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.97 0.151
-
 5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate mutant enzyme Q88E, pH and temperature not specified in the publication Klebsiella pneumoniae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.1.97 5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate Klebsiella pneumoniae the enzyme is part of the pathway from urate to (S)-allantoin (S)-allantoin + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.97 Klebsiella pneumoniae A6T925
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.97
-
 Klebsiella pneumoniae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.97 5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate
-
 Klebsiella pneumoniae (S)-allantoin + CO2
-
 ?
4.1.1.97 5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate the enzyme is part of the pathway from urate to (S)-allantoin Klebsiella pneumoniae (S)-allantoin + CO2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.1.1.97 hpxQ
-
 Klebsiella pneumoniae
4.1.1.97 OHCU decarboxylase
-
 Klebsiella pneumoniae
4.1.1.97 PRHOXNB
-
 Klebsiella pneumoniae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.1.97 122
-
 5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate mutant enzyme Q88E, pH and temperature not specified in the publication Klebsiella pneumoniae

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.1.1.97 0.03
-
 allopurinol mutant enzyme Q88E, pH and temperature not specified in the publication Klebsiella pneumoniae

General Information

EC Number General Information Comment Organism
4.1.1.97 physiological function the enzyme is part of the pathway from urate to (S)-allantoin Klebsiella pneumoniae

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.1.1.97 19
-
 5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate mutant enzyme Q88E, pH and temperature not specified in the publication Klebsiella pneumoniae
4.1.1.97 970
-
 5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate wild-type enzyme, pH and temperature not specified in the publication Klebsiella pneumoniae