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Literature summary extracted from

  • Arragain, S.; Handelman, S.K.; Forouhar, F.; Wei, F.Y.; Tomizawa, K.; Hunt, J.F.; Douki, T.; Fontecave, M.; Mulliez, E.; Atta, M.
    Identification of eukaryotic and prokaryotic methylthiotransferase for biosynthesis of 2-methylthio-N6-threonylcarbamoyladenosine in tRNA (2010), J. Biol. Chem., 285, 28425-28433.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.8.4.5 expression in Escherichia coli Bacillus subtilis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.8.4.5 [4Fe-4S]-center the enzyme contains two [4Fe-4S] clusters as well as the transferred sulfur. The sulfur donor is believed to be one of the [4Fe-4S] clusters Bacillus subtilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.8.4.5 50000
-
 x * 50000, SDS-PAGE Bacillus subtilis
2.8.4.5 51658
-
 x * 51658, calculated from sequence Bacillus subtilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.4.5 N6-L-threonylcarbamoyladenine37 in tRNA + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine Bacillus subtilis
-
 2-methylthio-N6-L-threonylcarbamoyladenine37 in tRNA + S-adenosyl-L-homocysteine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine
-
 ?
2.8.4.5 N6-L-threonylcarbamoyladenine37 in tRNA + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine Bacillus subtilis 168
-
 2-methylthio-N6-L-threonylcarbamoyladenine37 in tRNA + S-adenosyl-L-homocysteine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.8.4.5 Bacillus subtilis P54462
-
-
2.8.4.5 Bacillus subtilis 168 P54462
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.4.5
-
 Bacillus subtilis

Reaction

EC Number Reaction Comment Organism Reaction ID
2.8.4.5 N6-L-threonylcarbamoyladenine37 in tRNA + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + reduced electron acceptor = 2-(methylsulfanyl)-N6-L-threonylcarbamoyladenine37 in tRNA + S-adenosyl-L-homocysteine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine + electron acceptor (overall reaction) Bacillus subtilis
a
2.8.4.5 N6-L-threonylcarbamoyladenine37 in tRNA + sulfur-(sulfur carrier) + S-adenosyl-L-methionine + reduced electron acceptor = 2-sulfanyl-N6-L-threonylcarbamoyladenine37 in tRNA + (sulfur carrier) + L-methionine + 5'-deoxyadenosine + electron acceptor (1a) Bacillus subtilis
a
2.8.4.5 S-adenosyl-L-methionine + 2-sulfanyl-N6-L-threonylcarbamoyladenine37 in tRNA = S-adenosyl-L-homocysteine + 2-(methylsulfanyl)-N6-L-threonylcarbamoyladenine37 in tRNA (1b) Bacillus subtilis
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.4.5 N6-L-threonylcarbamoyladenine37 in tRNA + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine
-
 Bacillus subtilis 2-methylthio-N6-L-threonylcarbamoyladenine37 in tRNA + S-adenosyl-L-homocysteine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine
-
 ?
2.8.4.5 N6-L-threonylcarbamoyladenine37 in tRNA + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine
-
 Bacillus subtilis 168 2-methylthio-N6-L-threonylcarbamoyladenine37 in tRNA + S-adenosyl-L-homocysteine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.8.4.5 ? x * 50000, SDS-PAGE Bacillus subtilis
2.8.4.5 ? x * 51658, calculated from sequence Bacillus subtilis

Synonyms

EC Number Synonyms Comment Organism
2.8.4.5 CDKAL1
-
 Bacillus subtilis
2.8.4.5 methylthio-threonylcarbamoyl-adenosine transferase B
-
 Bacillus subtilis
2.8.4.5 MtaB
-
 Bacillus subtilis
2.8.4.5 YqeV
-
 Bacillus subtilis

Cofactor

EC Number Cofactor Comment Organism Structure
2.8.4.5 S-adenosyl-L-methionine the enzyme, which is a member of the S-adenosyl-L-methionine-dependent radical (radical AdoMet) enzymes superfamily Bacillus subtilis