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Literature summary extracted from

  • Kaysser, L.; Eitel, K.; Tanino, T.; Siebenberg, S.; Matsuda, A.; Ichikawa, S.; Gust, B.
    A new arylsulfate sulfotransferase involved in liponucleoside antibiotic biosynthesis in Streptomycetes (2010), J. Biol. Chem., 285, 12684-12694.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.8.2.22 EDTA the addition of 0.1 mM EDTA increases product formation by 2.5fold Streptomyces sp.

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.8.2.22 expressed in Escherichia coli Rosetta2 (DE3)pLys cells Streptomyces sp.

Protein Variants

EC Number Protein Variants Comment Organism
2.8.2.22 H180L the mutant shows less than 5% activity compared to the wild type enzyme Streptomyces sp.
2.8.2.22 H253L the mutant shows less than 5% activity compared to the wild type enzyme Streptomyces sp.
2.8.2.22 H308L the mutant shows less than 5% activity compared to the wild type enzyme Streptomyces sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.8.2.22 phenol
-
 Streptomyces sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.2.22 0.0259
-
 phenol using 4-nitrophenyl sulfate as cosubstrate, at pH 6.7, temperature not specified in the publication Streptomyces sp.
2.8.2.22 0.0297
-
 phenol using methylumbelliferone sulfate as cosubstrate, at pH 6.7, temperature not specified in the publication Streptomyces sp.
2.8.2.22 0.0345
-
 methylumbelliferone sulfate at pH 6.7, temperature not specified in the publication Streptomyces sp.
2.8.2.22 0.0481
-
 4-nitrophenyl sulfate at pH 6.7, temperature not specified in the publication Streptomyces sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.8.2.22 additional information the presence of 20 mM Mg2+ or 2 mM Ni2ü in the assay leads to the precipitation of the protein Streptomyces sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.8.2.22 58000
-
 x * 58000, SDS-PAGE Streptomyces sp.

Organism

EC Number Organism UniProt Comment Textmining
2.8.2.22 Streptomyces sp. D6N7U5
-
-
2.8.2.22 Streptomyces sp. MK730-62F2 D6N7U5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.2.22 nickel affinity column chromatography, and Superdex 200 gel filtration Streptomyces sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.2.22 4-nitrophenyl sulfate + (5S)-5'-O-(5-amino-5-deoxy-beta-D-ribofuranosyl)-5'-C-[(2S,5S,6S)-5-carboxy-6-(hexadecanoyloxy)-4-methyl-3-oxo-1,4-diazepan-2-yl]uridine highly efficient substrate Streptomyces sp. ?
-
 ?
2.8.2.22 4-nitrophenyl sulfate + (5S)-5'-O-(5-amino-5-deoxy-beta-D-ribofuranosyl)-5'-C-[(2S,5S,6S)-5-carboxy-6-(hexadecanoyloxy)-4-methyl-3-oxo-1,4-diazepan-2-yl]uridine highly efficient substrate Streptomyces sp. MK730-62F2 ?
-
 ?
2.8.2.22 4-nitrophenyl sulfate + phenol ping pong bi-bi mechanism Streptomyces sp. 4-nitrophenol + phenyl sulfate
-
 ?
2.8.2.22 4-nitrophenyl sulfate + phenol ping pong bi-bi mechanism Streptomyces sp. MK730-62F2 4-nitrophenol + phenyl sulfate
-
 ?
2.8.2.22 methylumbelliferone sulfate + phenol ping pong bi-bi mechanism Streptomyces sp. methylumbelliferone + phenyl sulfate
-
 ?
2.8.2.22 methylumbelliferone sulfate + phenol ping pong bi-bi mechanism Streptomyces sp. MK730-62F2 methylumbelliferone + phenyl sulfate
-
 ?
2.8.2.22 additional information no product formation observed with 3'-phosphoadenosine 5'-phosphosulfate as the donor substrate Streptomyces sp. ?
-
 ?
2.8.2.22 additional information no product formation observed with 3'-phosphoadenosine 5'-phosphosulfate as the donor substrate Streptomyces sp. MK730-62F2 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.8.2.22 ? x * 58000, SDS-PAGE Streptomyces sp.

Synonyms

EC Number Synonyms Comment Organism
2.8.2.22 arylsulfate sulfotransferase
-
 Streptomyces sp.
2.8.2.22 Cpz4
-
 Streptomyces sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.8.2.22 0.14
-
 4-nitrophenyl sulfate at pH 6.7, temperature not specified in the publication Streptomyces sp.
2.8.2.22 0.15
-
 methylumbelliferone sulfate at pH 6.7, temperature not specified in the publication Streptomyces sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.8.2.22 6.7
-
-
 Streptomyces sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.8.2.22 5 7.6 maximal activity is observed at pH 6.7 with half-maximal values at pH 5.0 and 7.6 Streptomyces sp.

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.8.2.22 41.7
-
 phenol using 4-nitrophenol sulfate as substrate, at pH 6.7, temperature not specified in the publication Streptomyces sp.
2.8.2.22 46.1
-
 phenol using methyl umbelliferone sulfate as substrate at pH 6.7, temperature not specified in the publication Streptomyces sp.