Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Saleh, O.; Gust, B.; Boll, B.; Fiedler, H.P.; Heide, L.
    Aromatic prenylation in phenazine biosynthesis: dihydrophenazine-1-carboxylate dimethylallyltransferase from Streptomyces anulatus (2009), J. Biol. Chem., 284, 14439-14447.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.5.1.121 EDTA 10 mM, increases reaction velocity 1.5fold Streptomyces anulatus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.121 heterologous expression in Streptomyces coelicolor M512 Streptomyces anulatus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.121 FeSO4 10 mM, completely abolishes the formation of endophenazine A Streptomyces anulatus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.121 0.035
-
 5,10-dihydrophenazine 1-carboxylate pH 7.5, 30°C Streptomyces anulatus
2.5.1.121 0.116
-
 dimethylallyl diphosphate pH 7.5, 30°C Streptomyces anulatus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.121 Ca2+ 100 mM MgCl2 increases product formation 1.5fold Streptomyces anulatus
2.5.1.121 Mg2+ the enzyme is independent of the presence of magnesium or other divalent cations. 50 mM MgCl2 increases product formation 1.5fold Streptomyces anulatus
2.5.1.121 Na+ 500 mM, NaCl increases product formation 3fold Streptomyces anulatus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.121 37000
-
 x * 37000, SDS-PAGE Streptomyces anulatus
2.5.1.121 37138
-
 x * 37138, calculated from sequence Streptomyces anulatus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.121 dimethylallyl diphosphate + 5,10-dihydrophenazine 1-carboxylate Streptomyces anulatus the enzyme is involved in the biosynthesis of prenylated phenazines diphosphate + 5,10-dihydro-9-dimethylallylphenazine 1-carboxylate
-
 ?
2.5.1.121 dimethylallyl diphosphate + 5,10-dihydrophenazine 1-carboxylate Streptomyces anulatus 9663 the enzyme is involved in the biosynthesis of prenylated phenazines diphosphate + 5,10-dihydro-9-dimethylallylphenazine 1-carboxylate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.121 Streptomyces anulatus C4PWA1
-
-
2.5.1.121 Streptomyces anulatus 9663 C4PWA1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.121
-
 Streptomyces anulatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.121 dimethylallyl diphosphate + 5,10-dihydrophenazine 1-carboxylate the enzyme is involved in the biosynthesis of prenylated phenazines Streptomyces anulatus diphosphate + 5,10-dihydro-9-dimethylallylphenazine 1-carboxylate
-
 ?
2.5.1.121 dimethylallyl diphosphate + 5,10-dihydrophenazine 1-carboxylate the enzyme is specific for both dimethylallyl diphosphate and 5,10-dihydrophenazine 1-carboxylate. No activity with geranyldiphosphate or other phenazine substrates, such as phenazine, phenazine 1-carboxylic acid methyl ester, N-methyl-phenazine, or pyocyanine (1-hydroxy-5-N-methyl-phenazine). No activity with 4-hydroxyphenylpyruvate 1,3-dihydroxynaphthalene, and 1,6-dihydroxynaphthalene. Reaction velocity with flaviolin is 0.5% compared to the activity with 5,10-dihydrophenazine 1-carboxylate Streptomyces anulatus diphosphate + 5,10-dihydro-9-dimethylallylphenazine 1-carboxylate
-
 ?
2.5.1.121 dimethylallyl diphosphate + 5,10-dihydrophenazine 1-carboxylate the enzyme is involved in the biosynthesis of prenylated phenazines Streptomyces anulatus 9663 diphosphate + 5,10-dihydro-9-dimethylallylphenazine 1-carboxylate
-
 ?
2.5.1.121 dimethylallyl diphosphate + 5,10-dihydrophenazine 1-carboxylate the enzyme is specific for both dimethylallyl diphosphate and 5,10-dihydrophenazine 1-carboxylate. No activity with geranyldiphosphate or other phenazine substrates, such as phenazine, phenazine 1-carboxylic acid methyl ester, N-methyl-phenazine, or pyocyanine (1-hydroxy-5-N-methyl-phenazine). No activity with 4-hydroxyphenylpyruvate 1,3-dihydroxynaphthalene, and 1,6-dihydroxynaphthalene. Reaction velocity with flaviolin is 0.5% compared to the activity with 5,10-dihydrophenazine 1-carboxylate Streptomyces anulatus 9663 diphosphate + 5,10-dihydro-9-dimethylallylphenazine 1-carboxylate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.121 ? x * 37000, SDS-PAGE Streptomyces anulatus
2.5.1.121 ? x * 37138, calculated from sequence Streptomyces anulatus

Synonyms

EC Number Synonyms Comment Organism
2.5.1.121 5,10-dihydrophenazine 1-carboxylate dimethylallyltransferase
-
 Streptomyces anulatus
2.5.1.121 dihydrophenazine-1-carboxylate dimethylallyltransferase
-
 Streptomyces anulatus
2.5.1.121 Ppzp
-
 Streptomyces anulatus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.121 0.435
-
 5,10-dihydrophenazine 1-carboxylate pH 7.5, 30°C Streptomyces anulatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.121 7.5
-
-
 Streptomyces anulatus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.5.1.121 5 9.5 pH 5.0: about 50% of maximal activity, ph 9.5: about 50% of maximal activity Streptomyces anulatus

General Information

EC Number General Information Comment Organism
2.5.1.121 malfunction after inactivation of ppzP, only nonprenylated phenazine 1-carboxylic acid is formed Streptomyces anulatus
2.5.1.121 physiological function the enzyme is involved in the biosynthesis of prenylated phenazines Streptomyces anulatus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.5.1.121 12.4
-
 5,10-dihydrophenazine 1-carboxylate pH 7.5, 30°C Streptomyces anulatus