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Literature summary extracted from

  • Pucci, B.; De Felice, M.; Rossi, M.; Onesti, S.; Pisani, F.M.
    Amino acids of the Sulfolobus solfataricus mini-chromosome maintenance-like DNA helicase involved in DNA binding/remodeling (2004), J. Biol. Chem., 279, 49222-49228.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
5.6.2.4 H146A mutant enzyme is unable to bind DNA either in single- or double-stranded form and does not display helicase activity Saccharolobus solfataricus
5.6.2.4 H146A the purified mutant enzyme retains full ATPase activity Saccharolobus solfataricus
5.6.2.4 K129A mutant enzyme is unable to bind DNA either in single- or double-stranded form and does not display helicase activity Saccharolobus solfataricus
5.6.2.4 K129A the purified mutant enzyme retains full ATPase activity Saccharolobus solfataricus
5.6.2.4 K134A mutation affects binding to duplex DNA molecules, whereas it has no effect on binding to single-stranded DNA and on the DNA unwinding activity Saccharolobus solfataricus
5.6.2.4 K134A the purified mutant enzyme retains full ATPase activity Saccharolobus solfataricus
5.6.2.4 K194A mutant enzyme is unable to bind DNA either in single- or double-stranded form and does not display helicase activity Saccharolobus solfataricus
5.6.2.4 K194A the purified mutant enzyme retains full ATPase activity Saccharolobus solfataricus

Organism

EC Number Organism UniProt Comment Textmining
5.6.2.4 Methanothermobacter thermautotrophicus
-
-
-
5.6.2.4 Saccharolobus solfataricus Q9UXG1
-
-
5.6.2.4 Saccharolobus solfataricus P2 Q9UXG1
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.6.2.4 ATP + H2O DNA duplexes that contains a 30-nucleotide 5'-tail. The ability of the enzyme to bind single- but not double-stranded DNA is required for the unwinding function Saccharolobus solfataricus ADP + phosphate
-
 ?
5.6.2.4 ATP + H2O DNA duplexes that contains a 30-nucleotide 5'-tail. The ability of the enzyme to bind single- but not double-stranded DNA is required for the unwinding function Saccharolobus solfataricus P2 ADP + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
5.6.2.4 MCM-like DNA helicase
-
 Methanothermobacter thermautotrophicus
5.6.2.4 MCM-like DNA helicase
-
 Saccharolobus solfataricus
5.6.2.4 mini-chromosome maintenance-like DNA helicase
-
 Methanothermobacter thermautotrophicus
5.6.2.4 mini-chromosome maintenance-like DNA helicase
-
 Saccharolobus solfataricus
5.6.2.4 SsoMCM
-
 Methanothermobacter thermautotrophicus
5.6.2.4 SsoMCM
-
 Saccharolobus solfataricus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.6.2.4 70
-
 assay at Saccharolobus solfataricus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.6.2.4 7.5
-
 assay at Saccharolobus solfataricus