Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kiyonari, S.; Egashira, Y.; Ishino, S.; Ishino, Y.
    Biochemical characterization of endonuclease V from the hyperthermophilic archaeon, Pyrococcus furiosus (2014), J. Biochem., 155, 325-333.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.21.7 expression in Escherichia coli Pyrococcus furiosus

Protein Variants

EC Number Protein Variants Comment Organism
3.1.21.7 D35A inactive mutant enzyme Pyrococcus furiosus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.21.7 NaCl 200 mM, complete inhibition Pyrococcus furiosus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.21.7 Mg2+ activity is stimulated by the presence of Mg2+ or Mn2+ in the reaction buffer, with an optimum at 1 mM Mg2+ Pyrococcus furiosus
3.1.21.7 Mn2+ activity is stimulated by the presence of Mg2+ or Mn2+ in the reaction buffer, with an optimum at 1 mM Mg2+ Pyrococcus furiosus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.21.7 DNA + H2O Pyrococcus furiosus endonuclease V recognizes deoxyinosine and cleaves the second phosphodiester bond on the 3' side of the deaminated base lesion in damaged DNA. The enzyme possesses specific endonuclease activity for the deoxyinosine-containing DNA strand ?
-
 ?
3.1.21.7 nicked DNA + H2O Pyrococcus furiosus
-
 ?
-
 ?
3.1.21.7 RNA + H2O Pyrococcus furiosus the enzyme also cleaves inosine-containing RNA strands ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.21.7 Pyrococcus furiosus Q8U263
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.21.7
-
 Pyrococcus furiosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.21.7 DNA + H2O endonuclease V recognizes deoxyinosine and cleaves the second phosphodiester bond on the 3' side of the deaminated base lesion in damaged DNA. The enzyme possesses specific endonuclease activity for the deoxyinosine-containing DNA strand Pyrococcus furiosus ?
-
 ?
3.1.21.7 dsDNA containing deoxyinosine + H2O no cleavage activity is detected with the dsDNA containing a T/G mismatch base pair and a dsDNA containing an apurinic site. The enzyme possesses strict substrate specificity to deoxyinosine-containing DNA. The enzyme cleaves inosine-containing RNA strands as well as DNA substrates Pyrococcus furiosus ?
-
 ?
3.1.21.7 nicked DNA + H2O
-
 Pyrococcus furiosus ?
-
 ?
3.1.21.7 RNA + H2O the enzyme also cleaves inosine-containing RNA strands Pyrococcus furiosus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.21.7 endo V
-
 Pyrococcus furiosus
3.1.21.7 endonuclease V
-
 Pyrococcus furiosus
3.1.21.7 EndoV
-
 Pyrococcus furiosus
3.1.21.7 PF0987 locus name Pyrococcus furiosus
3.1.21.7 PfuEndoV
-
 Pyrococcus furiosus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.21.7 60
-
 a moderate reaction temperature is used for the biochemical studies, because of the melting temperature of the DNA duplex substrates (Tm = 67ºC) Pyrococcus furiosus
3.1.21.7 90
-
-
 Pyrococcus furiosus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.21.7 60 100 60°C: about 45% of maximal activity, 100°C: about 70% of maximal activity Pyrococcus furiosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.21.7 8.5
-
-
 Pyrococcus furiosus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.21.7 7 8.8 pH 7.0: about 45% of maximal activity, pH 8.8: about 90% of maximal activity Pyrococcus furiosus

General Information

EC Number General Information Comment Organism
3.1.21.7 metabolism this DNA repair enzyme is also involved in RNA metabolism Pyrococcus furiosus
3.1.21.7 physiological function DNA repair enzyme, may also be involved in RNA metabolism Pyrococcus furiosus