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Literature summary extracted from

  • Matsuda, T.; Sakaguchi, K.; Kobayashi, T.; Abe, E.; Kurano, N.; Sato, A.; Okita, Y.; Sugimoto, S.; Hama, Y.; Hayashi, M.; Okino, N.; Ito, M.
    Molecular cloning of a Pinguiochrysis pyriformis oleate-specific microsomal DELTA12-fatty acid desaturase and functional analysis in yeasts and thraustochytrids (2011), J. Biochem., 150, 375-383.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.19.6 expressed in Saccharomyces cerevisiae INVSc1 cells and Aurantiochytrium limacinum mh0186 cells Pinguiochrysis pyriformis
1.14.19.22 flag-tagged enzyme is functionally expressed in yeast cells. To express the gene in thraustochytrids, a construct driven by the thraustochytrid-derived ubiquitin promoter is used Pinguiochrysis pyriformis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.19.6 microsome
-
 Pinguiochrysis pyriformis
-
-
1.14.19.22 microsome
-
 Pinguiochrysis pyriformis
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.19.6 51100
-
 x * 51100, FLAG-tagged enzyme, SDS-PAGE Pinguiochrysis pyriformis
1.14.19.22 51000
-
 SDS-PAGE Pinguiochrysis pyriformis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.19.6 oleic acid + reduced acceptor + O2 Pinguiochrysis pyriformis specific substrate linoleic acid + acceptor + H2O
-
 ?
1.14.19.6 oleic acid + reduced acceptor + O2 Pinguiochrysis pyriformis MBIC 10872 specific substrate linoleic acid + acceptor + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.19.6 Pinguiochrysis pyriformis
-
-
-
1.14.19.6 Pinguiochrysis pyriformis MBIC 10872
-
-
-
1.14.19.22 Pinguiochrysis pyriformis
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.19.6 oleic acid + reduced acceptor + O2 specific substrate Pinguiochrysis pyriformis linoleic acid + acceptor + H2O
-
 ?
1.14.19.6 oleic acid + reduced acceptor + O2 specific substrate Pinguiochrysis pyriformis MBIC 10872 linoleic acid + acceptor + H2O
-
 ?
1.14.19.22 additional information exogenously added oleic acid is converted to linoleic acid in the gene transformants but not mock transformants of Aurantiochytrium limacinum mh0186 and yeast cells Pinguiochrysis pyriformis ?
-
 ?
1.14.19.22 additional information exogenously added oleic acid is converted to linoleic acid in the gene transformants but not mock transformants of Aurantiochytrium limacinum mh0186 and yeast cells Pinguiochrysis pyriformis MBIC 10872 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.19.6 ? x * 51100, FLAG-tagged enzyme, SDS-PAGE Pinguiochrysis pyriformis

Synonyms

EC Number Synonyms Comment Organism
1.14.19.6 DELTA12-fatty acid desaturase
-
 Pinguiochrysis pyriformis
1.14.19.6 Des12
-
 Pinguiochrysis pyriformis
1.14.19.22 DELTA12-fatty acid desaturase
-
 Pinguiochrysis pyriformis