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Literature summary extracted from

  • D'Auria, S.; Nucci, R.; Rossi, M.; Bertoli, E.; Tanfani, F.; Gryczynski, I.; Malak, H.; Lakowicz, J.R.
    beta-Glycosidase from the hyperthermophilic archaeon Sulfolobus solfataricus: structure and activity in the presence of alcohols (1999), J. Biochem., 126, 545-552.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.B34 1-butanol maximal activation is induced by 80 mM 1-butanol (130%). The activation at 30"C becomes less with increasing temperature and at 75°C is only 10%. Alcohol modifies the protein microenvironment, leading to a more flexible enzyme structure, which is probably responsible for the enhanced enzymatic activity Sulfolobus acidocaldarius
3.2.1.B34 1-propanol alcohol modifies the protein microenvironment, leading to a more flexible enzyme structure, which is probably responsible for the enhanced enzymatic activity Sulfolobus acidocaldarius
3.2.1.B34 ethanol alcohol modifies the protein microenvironment, leading to a more flexible enzyme structure, which is probably responsible for the enhanced enzymatic activity Sulfolobus acidocaldarius
3.2.1.B34 methanol alcohol modifies the protein microenvironment, leading to a more flexible enzyme structure, which is probably responsible for the enhanced enzymatic activity Sulfolobus acidocaldarius

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.B34 expressed in Escherichia coli Sulfolobus acidocaldarius

General Stability

EC Number General Stability Organism
3.2.1.B34 stable in the presence of detergents Sulfolobus acidocaldarius

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.B34 0.5
-
 2-nitrophenyl beta-D-galactopyranoside pH 7.4, 30°C, presence of 80 mM 1-butanol Sulfolobus acidocaldarius
3.2.1.B34 1
-
 2-nitrophenyl beta-D-galactopyranoside pH 7.4, 30°C, absence of 1-butanol Sulfolobus acidocaldarius
3.2.1.B34 4
-
 2-nitrophenyl beta-D-galactopyranoside pH 7.4, 75°C, absence of 1-butanol Sulfolobus acidocaldarius
3.2.1.B34 11
-
 2-nitrophenyl beta-D-galactopyranoside pH 7.4, 75°C, presence of 80 mM 1-butanol Sulfolobus acidocaldarius

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.B34 240000
-
-
 Sulfolobus acidocaldarius

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.B34 Sulfolobus acidocaldarius
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.B34
-
 Sulfolobus acidocaldarius

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.B34 2-nitrophenyl beta-D-galactopyranoside + H2O
-
 Sulfolobus acidocaldarius 2-nitrophenol + beta-D-galactopyranose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.B34 tetramer
-
 Sulfolobus acidocaldarius

Synonyms

EC Number Synonyms Comment Organism
3.2.1.B34 Sbetagly
-
 Sulfolobus acidocaldarius

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.B34 95
-
-
 Sulfolobus acidocaldarius

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.B34 24
-
 2-nitrophenyl beta-D-galactopyranoside pH 7.4, 30°C, absence of 1-butanol Sulfolobus acidocaldarius
3.2.1.B34 55
-
 2-nitrophenyl beta-D-galactopyranoside pH 7.4, 30°C, presence of 80 mM 1-butanol Sulfolobus acidocaldarius
3.2.1.B34 1320
-
 2-nitrophenyl beta-D-galactopyranoside pH 7.4, 75°C, absence of 1-butanol Sulfolobus acidocaldarius
3.2.1.B34 1450
-
 2-nitrophenyl beta-D-galactopyranoside pH 7.4, 75°C, presence of 80 mM 1-butanol Sulfolobus acidocaldarius

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.B34 7.4
-
 assay at Sulfolobus acidocaldarius

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.B34 24
-
 2-nitrophenyl beta-D-galactopyranoside pH 7.4, 30°C, absence of 1-butanol Sulfolobus acidocaldarius
3.2.1.B34 110
-
 2-nitrophenyl beta-D-galactopyranoside pH 7.4, 30°C, presence of 80 mM 1-butanol Sulfolobus acidocaldarius
3.2.1.B34 131.9
-
 2-nitrophenyl beta-D-galactopyranoside pH 7.4, 75°C, presence of 80 mM 1-butanol Sulfolobus acidocaldarius
3.2.1.B34 330
-
 2-nitrophenyl beta-D-galactopyranoside pH 7.4, 75°C, absence of 1-butanol Sulfolobus acidocaldarius