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Literature summary extracted from

  • Kim, H.G.; Han, G.H.; Kim, D.; Choi, J.S.; Kim, S.W.
    Comparative analysis of two types of methanol dehydrogenase from Methylophaga aminisulfidivorans MPT grown on methanol (2012), J. Basic Microbiol., 52, 141-149.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.2.7 mxaFJGIR gene cluster encoding the large and small subunits, and protein mxaJ, organized in an operon and are transcribed as a single mRNA, DNA and amino acid sequence determination and analysis Methylophaga aminisulfidivorans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.2.7 0.013
-
 methanol type II MDH, pH 7.0, 30°C, with cytochrome cL Methylophaga aminisulfidivorans
1.1.2.7 0.0503
-
 methanol type I MDH, pH 7.0, 30°C, with cytochrome cL Methylophaga aminisulfidivorans
1.1.2.7 0.0511
-
 methanol type II MDH, pH 9.0, 30°C, with phenazine ethosulfate and 2,6-dichlorophenolindophenol Methylophaga aminisulfidivorans
1.1.2.7 0.1046
-
 methanol type I MDH, pH 9.0, 30°C, with phenazine ethosulfate and 2,6-dichlorophenolindophenol Methylophaga aminisulfidivorans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.2.7 periplasm
-
 Methylophaga aminisulfidivorans
-
-
1.1.2.7 soluble
-
 Methylophaga aminisulfidivorans
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.2.7 7580
-
 2 * 65980, subunit alpha, + 2 * 7580, subunit beta, + 1 * 27860, MxaJ protein, type II MDH consists of two identical dimers of alpha and beta subunits organized to form the alpha2beta2 tetramer, and contains an additional MxaJ protein Methylophaga aminisulfidivorans
1.1.2.7 7580
-
 2 * 65980, subunit alpha, + 2 * 7580, subunit beta, type I MDH consists of two identical dimers of alpha and beta subunits organized to form the alpha2beta2 tetramer Methylophaga aminisulfidivorans
1.1.2.7 27860
-
 2 * 65980, subunit alpha, + 2 * 7580, subunit beta, + 1 * 27860, MxaJ protein, type II MDH consists of two identical dimers of alpha and beta subunits organized to form the alpha2beta2 tetramer, and contains an additional MxaJ protein Methylophaga aminisulfidivorans
1.1.2.7 65980
-
 2 * 65980, subunit alpha, + 2 * 7580, subunit beta, + 1 * 27860, MxaJ protein, type II MDH consists of two identical dimers of alpha and beta subunits organized to form the alpha2beta2 tetramer, and contains an additional MxaJ protein Methylophaga aminisulfidivorans
1.1.2.7 65980
-
 2 * 65980, subunit alpha, + 2 * 7580, subunit beta, type I MDH consists of two identical dimers of alpha and beta subunits organized to form the alpha2beta2 tetramer Methylophaga aminisulfidivorans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.2.7 methanol + 2 cytochrome cL Methylophaga aminisulfidivorans
-
 formaldehyde + 2 reduced cytochrome cL
-
 ?
1.1.2.7 methanol + 2 cytochrome cL Methylophaga aminisulfidivorans MPT
-
 formaldehyde + 2 reduced cytochrome cL
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.2.7 Methylophaga aminisulfidivorans A3FJ49 gene mxaJ; mxaFJGIR gene cluster encoding the large and small subunits, and protein mxaJ, organized in an operon and are transcribed as a single mRNA
-
1.1.2.7 Methylophaga aminisulfidivorans A3FJ51 small subunit; mxaFJGIR gene cluster encoding the large and small subunits, and protein mxaJ, organized in an operon and are transcribed as a single mRNA
-
1.1.2.7 Methylophaga aminisulfidivorans MPT A3FJ49 gene mxaJ; mxaFJGIR gene cluster encoding the large and small subunits, and protein mxaJ, organized in an operon and are transcribed as a single mRNA
-
1.1.2.7 Methylophaga aminisulfidivorans MPT A3FJ51 small subunit; mxaFJGIR gene cluster encoding the large and small subunits, and protein mxaJ, organized in an operon and are transcribed as a single mRNA
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.2.7 native isozymes by anion exchange chromatography and gel filtration, type I 7.9fold, type II 14.7fold, the lysozyme and freeze-thawing cell disruption method significantly increases the amount of type II MDH in the soluble fraction compared with strong physical disruption methods such as sonication and French Press Methylophaga aminisulfidivorans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.2.7 0.69
-
 type I MDH, pH 7.0, 30°C, with cytochrome cL Methylophaga aminisulfidivorans
1.1.2.7 0.75
-
 type II MDH, pH 7.0, 30°C, with cytochrome cL Methylophaga aminisulfidivorans
1.1.2.7 18.66
-
 type I MDH, pH 9.0, 30°C, with phenazine ethosulfate and 2,6-dichlorophenolindophenol Methylophaga aminisulfidivorans
1.1.2.7 21.03
-
 type II MDH, pH 9.0, 30°C, with phenazine ethosulfate and 2,6-dichlorophenolindophenol Methylophaga aminisulfidivorans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.2.7 methanol + 2 2,6-dichlorophenolindophenol in the activity assay phenazine ethosulfate is used as primary electron acceptor, and 2,6-dichlorophenol indophenol as a terminal acceptor Methylophaga aminisulfidivorans formaldehyde + 2 reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.2.7 methanol + 2 2,6-dichlorophenolindophenol in the activity assay phenazine ethosulfate is used as primary electron acceptor, and 2,6-dichlorophenolindophenol as a terminal acceptor Methylophaga aminisulfidivorans formaldehyde + 2 reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.2.7 methanol + 2 2,6-dichlorophenolindophenol in the activity assay phenazine ethosulfate is used as primary electron acceptor, and 2,6-dichlorophenol indophenol as a terminal acceptor Methylophaga aminisulfidivorans MPT formaldehyde + 2 reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.2.7 methanol + 2 2,6-dichlorophenolindophenol in the activity assay phenazine ethosulfate is used as primary electron acceptor, and 2,6-dichlorophenolindophenol as a terminal acceptor Methylophaga aminisulfidivorans MPT formaldehyde + 2 reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.2.7 methanol + 2 cytochrome cL
-
 Methylophaga aminisulfidivorans formaldehyde + 2 reduced cytochrome cL
-
 ?
1.1.2.7 methanol + 2 cytochrome cL native enzyme with pyrroloquinoline quinone as a prosthetic group and cytochrome cL as the primary electron acceptor Methylophaga aminisulfidivorans formaldehyde + 2 reduced cytochrome cL
-
 ?
1.1.2.7 methanol + 2 cytochrome cL
-
 Methylophaga aminisulfidivorans MPT formaldehyde + 2 reduced cytochrome cL
-
 ?
1.1.2.7 methanol + 2 cytochrome cL native enzyme with pyrroloquinoline quinone as a prosthetic group and cytochrome cL as the primary electron acceptor Methylophaga aminisulfidivorans MPT formaldehyde + 2 reduced cytochrome cL
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.2.7 More the original conformation of the MDH Methylophaga aminisulfidivorans MPT is most likely the alpha2beta2-MxaJ complex Methylophaga aminisulfidivorans
1.1.2.7 pentamer 2 * 65980, subunit alpha, + 2 * 7580, subunit beta, + 1 * 27860, MxaJ protein, type II MDH consists of two identical dimers of alpha and beta subunits organized to form the alpha2beta2 tetramer, and contains an additional MxaJ protein Methylophaga aminisulfidivorans
1.1.2.7 tetramer 2 * 65980, subunit alpha, + 2 * 7580, subunit beta, type I MDH consists of two identical dimers of alpha and beta subunits organized to form the alpha2beta2 tetramer Methylophaga aminisulfidivorans

Synonyms

EC Number Synonyms Comment Organism
1.1.2.7 type I MDH
-
 Methylophaga aminisulfidivorans
1.1.2.7 type II MDH
-
 Methylophaga aminisulfidivorans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.2.7 30
-
-
 Methylophaga aminisulfidivorans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.2.7 0.11
-
 methanol type I MDH, pH 9.0, 30°C, with phenazine ethosulfate and 2,6-dichlorophenolindophenol Methylophaga aminisulfidivorans
1.1.2.7 0.16
-
 methanol type II MDH, pH 9.0, 30°C, with phenazine ethosulfate and 2,6-dichlorophenolindophenol Methylophaga aminisulfidivorans
1.1.2.7 2.96
-
 methanol type I MDH, pH 7.0, 30°C, with cytochrome cL Methylophaga aminisulfidivorans
1.1.2.7 4.51
-
 methanol type II MDH, pH 7.0, 30°C, with cytochrome cL Methylophaga aminisulfidivorans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.2.7 8
-
-
 Methylophaga aminisulfidivorans

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.2.7 pyrroloquinoline quinone
-
 Methylophaga aminisulfidivorans

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.1.2.7 Methylophaga aminisulfidivorans type I MDH
-
 5.4
1.1.2.7 Methylophaga aminisulfidivorans type II MDH
-
 5.8

General Information

EC Number General Information Comment Organism
1.1.2.7 additional information type II MDH has higher specific activity than type I MDH. The original conformation of the MDH Methylophaga aminisulfidivorans MPT is most likely the alpha2beta2-MxaJ complex. The lysozyme and freeze-thawing cell disruption method significantly increases the amount of type II MDH in the soluble fraction compared with strong physical disruption methods such as sonication and French Press Methylophaga aminisulfidivorans