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Literature summary extracted from

  • Awano, T.; Wilming, A.; Tomita, H.; Yokooji, Y.; Fukui, T.; Imanaka, T.; Atomi, H.
    Characterization of two members among the five ADP-forming acyl coenzyme A (acyl-CoA) synthetases reveals the presence of a 2-(imidazol-4-yl)acetyl-CoA synthetase in Thermococcus kodakarensis (2014), J. Bacteriol., 196, 140-147.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.2.1.B10 coexpression with gene TK0943 in Escherichia coli Thermococcus kodakarensis
6.2.1.B11 coexpression of TK0944 and TK0943 in Escherichia coli Thermococcus kodakarensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.2.1.B10 additional information
-
 2-(imidazol-4-yl)acetate pH 6.5, 55°C, kinetic constant according to Adair-Pauling kinetic model: 0.543 mM Thermococcus kodakarensis
6.2.1.B10 0.03
-
 ATP pH 6.5, 55°C Thermococcus kodakarensis
6.2.1.B10 0.0625
-
 CoA pH 6.5, 55°C Thermococcus kodakarensis
6.2.1.B11 additional information
-
 Isobutyrate pH 6.5, 55°C, kinetic constant according to Adair-Pauling kinetic model: 0.132 mMs Thermococcus kodakarensis
6.2.1.B11 additional information
-
 thioglycolate pH 6.5, 55°C, kinetic constant according to Adair-Pauling kinetic model: 0.322 mM Thermococcus kodakarensis
6.2.1.B11 additional information
-
 phenylacetate pH 6.5, 55°C, kinetic constant according to Adair-Pauling kinetic model: 0.329 mM Thermococcus kodakarensis
6.2.1.B11 additional information
-
 acetate pH 6.5, 55°C, kinetic constant according to Adair-Pauling kinetic model: 1.5 mM Thermococcus kodakarensis
6.2.1.B11 0.0056
-
 CoA pH 6.5, 55°C Thermococcus kodakarensis
6.2.1.B11 0.0556
-
 ATP pH 6.5, 55°C Thermococcus kodakarensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.2.1.B10 27148
-
 2 * 47645 + 2 * 27148, calculated from sequence Thermococcus kodakarensis
6.2.1.B10 47645
-
 2 * 47645 + 2 * 27148, calculated from sequence Thermococcus kodakarensis
6.2.1.B11 27148
-
 2 * 51861 (TK0944) + 2 * 27148 (TK0943), calculated from sequence Thermococcus kodakarensis
6.2.1.B11 51861
-
 2 * 51861 (TK0944) + 2 * 27148 (TK0943), calculated from sequence Thermococcus kodakarensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.2.1.B10 ATP + 2-(imidazol-4-yl)acetate + CoA Thermococcus kodakarensis the enzyme is involved in catabolism of histidine ADP + phosphate + 2-(imidazol-4-yl)acetyl-CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.B10 Thermococcus kodakarensis Q5JEW2 and Q5JIA9 Q5JEW2: alpha-subunit, Q5JIA9: beta-subunit
-
6.2.1.B11 Thermococcus kodakarensis Q5JIA8 and Q5JIA9 Q5JIA8: alpha-subunit, Q5JIA9: beta-subunit
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.2.1.B10 recombinant TK2127/TK0943 protein Thermococcus kodakarensis
6.2.1.B11 recombinant TK0944/TK0943 protein Thermococcus kodakarensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.B10 ATP + 2-(imidazol-4-yl)acetate + CoA the enzyme is involved in catabolism of histidine Thermococcus kodakarensis ADP + phosphate + 2-(imidazol-4-yl)acetyl-CoA
-
 ?
6.2.1.B10 ATP + 2-(imidazol-4-yl)acetate + CoA the TK2127/TK0943 protein exhibits a strict preference for 2-(imidazol-4-yl)acetate, the metabolite expected in the degradation of histidine Thermococcus kodakarensis ADP + phosphate + 2-(imidazol-4-yl)acetyl-CoA
-
 ?
6.2.1.B11 ATP + 2-(4-hydroxyphenyl)acetate + CoA the enzyme accommodates a broad range of acids that correspond to those generated in the oxidative metabolism of Ala, Val, Leu, Ile, Met, Phe, and Cys Thermococcus kodakarensis ADP + phosphate + 2-(4-hydroxyphenyl)acetyl-CoA
-
 ?
6.2.1.B11 ATP + 2-(indol-3-yl)acetate + CoA low activity. Low activity. The enzyme accommodates a broad range of acids that correspond to those generated in the oxidative metabolism of Ala, Val, Leu, Ile, Met, Phe, and Cys Thermococcus kodakarensis ADP + phosphate + 2-(indol-3-yl)acetyl-CoA
-
 ?
6.2.1.B11 ATP + 2-methylbutyrate + CoA the enzyme accommodates a broad range of acids that correspond to those generated in the oxidative metabolism of Ala, Val, Leu, Ile, Met, Phe, and Cys Thermococcus kodakarensis ADP + phosphate + 2-methylbutyryl-CoA
-
 ?
6.2.1.B11 ATP + 3-methylthiopropionate + CoA the enzyme accommodates a broad range of acids that correspond to those generated in the oxidative metabolism of Ala, Val, Leu, Ile, Met, Phe, and Cys Thermococcus kodakarensis ADP + phosphate + 3-methylthiopropionyl-CoA
-
 ?
6.2.1.B11 ATP + 4-aminobutyrate + CoA low activity. The enzyme accommodates a broad range of acids that correspond to those generated in the oxidative metabolism of Ala, Val, Leu, Ile, Met, Phe, and Cys Thermococcus kodakarensis ADP + phosphate + 4-aminobutyryl-CoA
-
 ?
6.2.1.B11 ATP + acetate + CoA the enzyme accommodates a broad range of acids that correspond to those generated in the oxidative metabolism of Ala, Val, Leu, Ile, Met, Phe, and Cys Thermococcus kodakarensis ADP + phosphate + acetyl-CoA
-
 ?
6.2.1.B11 ATP + glycolate + CoA low activity. The enzyme accommodates a broad range of acids that correspond to those generated in the oxidative metabolism of Ala, Val, Leu, Ile, Met, Phe, and Cys Thermococcus kodakarensis ADP + phosphate + glycolyl-CoA
-
 ?
6.2.1.B11 ATP + isobutyrate + CoA the enzyme accommodates a broad range of acids that correspond to those generated in the oxidative metabolism of Ala, Val, Leu, Ile, Met, Phe, and Cys Thermococcus kodakarensis ADP + phosphate + isobutyryl-CoA
-
 ?
6.2.1.B11 ATP + isovalerate + CoA the enzyme accommodates a broad range of acids that correspond to those generated in the oxidative metabolism of Ala, Val, Leu, Ile, Met, Phe, and Cys Thermococcus kodakarensis ADP + phosphate + isovaleryl-CoA
-
 ?
6.2.1.B11 ATP + lactate + CoA low activity. The enzyme accommodates a broad range of acids that correspond to those generated in the oxidative metabolism of Ala, Val, Leu, Ile, Met, Phe, and Cys Thermococcus kodakarensis ADP + phosphate + lactoyl-CoA
-
 ?
6.2.1.B11 ATP + phenylacetate + CoA the enzyme accommodates a broad range of acids that correspond to those generated in the oxidative metabolism of Ala, Val, Leu, Ile, Met, Phe, and Cys Thermococcus kodakarensis ADP + phosphate + phenylacetyl-CoA
-
 ?
6.2.1.B11 ATP + thioglycolate + CoA the enzyme accommodates a broad range of acids that correspond to those generated in the oxidative metabolism of Ala, Val, Leu, Ile, Met, Phe, and Cys Thermococcus kodakarensis ADP + phosphate + thioglycolyl-CoA
-
 ?
6.2.1.B11 additional information activity with 2-(imidazol-4-yl)acetate is less than 5% of the activity with acetate Thermococcus kodakarensis ?
-
 ?

Subunits

EC Number Subunits Comment Organism
6.2.1.B10 tetramer 2 * 47645 + 2 * 27148, calculated from sequence Thermococcus kodakarensis
6.2.1.B11 tetramer 2 * 51861 (TK0944) + 2 * 27148 (TK0943), calculated from sequence Thermococcus kodakarensis

Synonyms

EC Number Synonyms Comment Organism
6.2.1.B10 2-(imidazol-4-yl)acetyl-CoA synthetase
-
 Thermococcus kodakarensis
6.2.1.B10 ADP-forming 2-(imidazol-4-yl)acetyl-CoA synthetase
-
 Thermococcus kodakarensis
6.2.1.B10 ICSTk
-
 Thermococcus kodakarensis
6.2.1.B10 TK2127/TK0943 protein
-
 Thermococcus kodakarensis
6.2.1.B11 ACSIIITk
-
 Thermococcus kodakarensis
6.2.1.B11 TK0944/TK0943 protein
-
 Thermococcus kodakarensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.2.1.B10 55
-
 assay at Thermococcus kodakarensis
6.2.1.B11 55
-
 assay at Thermococcus kodakarensis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6.2.1.B10 80
-
 half-life of the TK2127/TK0943 protein: 140 min Thermococcus kodakarensis
6.2.1.B10 90
-
 half-life of the TK2127/TK0943 protein: 120 min Thermococcus kodakarensis
6.2.1.B11 80
-
 half-life of the TK2127/TK0943 protein: 380 min Thermococcus kodakarensis
6.2.1.B11 90
-
 half-life of the TK2127/TK0943 protein: 170 min Thermococcus kodakarensis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.2.1.B10 additional information
-
 2-(imidazol-4-yl)acetate pH 6.5, 55°C, kinetic constant according to Adair-Pauling kinetic model: 37.8/s Thermococcus kodakarensis
6.2.1.B10 37.9
-
 ATP pH 6.5, 55°C Thermococcus kodakarensis
6.2.1.B10 39.6
-
 CoA pH 6.5, 55°C Thermococcus kodakarensis
6.2.1.B11 additional information
-
 thioglycolate pH 6.5, 55°C, kinetic constant according to Adair-Pauling kinetic model: 26.1/s Thermococcus kodakarensis
6.2.1.B11 additional information
-
 phenylacetate pH 6.5, 55°C, kinetic constant according to Adair-Pauling kinetic model: 40.6/s Thermococcus kodakarensis
6.2.1.B11 additional information
-
 acetate pH 6.5, 55°C, kinetic constant according to Adair-Pauling kinetic model: 49.3/s Thermococcus kodakarensis
6.2.1.B11 additional information
-
 Isobutyrate pH 6.5, 55°C, kinetic constant according to Adair-Pauling kinetic model: 50.9/s Thermococcus kodakarensis
6.2.1.B11 53.1
-
 CoA pH 6.5, 55°C Thermococcus kodakarensis
6.2.1.B11 53.7
-
 ATP pH 6.5, 55°C Thermococcus kodakarensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2.1.B10 6.5
-
 assay at Thermococcus kodakarensis
6.2.1.B11 6.5
-
 assay at Thermococcus kodakarensis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
6.2.1.B10 additional information
-
 2-(imidazol-4-yl)acetate pH 6.5, 55°C, kinetic constant according to Adair-Pauling kinetic model: 69.7/s* mM Thermococcus kodakarensis
6.2.1.B10 633
-
 CoA pH 6.5, 55°C Thermococcus kodakarensis
6.2.1.B10 1260
-
 ATP pH 6.5, 55°C Thermococcus kodakarensis
6.2.1.B11 additional information
-
 phenylacetate pH 6.5, 55°C, kinetic constant according to Adair-Pauling kinetic model: 123/s*mM Thermococcus kodakarensis
6.2.1.B11 additional information
-
 acetate pH 6.5, 55°C, kinetic constant according to Adair-Pauling kinetic model: 32.8/s*mM Thermococcus kodakarensis
6.2.1.B11 additional information
-
 Isobutyrate pH 6.5, 55°C, kinetic constant according to Adair-Pauling kinetic model: 385/s*mM Thermococcus kodakarensis
6.2.1.B11 additional information
-
 thioglycolate pH 6.5, 55°C, kinetic constant according to Adair-Pauling kinetic model: 81.0/s*mM Thermococcus kodakarensis
6.2.1.B11 965
-
 ATP pH 6.5, 55°C Thermococcus kodakarensis
6.2.1.B11 9490
-
 CoA pH 6.5, 55°C Thermococcus kodakarensis