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Literature summary extracted from

  • Li, P.; Wang, L.; Feng, L.
    Characterization of a novel Rieske-type alkane monooxygenase system in Pusillimonas sp. strain T7-7 (2013), J. Bacteriol., 195, 1892-1901.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.15.3 recombinant expression of His6-tagged enzyme system components in Escherichia coli strain BL21(DE3) Pusillimonas sp. T7-7

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.15.3 Ca2+
-
 Pusillimonas sp. T7-7
1.14.15.3 Co2+
-
 Pusillimonas sp. T7-7
1.14.15.3 Cu2+
-
 Pusillimonas sp. T7-7
1.14.15.3 EDTA
-
 Pusillimonas sp. T7-7
1.14.15.3 Mg2+
-
 Pusillimonas sp. T7-7
1.14.15.3 Mn2+
-
 Pusillimonas sp. T7-7
1.14.15.3 Ni2+
-
 Pusillimonas sp. T7-7
1.14.15.3 Pb2+
-
 Pusillimonas sp. T7-7
1.14.15.3 Zn2+
-
 Pusillimonas sp. T7-7

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.15.3 0.0182
-
 pentadecane pH 7.5, 30°C, recombinant alkane hydroxylase large subunit Pusillimonas sp. T7-7

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.15.3 Al3+ activates Pusillimonas sp. T7-7
1.14.15.3 Fe2+ required for the ferredoxin cofactor, activates Pusillimonas sp. T7-7
1.14.15.3 additional information no or poor effects by Na and K+ Pusillimonas sp. T7-7

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.15.3 53000
-
 3 * 53000, recombinant His6-tagged alkane hydroxylase large subunit, SDS-PAGE Pusillimonas sp. T7-7
1.14.15.3 160000
-
 recombinant His6-tagged alkane hydroxylase large subunit, native PAGE Pusillimonas sp. T7-7

Organism

EC Number Organism UniProt Comment Textmining
1.14.15.3 Pusillimonas sp. T7-7
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.15.3 recombinant His6-tagged enzyme system components from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Pusillimonas sp. T7-7

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.15.3 additional information strain T7-7 is able to utilize diesel oils, C5 to C30 alkane, as a sole carbon and energy source Pusillimonas sp. T7-7
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.15.3 1-hexadecene + reduced rubredoxin + O2
-
 Pusillimonas sp. T7-7 ? + oxidized rubredoxin + H2O
-
 ?
1.14.15.3 1-octadecene + reduced rubredoxin + O2
-
 Pusillimonas sp. T7-7 ? + oxidized rubredoxin + H2O
-
 ?
1.14.15.3 decane + reduced rubredoxin + O2
-
 Pusillimonas sp. T7-7 1-decanol + oxidized rubredoxin + H2O
-
 ?
1.14.15.3 dodecane + reduced rubredoxin + O2
-
 Pusillimonas sp. T7-7 1-dodecanol + oxidized rubredoxin + H2O
-
 ?
1.14.15.3 heptadecane + reduced rubredoxin + O2
-
 Pusillimonas sp. T7-7 1-heptadecanol + oxidized rubredoxin + H2O
-
 ?
1.14.15.3 hexadecane + reduced rubredoxin + O2
-
 Pusillimonas sp. T7-7 1-hexadecanol + oxidized rubredoxin + H2O
-
 ?
1.14.15.3 hexane + reduced rubredoxin + O2
-
 Pusillimonas sp. T7-7 1-hexanol + oxidized rubredoxin + H2O
-
 ?
1.14.15.3 methane sulfonic acid + reduced rubredoxin + O2
-
 Pusillimonas sp. T7-7 ? + oxidized rubredoxin + H2O
-
 ?
1.14.15.3 additional information substrate specificity of the alkane hydroxylase large subunit, overview. Poor activity with 1-tetradecanol, no activity with octacosane, benzene, sodium benzoate, toluene, catechol, biphenyl, 1-hexadecanol, 1-stearyl alcohol, isooctane, pristine, and squalane Pusillimonas sp. T7-7 ?
-
 ?
1.14.15.3 nitromethane + reduced rubredoxin + O2
-
 Pusillimonas sp. T7-7 ? + oxidized rubredoxin + H2O
-
 ?
1.14.15.3 octadecane + reduced rubredoxin + O2
-
 Pusillimonas sp. T7-7 1-octadecanol + oxidized rubredoxin + H2O
-
 ?
1.14.15.3 octane + reduced rubredoxin + O2
-
 Pusillimonas sp. T7-7 1-octanol + oxidized rubredoxin + H2O
-
 ?
1.14.15.3 pentadecane + reduced rubredoxin + O2 best substrate Pusillimonas sp. T7-7 1-pentadecanol + oxidized rubredoxin + H2O
-
 ?
1.14.15.3 pentane + reduced rubredoxin + O2
-
 Pusillimonas sp. T7-7 1-pentanol + oxidized rubredoxin + H2O
-
 ?
1.14.15.3 tetracosane + reduced rubredoxin + O2
-
 Pusillimonas sp. T7-7 1-tetracosanol + oxidized rubredoxin + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.15.3 More the alkane hydroxylase system consists of the alkane hydroxylase large subunit, the alkane hydroxylase small subunit, the NADH-dependent reductase subunit, and the ferredoxin subunit Pusillimonas sp. T7-7
1.14.15.3 trimer 3 * 53000, recombinant His6-tagged alkane hydroxylase large subunit, SDS-PAGE Pusillimonas sp. T7-7

Synonyms

EC Number Synonyms Comment Organism
1.14.15.3 alkane hydroxylase
-
 Pusillimonas sp. T7-7
1.14.15.3 Rieske-type alkane monooxygenase
-
 Pusillimonas sp. T7-7

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.15.3 30
-
 alkane hydroxylase large subunit Pusillimonas sp. T7-7

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.15.3 7.5
-
 alkane hydroxylase large subunit Pusillimonas sp. T7-7

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.15.3 Ferredoxin Rieske-type alkane monooxygenase with Rieske-type [2Fe-2S]2 center Pusillimonas sp. T7-7
1.14.15.3 NADH NADH-dependent alkane monooxygenase activity (C5 to C24), the alkane oxidation is coupled to NADH consumption Pusillimonas sp. T7-7

General Information

EC Number General Information Comment Organism
1.14.15.3 additional information the alkane hydroxylase system consists of the alkane hydroxylase large subunit, the alkane hydroxylase small subunit, the NADH-dependent reductase subunit, and the ferredoxin subunit. Analysis of the interaction between large and small subunits of the monooxygenase, overview Pusillimonas sp. T7-7