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Literature summary extracted from

  • Mlynek, G.; Sjoeblom, B.; Kostan, J.; Fuereder, S.; Maixner, F.; Gysel, K.; Furtmueller, P.G.; Obinger, C.; Wagner, M.; Daims, H.; Djinovic-Carugo, K.
    Unexpected diversity of chlorite dismutases: a catalytically efficient dimeric enzyme from Nitrobacter winogradskyi (2011), J. Bacteriol., 193, 2408-2417.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.49 DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of His-tagged enzyme in Escherichia coli strain Tuner (DE3) Nitrobacter winogradskyi

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.13.11.49 purified recombinant His-tagged enzyme, sitting-drop vapor diffusion technique and a nanodropdispensing robot, from 1.0 M Na2HPO4-NaH2PO4, pH 8.2, at 22°C, 1 week, X-ray diffraction structure determination and analysis at 2.10 A resolution Nitrobacter winogradskyi

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.49 additional information
-
 additional information Michaelis-Menten steady-state kinetics, overview Nitrobacter winogradskyi
1.13.11.49 0.09
-
 Chlorite pH 7.0, 30°C, recombinant enzyme Nitrobacter winogradskyi

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.13.11.49 cytoplasm
-
 Nitrobacter winogradskyi 5737
-
1.13.11.49 additional information the enzyme does not contain a signal peptide for protein translocation into the periplasm and that the cleaving of a signal peptide at the N terminus would severely disturb the structure of the N-terminal domain Nitrobacter winogradskyi
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.49 Fe2+ heme enzyme Nitrobacter winogradskyi

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.49 chlorite Nitrobacter winogradskyi
-
 chloride + O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.49 Nitrobacter winogradskyi Q3SPU6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.49 recombinant His-tagged enzyme from Escherichia coli strain Tuner (DE3) by nickel affinity chromatography and gel filtration Nitrobacter winogradskyi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.49 chlorite
-
 Nitrobacter winogradskyi chloride + O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.49 homodimer subunit interface and active site structures, structure modeling, overview Nitrobacter winogradskyi
1.13.11.49 More the Cld-like protein from the chemolithoautotrophic nitrite oxidizer Nitrobacter winogradskyi is significantly smaller than all previously known chlorite dismutases Nitrobacter winogradskyi

Synonyms

EC Number Synonyms Comment Organism
1.13.11.49 chlorite dismutase
-
 Nitrobacter winogradskyi
1.13.11.49 CLD
-
 Nitrobacter winogradskyi

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.11.49 30
-
 assay at Nitrobacter winogradskyi

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.13.11.49 190
-
 Chlorite pH 7.0, 30°C, recombinant enzyme Nitrobacter winogradskyi

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.49 7
-
 assay at Nitrobacter winogradskyi

Cofactor

EC Number Cofactor Comment Organism Structure
1.13.11.49 heme
-
 Nitrobacter winogradskyi

General Information

EC Number General Information Comment Organism
1.13.11.49 additional information despite a truncated N-terminal domain in each subunit, the dimeric enzyme is a highly efficient chlorite dismutase. Subunit interface and active site structures, structure modeling, overview Nitrobacter winogradskyi
1.13.11.49 physiological function the enzyme detoxifies the chlorite that results from chlorate respiration in bacteria and would otherwise accumulate in the cell and kill the organism. Nitrobacter has a limited chlorite resistance, which enables it to persist and even oxidize nitrite in the presence of low concentrations of chlorate Nitrobacter winogradskyi

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.13.11.49 2111
-
 Chlorite pH 7.0, 30°C, recombinant enzyme Nitrobacter winogradskyi