EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.3.1.157 | Ca2+ | 2 mM, inhibits D-glucosamine-1-phosphate N-acetyltransferase activity | Sulfurisphaera tokodaii | |
2.3.1.157 | Co2+ | 2 mM, inhibits D-glucosamine-1-phosphate N-acetyltransferase activity | Sulfurisphaera tokodaii | |
2.3.1.157 | Mg2+ | 2 mM, inhibits D-glucosamine-1-phosphate N-acetyltransferase activity | Sulfurisphaera tokodaii | |
2.3.1.157 | Mn2+ | 2 mM, inhibits D-glucosamine-1-phosphate N-acetyltransferase activity | Sulfurisphaera tokodaii | |
2.3.1.157 | Zn2+ | 2 mM, inhibits D-glucosamine-1-phosphate N-acetyltransferase activity | Sulfurisphaera tokodaii | |
2.3.1.276 | Mn2+ | 2 mM, 80% inhibition | Sulfurisphaera tokodaii | |
2.3.1.276 | Zn2+ | 2 mM, 75% inhibition | Sulfurisphaera tokodaii |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.157 | 0.59 | - |
alpha-D-glucosamine 1-phosphate | pH 7.5, 80°C | Sulfurisphaera tokodaii | |
2.3.1.157 | 0.63 | - |
acetyl-CoA | pH 7.5, 80°C | Sulfurisphaera tokodaii | |
2.3.1.276 | 0.63 | - |
acetyl-CoA | pH 7.5, 80°C | Sulfurisphaera tokodaii | |
2.3.1.276 | 1.71 | - |
alpha-D-galactosamine 1-phosphate | pH 7.5, 80°C | Sulfurisphaera tokodaii |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.3.1.157 | additional information | no metal ion required | Sulfurisphaera tokodaii | |
2.3.1.276 | Ca2+ | 2 mM, enhances galactosamine-1-phosphate N-acetyltransferase activity 2.1fold | Sulfurisphaera tokodaii | |
2.3.1.276 | Co2+ | 2 mM, enhances galactosamine-1-phosphate N-acetyltransferase activity 1.2fold | Sulfurisphaera tokodaii | |
2.3.1.276 | Mg2+ | 2 mM, enhances galactosamine-1-phosphate N-acetyltransferase activity 1.4fold | Sulfurisphaera tokodaii |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.157 | acetyl-CoA + alpha-D-glucosamine 1-phosphate | Sulfurisphaera tokodaii | because the ST0452 protein is capable of catalyzing the last two reactions (Ec 2.3.1.157 and EC 2.7.7.23 (UDP-N-acetylglucosamine diphosphorylase)) of the bacteria-type four-step biosynthesis pathway of UDP-GlcNAc from fructose 6-phosphate, the ST0452 protein plays an important role for the bacteria-type UDP-GlcNAc biosynthesis pathway in this archaeon | CoA + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
? | |
2.3.1.157 | acetyl-CoA + alpha-D-glucosamine 1-phosphate | Sulfurisphaera tokodaii 7 | because the ST0452 protein is capable of catalyzing the last two reactions (Ec 2.3.1.157 and EC 2.7.7.23 (UDP-N-acetylglucosamine diphosphorylase)) of the bacteria-type four-step biosynthesis pathway of UDP-GlcNAc from fructose 6-phosphate, the ST0452 protein plays an important role for the bacteria-type UDP-GlcNAc biosynthesis pathway in this archaeon | CoA + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
? | |
2.7.7.23 | UTP + N-acetyl-alpha-D-glucosamine 1-phosphate | Sulfurisphaera tokodaii | because the multifunctional ST0452 protein is capable of catalyzing the last two reactions (Ec 2.3.1.157 and EC 2.7.7.23 (UDP-N-acetylglucosamine diphosphorylase)) of the bacteria-type four-step biosynthesis pathway of UDP-GlcNAc from fructose 6-phosphate, the ST0452 protein plays an important role for the bacteria-type UDP-GlcNAc biosynthesis pathway in this archaeon | diphosphate + UDP-N-acetyl-alpha-D-glucosamine | - |
? | |
2.7.7.23 | UTP + N-acetyl-alpha-D-glucosamine 1-phosphate | Sulfurisphaera tokodaii 7 | because the multifunctional ST0452 protein is capable of catalyzing the last two reactions (Ec 2.3.1.157 and EC 2.7.7.23 (UDP-N-acetylglucosamine diphosphorylase)) of the bacteria-type four-step biosynthesis pathway of UDP-GlcNAc from fructose 6-phosphate, the ST0452 protein plays an important role for the bacteria-type UDP-GlcNAc biosynthesis pathway in this archaeon | diphosphate + UDP-N-acetyl-alpha-D-glucosamine | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.1.157 | Sulfurisphaera tokodaii | Q975F9 | - |
- |
2.3.1.157 | Sulfurisphaera tokodaii 7 | Q975F9 | - |
- |
2.3.1.276 | Sulfurisphaera tokodaii | Q975F9 | - |
- |
2.3.1.276 | Sulfurisphaera tokodaii 7 | Q975F9 | - |
- |
2.7.7.23 | Sulfurisphaera tokodaii | Q975F9 | - |
- |
2.7.7.23 | Sulfurisphaera tokodaii 7 | Q975F9 | - |
- |
2.7.7.83 | Sulfurisphaera tokodaii | Q975F9 | - |
- |
2.7.7.83 | Sulfurisphaera tokodaii 7 | Q975F9 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.3.1.157 | - |
Sulfurisphaera tokodaii |
2.3.1.276 | - |
Sulfurisphaera tokodaii |
2.7.7.23 | - |
Sulfurisphaera tokodaii |
2.7.7.83 | - |
Sulfurisphaera tokodaii |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
2.3.1.157 | acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + N-acetyl-alpha-D-glucosamine 1-phosphate | the multifunctional enzyme from Sulfolobus tokodaii {7} is also active with acetyl-CoA + alpha-D-galactosamine 1-phosphate (galactosamine-1-phosphate N-acetyltransferase), UTP + N-acetylglucosamine 1-phosphate (EC 2.7.7.23, UDP-N-acetylglucosamine diphosphorylase) and UTP + N-acetyl-alpha-D-galactosamine 1-phosphate (EC 2.7.7.83, UDP-N-acetylgalactosamine diphosphorylase) | Sulfurisphaera tokodaii |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.3.1.157 | 75.34 | - |
pH 7.5, 80°C, substrates: acetyl-CoA + alpha-D-glucosamine 1-phosphate | Sulfurisphaera tokodaii |
2.3.1.276 | 38.4 | - |
pH 7.5, 80°C, substrates: acetyl-CoA + alpha-D-galactosamine 1-phosphate | Sulfurisphaera tokodaii |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.157 | acetyl-CoA + alpha-D-glucosamine 1-phosphate | because the ST0452 protein is capable of catalyzing the last two reactions (Ec 2.3.1.157 and EC 2.7.7.23 (UDP-N-acetylglucosamine diphosphorylase)) of the bacteria-type four-step biosynthesis pathway of UDP-GlcNAc from fructose 6-phosphate, the ST0452 protein plays an important role for the bacteria-type UDP-GlcNAc biosynthesis pathway in this archaeon | Sulfurisphaera tokodaii | CoA + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
? | |
2.3.1.157 | acetyl-CoA + alpha-D-glucosamine 1-phosphate | no activity with alpha-D-glucosamine 6-phosphate. The multifunctional enzyme is also active with acetyl-CoA + alpha-D-galactosamine 1-phosphate (galactosamine-1-phosphate N-acetyltransferase), UTP + N-acetylglucosamine 1-phosphate (EC 2.7.7.23, UDP-N-acetylglucosamine diphosphorylase) and UTP + N-acetyl-alpha-D-galactosamine 1-phosphate (EC 2.7.7.83, UDP-N-acetylgalactosamine diphosphorylase) | Sulfurisphaera tokodaii | CoA + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
? | |
2.3.1.157 | acetyl-CoA + alpha-D-glucosamine 1-phosphate | because the ST0452 protein is capable of catalyzing the last two reactions (Ec 2.3.1.157 and EC 2.7.7.23 (UDP-N-acetylglucosamine diphosphorylase)) of the bacteria-type four-step biosynthesis pathway of UDP-GlcNAc from fructose 6-phosphate, the ST0452 protein plays an important role for the bacteria-type UDP-GlcNAc biosynthesis pathway in this archaeon | Sulfurisphaera tokodaii 7 | CoA + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
? | |
2.3.1.157 | acetyl-CoA + alpha-D-glucosamine 1-phosphate | no activity with alpha-D-glucosamine 6-phosphate. The multifunctional enzyme is also active with acetyl-CoA + alpha-D-galactosamine 1-phosphate (galactosamine-1-phosphate N-acetyltransferase), UTP + N-acetylglucosamine 1-phosphate (EC 2.7.7.23, UDP-N-acetylglucosamine diphosphorylase) and UTP + N-acetyl-alpha-D-galactosamine 1-phosphate (EC 2.7.7.83, UDP-N-acetylgalactosamine diphosphorylase) | Sulfurisphaera tokodaii 7 | CoA + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
? | |
2.3.1.276 | acetyl-CoA + alpha-D-galactosamine 1-phosphate | no activity with alpha-D-glucosamine 6-phosphate | Sulfurisphaera tokodaii | CoA + N-acetyl-alpha-D-galactosamine 1-phosphate | - |
? | |
2.7.7.23 | UTP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
Sulfurisphaera tokodaii | diphosphate + UDP-N-acetyl-alpha-D-glucosamine | - |
? | |
2.7.7.23 | UTP + N-acetyl-alpha-D-glucosamine 1-phosphate | because the multifunctional ST0452 protein is capable of catalyzing the last two reactions (Ec 2.3.1.157 and EC 2.7.7.23 (UDP-N-acetylglucosamine diphosphorylase)) of the bacteria-type four-step biosynthesis pathway of UDP-GlcNAc from fructose 6-phosphate, the ST0452 protein plays an important role for the bacteria-type UDP-GlcNAc biosynthesis pathway in this archaeon | Sulfurisphaera tokodaii | diphosphate + UDP-N-acetyl-alpha-D-glucosamine | - |
? | |
2.7.7.23 | UTP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
Sulfurisphaera tokodaii 7 | diphosphate + UDP-N-acetyl-alpha-D-glucosamine | - |
? | |
2.7.7.23 | UTP + N-acetyl-alpha-D-glucosamine 1-phosphate | because the multifunctional ST0452 protein is capable of catalyzing the last two reactions (Ec 2.3.1.157 and EC 2.7.7.23 (UDP-N-acetylglucosamine diphosphorylase)) of the bacteria-type four-step biosynthesis pathway of UDP-GlcNAc from fructose 6-phosphate, the ST0452 protein plays an important role for the bacteria-type UDP-GlcNAc biosynthesis pathway in this archaeon | Sulfurisphaera tokodaii 7 | diphosphate + UDP-N-acetyl-alpha-D-glucosamine | - |
? | |
2.7.7.83 | diphosphate + UDP-N-acetyl-alpha-D-galactosamine | - |
Sulfurisphaera tokodaii | UTP + N-acetyl-alpha-D-galactosamine 1-phosphate | - |
? | |
2.7.7.83 | diphosphate + UDP-N-acetyl-alpha-D-galactosamine | - |
Sulfurisphaera tokodaii 7 | UTP + N-acetyl-alpha-D-galactosamine 1-phosphate | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.3.1.157 | 80 | - |
assay at | Sulfurisphaera tokodaii |
2.3.1.276 | 80 | - |
assay at | Sulfurisphaera tokodaii |
2.7.7.23 | 80 | - |
assay at | Sulfurisphaera tokodaii |
2.7.7.83 | 80 | - |
assay at | Sulfurisphaera tokodaii |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.157 | 123.2 | - |
alpha-D-glucosamine 1-phosphate | pH 7.5, 80°C | Sulfurisphaera tokodaii | |
2.3.1.276 | 69.7 | - |
alpha-D-galactosamine 1-phosphate | pH 7.5, 80°C | Sulfurisphaera tokodaii |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.3.1.157 | 7.5 | - |
assay at | Sulfurisphaera tokodaii |
2.3.1.276 | 7.5 | - |
assay at | Sulfurisphaera tokodaii |
2.7.7.23 | 7.5 | - |
assay at | Sulfurisphaera tokodaii |
2.7.7.83 | 7.5 | - |
assay at | Sulfurisphaera tokodaii |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.3.1.157 | physiological function | because the ST0452 protein is capable of catalyzing the last two reactions (Ec 2.3.1.157 and EC 2.7.7.23 (UDP-N-acetylglucosamine diphosphorylase)) of the bacteria-type four-step biosynthesis pathway of UDP-GlcNAc from fructose 6-phosphate, the ST0452 protein plays an important role for the bacteria-type UDP-GlcNAc biosynthesis pathway in this archaeon | Sulfurisphaera tokodaii |
2.3.1.276 | physiological function | because the multifunctional ST0452 protein is capable of catalyzing the last two reactions (Ec 2.3.1.157 and EC 2.7.7.23 (UDP-N-acetylglucosamine diphosphorylase)) of the bacteria-type four-step biosynthesis pathway of UDP-alpha-D-glucosamine from fructose 6-phosphate, the ST0452 protein plays an important role for the bacteria-type UDP-alpha-D-glucosamine biosynthesis pathway in this archaeon | Sulfurisphaera tokodaii |
2.7.7.23 | physiological function | because the multifunctional ST0452 protein is capable of catalyzing the last two reactions (Ec 2.3.1.157 and EC 2.7.7.23 (UDP-N-acetylglucosamine diphosphorylase)) of the bacteria-type four-step biosynthesis pathway of UDP-GlcNAc from fructose 6-phosphate, the ST0452 protein plays an important role for the bacteria-type UDP-GlcNAc biosynthesis pathway in this archaeon | Sulfurisphaera tokodaii |
2.7.7.83 | physiological function | because the multifunctional ST0452 protein is capable of catalyzing the last two reactions (Ec 2.3.1.157 and EC 2.7.7.23 (UDP-N-acetylglucosamine diphosphorylase)) of the bacteria-type four-step biosynthesis pathway of UDP-GlcNAc from fructose 6-phosphate, the ST0452 protein plays an important role for the bacteria-type UDP-GlcNAc biosynthesis pathway in this archaeon | Sulfurisphaera tokodaii |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.157 | 211 | - |
alpha-D-glucosamine 1-phosphate | pH 7.5, 80°C | Sulfurisphaera tokodaii | |
2.3.1.276 | 40.8 | - |
alpha-D-galactosamine 1-phosphate | pH 7.5, 80°C | Sulfurisphaera tokodaii |