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Literature summary extracted from

  • Zhang, Z.; Akutsu, J.; Kawarabayasi, Y.
    Identification of novel acetyltransferase activity on the thermostable protein ST0452 from Sulfolobus tokodaii strain 7 (2010), J. Bacteriol., 192, 3287-3293.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.157 Ca2+ 2 mM, inhibits D-glucosamine-1-phosphate N-acetyltransferase activity Sulfurisphaera tokodaii
2.3.1.157 Co2+ 2 mM, inhibits D-glucosamine-1-phosphate N-acetyltransferase activity Sulfurisphaera tokodaii
2.3.1.157 Mg2+ 2 mM, inhibits D-glucosamine-1-phosphate N-acetyltransferase activity Sulfurisphaera tokodaii
2.3.1.157 Mn2+ 2 mM, inhibits D-glucosamine-1-phosphate N-acetyltransferase activity Sulfurisphaera tokodaii
2.3.1.157 Zn2+ 2 mM, inhibits D-glucosamine-1-phosphate N-acetyltransferase activity Sulfurisphaera tokodaii
2.3.1.276 Mn2+ 2 mM, 80% inhibition Sulfurisphaera tokodaii
2.3.1.276 Zn2+ 2 mM, 75% inhibition Sulfurisphaera tokodaii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.157 0.59
-
alpha-D-glucosamine 1-phosphate pH 7.5, 80°C Sulfurisphaera tokodaii
2.3.1.157 0.63
-
acetyl-CoA pH 7.5, 80°C Sulfurisphaera tokodaii
2.3.1.276 0.63
-
acetyl-CoA pH 7.5, 80°C Sulfurisphaera tokodaii
2.3.1.276 1.71
-
alpha-D-galactosamine 1-phosphate pH 7.5, 80°C Sulfurisphaera tokodaii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.1.157 additional information no metal ion required Sulfurisphaera tokodaii
2.3.1.276 Ca2+ 2 mM, enhances galactosamine-1-phosphate N-acetyltransferase activity 2.1fold Sulfurisphaera tokodaii
2.3.1.276 Co2+ 2 mM, enhances galactosamine-1-phosphate N-acetyltransferase activity 1.2fold Sulfurisphaera tokodaii
2.3.1.276 Mg2+ 2 mM, enhances galactosamine-1-phosphate N-acetyltransferase activity 1.4fold Sulfurisphaera tokodaii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.157 acetyl-CoA + alpha-D-glucosamine 1-phosphate Sulfurisphaera tokodaii because the ST0452 protein is capable of catalyzing the last two reactions (Ec 2.3.1.157 and EC 2.7.7.23 (UDP-N-acetylglucosamine diphosphorylase)) of the bacteria-type four-step biosynthesis pathway of UDP-GlcNAc from fructose 6-phosphate, the ST0452 protein plays an important role for the bacteria-type UDP-GlcNAc biosynthesis pathway in this archaeon CoA + N-acetyl-alpha-D-glucosamine 1-phosphate
-
?
2.3.1.157 acetyl-CoA + alpha-D-glucosamine 1-phosphate Sulfurisphaera tokodaii 7 because the ST0452 protein is capable of catalyzing the last two reactions (Ec 2.3.1.157 and EC 2.7.7.23 (UDP-N-acetylglucosamine diphosphorylase)) of the bacteria-type four-step biosynthesis pathway of UDP-GlcNAc from fructose 6-phosphate, the ST0452 protein plays an important role for the bacteria-type UDP-GlcNAc biosynthesis pathway in this archaeon CoA + N-acetyl-alpha-D-glucosamine 1-phosphate
-
?
2.7.7.23 UTP + N-acetyl-alpha-D-glucosamine 1-phosphate Sulfurisphaera tokodaii because the multifunctional ST0452 protein is capable of catalyzing the last two reactions (Ec 2.3.1.157 and EC 2.7.7.23 (UDP-N-acetylglucosamine diphosphorylase)) of the bacteria-type four-step biosynthesis pathway of UDP-GlcNAc from fructose 6-phosphate, the ST0452 protein plays an important role for the bacteria-type UDP-GlcNAc biosynthesis pathway in this archaeon diphosphate + UDP-N-acetyl-alpha-D-glucosamine
-
?
2.7.7.23 UTP + N-acetyl-alpha-D-glucosamine 1-phosphate Sulfurisphaera tokodaii 7 because the multifunctional ST0452 protein is capable of catalyzing the last two reactions (Ec 2.3.1.157 and EC 2.7.7.23 (UDP-N-acetylglucosamine diphosphorylase)) of the bacteria-type four-step biosynthesis pathway of UDP-GlcNAc from fructose 6-phosphate, the ST0452 protein plays an important role for the bacteria-type UDP-GlcNAc biosynthesis pathway in this archaeon diphosphate + UDP-N-acetyl-alpha-D-glucosamine
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.157 Sulfurisphaera tokodaii Q975F9
-
-
2.3.1.157 Sulfurisphaera tokodaii 7 Q975F9
-
-
2.3.1.276 Sulfurisphaera tokodaii Q975F9
-
-
2.3.1.276 Sulfurisphaera tokodaii 7 Q975F9
-
-
2.7.7.23 Sulfurisphaera tokodaii Q975F9
-
-
2.7.7.23 Sulfurisphaera tokodaii 7 Q975F9
-
-
2.7.7.83 Sulfurisphaera tokodaii Q975F9
-
-
2.7.7.83 Sulfurisphaera tokodaii 7 Q975F9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.157
-
Sulfurisphaera tokodaii
2.3.1.276
-
Sulfurisphaera tokodaii
2.7.7.23
-
Sulfurisphaera tokodaii
2.7.7.83
-
Sulfurisphaera tokodaii

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.1.157 acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + N-acetyl-alpha-D-glucosamine 1-phosphate the multifunctional enzyme from Sulfolobus tokodaii {7} is also active with acetyl-CoA + alpha-D-galactosamine 1-phosphate (galactosamine-1-phosphate N-acetyltransferase), UTP + N-acetylglucosamine 1-phosphate (EC 2.7.7.23, UDP-N-acetylglucosamine diphosphorylase) and UTP + N-acetyl-alpha-D-galactosamine 1-phosphate (EC 2.7.7.83, UDP-N-acetylgalactosamine diphosphorylase) Sulfurisphaera tokodaii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.157 75.34
-
pH 7.5, 80°C, substrates: acetyl-CoA + alpha-D-glucosamine 1-phosphate Sulfurisphaera tokodaii
2.3.1.276 38.4
-
pH 7.5, 80°C, substrates: acetyl-CoA + alpha-D-galactosamine 1-phosphate Sulfurisphaera tokodaii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.157 acetyl-CoA + alpha-D-glucosamine 1-phosphate because the ST0452 protein is capable of catalyzing the last two reactions (Ec 2.3.1.157 and EC 2.7.7.23 (UDP-N-acetylglucosamine diphosphorylase)) of the bacteria-type four-step biosynthesis pathway of UDP-GlcNAc from fructose 6-phosphate, the ST0452 protein plays an important role for the bacteria-type UDP-GlcNAc biosynthesis pathway in this archaeon Sulfurisphaera tokodaii CoA + N-acetyl-alpha-D-glucosamine 1-phosphate
-
?
2.3.1.157 acetyl-CoA + alpha-D-glucosamine 1-phosphate no activity with alpha-D-glucosamine 6-phosphate. The multifunctional enzyme is also active with acetyl-CoA + alpha-D-galactosamine 1-phosphate (galactosamine-1-phosphate N-acetyltransferase), UTP + N-acetylglucosamine 1-phosphate (EC 2.7.7.23, UDP-N-acetylglucosamine diphosphorylase) and UTP + N-acetyl-alpha-D-galactosamine 1-phosphate (EC 2.7.7.83, UDP-N-acetylgalactosamine diphosphorylase) Sulfurisphaera tokodaii CoA + N-acetyl-alpha-D-glucosamine 1-phosphate
-
?
2.3.1.157 acetyl-CoA + alpha-D-glucosamine 1-phosphate because the ST0452 protein is capable of catalyzing the last two reactions (Ec 2.3.1.157 and EC 2.7.7.23 (UDP-N-acetylglucosamine diphosphorylase)) of the bacteria-type four-step biosynthesis pathway of UDP-GlcNAc from fructose 6-phosphate, the ST0452 protein plays an important role for the bacteria-type UDP-GlcNAc biosynthesis pathway in this archaeon Sulfurisphaera tokodaii 7 CoA + N-acetyl-alpha-D-glucosamine 1-phosphate
-
?
2.3.1.157 acetyl-CoA + alpha-D-glucosamine 1-phosphate no activity with alpha-D-glucosamine 6-phosphate. The multifunctional enzyme is also active with acetyl-CoA + alpha-D-galactosamine 1-phosphate (galactosamine-1-phosphate N-acetyltransferase), UTP + N-acetylglucosamine 1-phosphate (EC 2.7.7.23, UDP-N-acetylglucosamine diphosphorylase) and UTP + N-acetyl-alpha-D-galactosamine 1-phosphate (EC 2.7.7.83, UDP-N-acetylgalactosamine diphosphorylase) Sulfurisphaera tokodaii 7 CoA + N-acetyl-alpha-D-glucosamine 1-phosphate
-
?
2.3.1.276 acetyl-CoA + alpha-D-galactosamine 1-phosphate no activity with alpha-D-glucosamine 6-phosphate Sulfurisphaera tokodaii CoA + N-acetyl-alpha-D-galactosamine 1-phosphate
-
?
2.7.7.23 UTP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
Sulfurisphaera tokodaii diphosphate + UDP-N-acetyl-alpha-D-glucosamine
-
?
2.7.7.23 UTP + N-acetyl-alpha-D-glucosamine 1-phosphate because the multifunctional ST0452 protein is capable of catalyzing the last two reactions (Ec 2.3.1.157 and EC 2.7.7.23 (UDP-N-acetylglucosamine diphosphorylase)) of the bacteria-type four-step biosynthesis pathway of UDP-GlcNAc from fructose 6-phosphate, the ST0452 protein plays an important role for the bacteria-type UDP-GlcNAc biosynthesis pathway in this archaeon Sulfurisphaera tokodaii diphosphate + UDP-N-acetyl-alpha-D-glucosamine
-
?
2.7.7.23 UTP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
Sulfurisphaera tokodaii 7 diphosphate + UDP-N-acetyl-alpha-D-glucosamine
-
?
2.7.7.23 UTP + N-acetyl-alpha-D-glucosamine 1-phosphate because the multifunctional ST0452 protein is capable of catalyzing the last two reactions (Ec 2.3.1.157 and EC 2.7.7.23 (UDP-N-acetylglucosamine diphosphorylase)) of the bacteria-type four-step biosynthesis pathway of UDP-GlcNAc from fructose 6-phosphate, the ST0452 protein plays an important role for the bacteria-type UDP-GlcNAc biosynthesis pathway in this archaeon Sulfurisphaera tokodaii 7 diphosphate + UDP-N-acetyl-alpha-D-glucosamine
-
?
2.7.7.83 diphosphate + UDP-N-acetyl-alpha-D-galactosamine
-
Sulfurisphaera tokodaii UTP + N-acetyl-alpha-D-galactosamine 1-phosphate
-
?
2.7.7.83 diphosphate + UDP-N-acetyl-alpha-D-galactosamine
-
Sulfurisphaera tokodaii 7 UTP + N-acetyl-alpha-D-galactosamine 1-phosphate
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.157 80
-
assay at Sulfurisphaera tokodaii
2.3.1.276 80
-
assay at Sulfurisphaera tokodaii
2.7.7.23 80
-
assay at Sulfurisphaera tokodaii
2.7.7.83 80
-
assay at Sulfurisphaera tokodaii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.157 123.2
-
alpha-D-glucosamine 1-phosphate pH 7.5, 80°C Sulfurisphaera tokodaii
2.3.1.276 69.7
-
alpha-D-galactosamine 1-phosphate pH 7.5, 80°C Sulfurisphaera tokodaii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.157 7.5
-
assay at Sulfurisphaera tokodaii
2.3.1.276 7.5
-
assay at Sulfurisphaera tokodaii
2.7.7.23 7.5
-
assay at Sulfurisphaera tokodaii
2.7.7.83 7.5
-
assay at Sulfurisphaera tokodaii

General Information

EC Number General Information Comment Organism
2.3.1.157 physiological function because the ST0452 protein is capable of catalyzing the last two reactions (Ec 2.3.1.157 and EC 2.7.7.23 (UDP-N-acetylglucosamine diphosphorylase)) of the bacteria-type four-step biosynthesis pathway of UDP-GlcNAc from fructose 6-phosphate, the ST0452 protein plays an important role for the bacteria-type UDP-GlcNAc biosynthesis pathway in this archaeon Sulfurisphaera tokodaii
2.3.1.276 physiological function because the multifunctional ST0452 protein is capable of catalyzing the last two reactions (Ec 2.3.1.157 and EC 2.7.7.23 (UDP-N-acetylglucosamine diphosphorylase)) of the bacteria-type four-step biosynthesis pathway of UDP-alpha-D-glucosamine from fructose 6-phosphate, the ST0452 protein plays an important role for the bacteria-type UDP-alpha-D-glucosamine biosynthesis pathway in this archaeon Sulfurisphaera tokodaii
2.7.7.23 physiological function because the multifunctional ST0452 protein is capable of catalyzing the last two reactions (Ec 2.3.1.157 and EC 2.7.7.23 (UDP-N-acetylglucosamine diphosphorylase)) of the bacteria-type four-step biosynthesis pathway of UDP-GlcNAc from fructose 6-phosphate, the ST0452 protein plays an important role for the bacteria-type UDP-GlcNAc biosynthesis pathway in this archaeon Sulfurisphaera tokodaii
2.7.7.83 physiological function because the multifunctional ST0452 protein is capable of catalyzing the last two reactions (Ec 2.3.1.157 and EC 2.7.7.23 (UDP-N-acetylglucosamine diphosphorylase)) of the bacteria-type four-step biosynthesis pathway of UDP-GlcNAc from fructose 6-phosphate, the ST0452 protein plays an important role for the bacteria-type UDP-GlcNAc biosynthesis pathway in this archaeon Sulfurisphaera tokodaii

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.3.1.157 211
-
alpha-D-glucosamine 1-phosphate pH 7.5, 80°C Sulfurisphaera tokodaii
2.3.1.276 40.8
-
alpha-D-galactosamine 1-phosphate pH 7.5, 80°C Sulfurisphaera tokodaii