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Literature summary extracted from

  • Ding, H.T.; Liu, D.F.; Li, Z.L.; Du, Y.Q.; Xu, X.H.; Zhao, Y.H.
    Characterization of a thermally stable and organic solvent-adaptative NAD+-dependent formate dehydrogenase from Bacillus sp. F1 (2011), J. Appl. Microbiol., 111, 1075-1085.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.17.1.9 EDTA the enzyme activity is increased 12% over the control by 1 mM EDTA Bacillus sp. (in: Bacteria)

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.17.1.9 expressed in Escherichia coli BL21(DE3) cells Bacillus sp. (in: Bacteria)

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.17.1.9 Cd2+ complete inhibition at 1 mM Bacillus sp. (in: Bacteria)
1.17.1.9 Cu2+ 94.2% inhibition at 1 mM Bacillus sp. (in: Bacteria)
1.17.1.9 Fe3+ 83.5% inhibition at 1 mM Bacillus sp. (in: Bacteria)
1.17.1.9 additional information no notable effect on the enzyme activity is observed when adding NH4+ or HPO2- Bacillus sp. (in: Bacteria)
1.17.1.9 Ni2+ 12.4% inhibition at 1 mM Bacillus sp. (in: Bacteria)
1.17.1.9 Zn2+ 55.5% inhibition at 1 mM Bacillus sp. (in: Bacteria)

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.17.1.9 0.091
-
NAD+ in 100 mM Tris-HCl (pH 7.0), at 30°C Bacillus sp. (in: Bacteria)
1.17.1.9 3.5
-
NADP+ in 100 mM Tris-HCl (pH 7.0), at 30°C Bacillus sp. (in: Bacteria)
1.17.1.9 19.6
-
formate with NAD+ as cosubstrate, in 100 mM Tris-HCl (pH 7.0), at 30°C Bacillus sp. (in: Bacteria)
1.17.1.9 39.1
-
formate with NADP+ as cosubstrate, in 100 mM Tris-HCl (pH 7.0), at 30°C Bacillus sp. (in: Bacteria)

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.17.1.9 Ca2+ the enzyme activity is increased 13.8% over the control by 1 mM Ca2+ Bacillus sp. (in: Bacteria)
1.17.1.9 Co2+ the enzyme activity is increased 9.8% over the control by 1 mM Co2+ Bacillus sp. (in: Bacteria)
1.17.1.9 Fe2+ the enzyme activity is increased 49.4% over the control by 1 mM Fe2+ Bacillus sp. (in: Bacteria)
1.17.1.9 K+ the enzyme activity is increased 6.7% over the control by 1 mM K+ Bacillus sp. (in: Bacteria)
1.17.1.9 Li+ the enzyme activity is increased 13.8% over the control by 1 mM Li+ Bacillus sp. (in: Bacteria)
1.17.1.9 Mg2+ the enzyme activity is increased 7.6% over the control by 1 mM Mg2+ Bacillus sp. (in: Bacteria)
1.17.1.9 Mn2+ the enzyme activity is increased 29.8% over the control by 1 mM Mn2+ Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.17.1.9 44000
-
2 * 44000, calculated from amino acid sequence Bacillus sp. (in: Bacteria)
1.17.1.9 45000
-
2 * 45000, SDS-PAGE Bacillus sp. (in: Bacteria)

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.17.1.9 formate + NAD+ Bacillus sp. (in: Bacteria) the enzyme shows strict substrate specificity for formate CO2 + NADH + H+
-
?
1.17.1.9 formate + NAD+ Bacillus sp. (in: Bacteria) F1 the enzyme shows strict substrate specificity for formate CO2 + NADH + H+
-
?
1.17.1.9 formate + NADP+ Bacillus sp. (in: Bacteria)
-
CO2 + NADPH
-
?
1.17.1.9 formate + NADP+ Bacillus sp. (in: Bacteria) F1
-
CO2 + NADPH
-
?
1.17.1.9 additional information Bacillus sp. (in: Bacteria) the enzyme shows no activity when methanol, ethanol, formaldehyde, sodium acetate, sodium malate, sodium oxalate, sodium lactate, sodium succinate, sodium citrate and sodium nitrate are used as the sole substrate ?
-
?
1.17.1.9 additional information Bacillus sp. (in: Bacteria) F1 the enzyme shows no activity when methanol, ethanol, formaldehyde, sodium acetate, sodium malate, sodium oxalate, sodium lactate, sodium succinate, sodium citrate and sodium nitrate are used as the sole substrate ?
-
?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
1.17.1.9 3-methyl-1-butanol the enzyme retains 87% of the initial activity after incubating in 60% (v/v) 3-methyl-1-butanol for 1 h Bacillus sp. (in: Bacteria)
1.17.1.9 Acetone the enzyme retains nearly 100% of the initial activity after incubating with up to 60% (v/v) acetone Bacillus sp. (in: Bacteria)
1.17.1.9 dimethyl sulfoxide the enzyme retains nearly 100% of the initial activity after incubating with up to 20% (v/v) dimethyl sulfoxide Bacillus sp. (in: Bacteria)
1.17.1.9 Ethanol the enzyme retains nearly 100% of the initial activity after incubating with up to 20% (v/v) ethanol Bacillus sp. (in: Bacteria)
1.17.1.9 isopropanol the enzyme retains nearly 100% of the initial activity after incubating with up to 60% (v/v) isopropanol Bacillus sp. (in: Bacteria)
1.17.1.9 Methanol the enzyme retains nearly 100% of the initial activity after incubating with up to 10% (v/v) methanol Bacillus sp. (in: Bacteria)
1.17.1.9 n-hexane the enzyme retains nearly 100% of the initial activity after incubating with up to 20% (v/v) n-hexane Bacillus sp. (in: Bacteria)

Organism

EC Number Organism UniProt Comment Textmining
1.17.1.9 Bacillus sp. (in: Bacteria) D6CJK0
-
-
1.17.1.9 Bacillus sp. (in: Bacteria) F1 D6CJK0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.17.1.9 Ni-NTA resin column chromatography Bacillus sp. (in: Bacteria)

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.17.1.9 6.03
-
purified enzyme, in 100 mM Tris-HCl (pH 7.0), at 30°C Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.1.9 formate + NAD+ the enzyme shows strict substrate specificity for formate Bacillus sp. (in: Bacteria) CO2 + NADH + H+
-
?
1.17.1.9 formate + NAD+ the enzyme shows strict substrate specificity for formate Bacillus sp. (in: Bacteria) F1 CO2 + NADH + H+
-
?
1.17.1.9 formate + NADP+
-
Bacillus sp. (in: Bacteria) CO2 + NADPH
-
?
1.17.1.9 formate + NADP+
-
Bacillus sp. (in: Bacteria) F1 CO2 + NADPH
-
?
1.17.1.9 additional information the enzyme shows no activity when methanol, ethanol, formaldehyde, sodium acetate, sodium malate, sodium oxalate, sodium lactate, sodium succinate, sodium citrate and sodium nitrate are used as the sole substrate Bacillus sp. (in: Bacteria) ?
-
?
1.17.1.9 additional information the enzyme shows no activity when methanol, ethanol, formaldehyde, sodium acetate, sodium malate, sodium oxalate, sodium lactate, sodium succinate, sodium citrate and sodium nitrate are used as the sole substrate Bacillus sp. (in: Bacteria) F1 ?
-
?

Subunits

EC Number Subunits Comment Organism
1.17.1.9 homodimer 2 * 45000, SDS-PAGE Bacillus sp. (in: Bacteria)
1.17.1.9 homodimer 2 * 44000, calculated from amino acid sequence Bacillus sp. (in: Bacteria)

Synonyms

EC Number Synonyms Comment Organism
1.17.1.9 FDH
-
Bacillus sp. (in: Bacteria)
1.17.1.9 NAD+-dependent formate dehydrogenase
-
Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.17.1.9 50
-
-
Bacillus sp. (in: Bacteria)

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.17.1.9 25 60 enzyme activity increases linearly from 25 to 50°C and reaches the highest at 50°C. The enzyme activity at temperatures ranging from 35 to 60°C is always more than 60% of the maximum activity Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.17.1.9 50 60 the enzyme is stable at temperatures below 55°C for 30 min and retains 71% of the initial activity after incubation at 60°C for 30 min. The half-life at 60°C is 52.9 min Bacillus sp. (in: Bacteria)

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.17.1.9 1.07
-
NADP+ in 100 mM Tris-HCl (pH 7.0), at 30°C Bacillus sp. (in: Bacteria)
1.17.1.9 6.67
-
NAD+ in 100 mM Tris-HCl (pH 7.0), at 30°C Bacillus sp. (in: Bacteria)

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.17.1.9 6
-
-
Bacillus sp. (in: Bacteria)

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.17.1.9 5.5 9.5 the specific activity at pH ranging from 5.5 to 9.5 is always more than 77% of the maximum activity Bacillus sp. (in: Bacteria)

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.17.1.9 4.5 10.5 the enzyme retains more than 80% of the activities after incubating in buffers with different pH ranging from 4.5 to 10.5 for 1 h Bacillus sp. (in: Bacteria)

Cofactor

EC Number Cofactor Comment Organism Structure
1.17.1.9 NAD+ preferred cofactor Bacillus sp. (in: Bacteria)
1.17.1.9 NADP+
-
Bacillus sp. (in: Bacteria)

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.17.1.9 Bacillus sp. (in: Bacteria) calculated from amino acid sequence
-
6.9

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.17.1.9 0.306
-
NADP+ in 100 mM Tris-HCl (pH 7.0), at 30°C Bacillus sp. (in: Bacteria)
1.17.1.9 73.3
-
NAD+ in 100 mM Tris-HCl (pH 7.0), at 30°C Bacillus sp. (in: Bacteria)