EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.361 | expression in Escherichia coli | Bacillus subtilis |
2.6.1.104 | expression of the N-terminal hexahistidine-tagged protein in Escherichia coli | Bacillus subtilis |
3.1.3.92 | expression in 'Escherichia coli | Bacillus subtilis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.3.92 | 0.101 | - |
kanosamine 6-phosphate | pH not specified in the publication, temperature not specified in the publication | Bacillus subtilis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.6.1.104 | kanosamine 6-phosphate + 2-oxoglutarate | Bacillus subtilis | the enzyme is involved in a kanosamine biosynthesis pathway | 3-dehydro-D-glucose 6-phosphate + L-glutamate | - |
? | |
2.6.1.104 | kanosamine 6-phosphate + 2-oxoglutarate | Bacillus subtilis 168 | the enzyme is involved in a kanosamine biosynthesis pathway | 3-dehydro-D-glucose 6-phosphate + L-glutamate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.361 | Bacillus subtilis | - |
- |
- |
2.6.1.104 | Bacillus subtilis | O07566 | - |
- |
2.6.1.104 | Bacillus subtilis 168 | O07566 | - |
- |
3.1.3.92 | Bacillus subtilis | O07565 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.361 | D-glucose + NAD+ | poor substrate | Bacillus subtilis | 3-dehydro-D-glucose + NADH + H+ | - |
? | |
1.1.1.361 | D-glucose 6-phosphate + NAD+ | - |
Bacillus subtilis | 3-dehydro-D-glucose 6-phosphate + NADH + H+ | - |
? | |
1.1.1.361 | inositol + NAD+ | poor substrate | Bacillus subtilis | ? + NADH + H+ | - |
? | |
1.1.1.361 | additional information | no substrate: UDP-glucose | Bacillus subtilis | ? | - |
? | |
2.6.1.104 | kanosamine 6-phosphate + 2-oxoglutarate | - |
Bacillus subtilis | 3-dehydro-D-glucose 6-phosphate + L-glutamate | - |
? | |
2.6.1.104 | kanosamine 6-phosphate + 2-oxoglutarate | the enzyme is involved in a kanosamine biosynthesis pathway | Bacillus subtilis | 3-dehydro-D-glucose 6-phosphate + L-glutamate | - |
? | |
2.6.1.104 | kanosamine 6-phosphate + 2-oxoglutarate | - |
Bacillus subtilis 168 | 3-dehydro-D-glucose 6-phosphate + L-glutamate | - |
? | |
2.6.1.104 | kanosamine 6-phosphate + 2-oxoglutarate | the enzyme is involved in a kanosamine biosynthesis pathway | Bacillus subtilis 168 | 3-dehydro-D-glucose 6-phosphate + L-glutamate | - |
? | |
3.1.3.92 | D-glucosamine 6-phosphate + H2O | about 1% of the activity with kanosamine 6-phosphate | Bacillus subtilis | D-glucosamine + phosphate | - |
? | |
3.1.3.92 | kanosamine 6-phosphate + H2O | - |
Bacillus subtilis | kanosamine + phosphate | - |
? | |
3.1.3.92 | additional information | no substrates: glucose 6-phosphate, 4-nitrophenyl phosphate, 3,3'-neotrehalosediamine 6-phosphate, 3,3'-neotrehalosediamine 6'-phosphate, 3,3'-neotrehalosediamine 6,6'-bisphosphate, glucose 1-phosphate | Bacillus subtilis | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.361 | ntdC | - |
Bacillus subtilis |
1.1.1.361 | yhjJ | - |
Bacillus subtilis |
2.6.1.104 | 3-oxo-glucose-6-phosphate:glutamate aminotransferase | - |
Bacillus subtilis |
2.6.1.104 | ntdA | - |
Bacillus subtilis |
3.1.3.92 | ntdB | - |
Bacillus subtilis |
3.1.3.92 | yhjK | - |
Bacillus subtilis |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.3.92 | 32 | - |
kanosamine 6-phosphate | pH not specified in the publication, temperature not specified in the publication | Bacillus subtilis |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.361 | additional information | no cofactor: NADP+ | Bacillus subtilis | |
1.1.1.361 | NAD+ | highly specific | Bacillus subtilis | |
2.6.1.104 | pyridoxal 5'-phosphate | a pyridoxal-phosphate protein | Bacillus subtilis |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.1.1.361 | physiological function | the ntd operon is essential for biosynthesis of the unusual disaccharide 3,3'-neotrehalosadiamine. The enzymes catalyze the biosynthesis of kanosamine from D-glucose 6-phosphate. NtdC is a D-glucose-6-phosphate-3-dehydrogenase, NtdA is a pyridoxal phosphate-dependent 3-oxo-glucose-6-phosphate:glutamate aminotransferase, and NtdB is a kanosamine-6-phosphate phosphatase | Bacillus subtilis |
2.6.1.104 | physiological function | the enzyme is involved in a kanosamine biosynthesis pathway | Bacillus subtilis |
3.1.3.92 | physiological function | the ntd operon is essential for biosynthesis of the unusual disaccharide 3,3'-neotrehalosadiamine. The enzymes catalyze the biosynthesis of kanosamine from glucose 6-phosphate. NtdC is a glucose-6-phosphate 3-dehydrogenase, NtdA is a pyridoxal phosphate-dependent 3-oxo-glucose-6-phosphate:glutamate aminotransferase, and NtdB is a kanosamine-6-phosphate phosphatase | Bacillus subtilis |