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Literature summary extracted from

  • Kovinich, N.; Saleem, A.; Arnason, J.T.; Miki, B.
    Identification of two anthocyanidin reductase genes and three red-brown soybean accessions with reduced anthocyanidin reductase 1 mRNA, activity, and seed coat proanthocyanidin amounts (2012), J. Agric. Food Chem., 60, 574-584.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.77 recombinantly expressed in Escherichia coli Glycine max

Protein Variants

EC Number Protein Variants Comment Organism
1.3.1.77 N185I/G214A mutant shows minor reductions in activity. Calculated Km and Vmax values are not given as the enzymes are intensely inhibited by cyanidin concentrations above 0.1 mM Glycine max
1.3.1.77 V122A/G214A mutant shows minor reductions in activity. Calculated Km and Vmax values are not given as the enzymes are intensely inhibited by cyanidin concentrations above 0.1 mM Glycine max

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.1.77 39000
-
 SDS-PAGE, recombinant protein Glycine max

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.77 Glycine max
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.3.1.77 additional information ANR2 shows minor expression in all organs Glycine max
-
1.3.1.77 seed coat preferrently expressed Glycine max
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.77 (+)-epicatechin + 2 NADP+
-
 Glycine max cyanidin + 2 NADPH + 2 H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.3.1.77 ANR1
-
 Glycine max
1.3.1.77 ANR2
-
 Glycine max

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.1.77 45
-
 assay at Glycine max