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Literature summary extracted from

  • Li, C.; Bai, Y.; Li, S.; Chen, H.; Han, X.; Zhao, H.; Shao, J.; Park, S.U.; Wu, Q.
    Cloning, characterization, and activity analysis of a flavonol synthase gene FtFLS1 and its association with flavonoid content in tartary buckwheat (2012), J. Agric. Food Chem., 60, 5161-5168.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.20.6 gene FtFLS1, DNA and amino acid sequence determination and analysis, phylogenetic tree, functional expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Fagopyrum tataricum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.20.6 Fe2+ required Fagopyrum tataricum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.20.6 40000
-
 x * 40000, recombinant enzyme, SDS-PAGE Fagopyrum tataricum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.20.6 (2R/3R)-dihydrokaempferol + 2-oxoglutarate + O2 Fagopyrum tataricum
-
 kaempferol + succinate + CO2 + H2O
-
 ?
1.14.20.6 (2R/3R)-dihydroquercetin + 2-oxoglutarate + O2 Fagopyrum tataricum
-
 quercetin + succinate + CO2 + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.20.6 Fagopyrum tataricum F5BR59 gene FtFLS1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.20.6 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Fagopyrum tataricum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.20.6 flower
-
 Fagopyrum tataricum
-
1.14.20.6 flower bud
-
 Fagopyrum tataricum
-
1.14.20.6 leaf highest enzyme expression, especially high at initial bloom Fagopyrum tataricum
-
1.14.20.6 additional information the overall expression levels are the highest in leaves and flowers, the enzyme shows a moderate level in stems, and lower expression in flower buds and immature seeds Fagopyrum tataricum
-
1.14.20.6 seed
-
 Fagopyrum tataricum
-
1.14.20.6 stem
-
 Fagopyrum tataricum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.20.6 0.0053
-
 purified recombinant enzyme with substrate (2R/3R)-dihydrokaempferol, pH 5.0, 37°C Fagopyrum tataricum
1.14.20.6 0.019
-
 purified recombinant enzyme with substrate (2R/3R)-dihydroquercetin, pH 5.0, 37°C Fagopyrum tataricum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.20.6 (2R/3R)-dihydrokaempferol + 2-oxoglutarate + O2
-
 Fagopyrum tataricum kaempferol + succinate + CO2 + H2O
-
 ?
1.14.20.6 (2R/3R)-dihydroquercetin + 2-oxoglutarate + O2
-
 Fagopyrum tataricum quercetin + succinate + CO2 + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.20.6 ? x * 40000, recombinant enzyme, SDS-PAGE Fagopyrum tataricum

Synonyms

EC Number Synonyms Comment Organism
1.14.20.6 FLS1
-
 Fagopyrum tataricum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.20.6 37
-
 assay at Fagopyrum tataricum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.20.6 5
-
 assay at Fagopyrum tataricum

General Information

EC Number General Information Comment Organism
1.14.20.6 evolution the enzyme belongs to the 2-oxoglutarate-dependent dioxygenase family Fagopyrum tataricum
1.14.20.6 physiological function the enzyme controls a key step in flavonol biosynthesis and might play a key role in rutin biosynthesis Fagopyrum tataricum