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Literature summary extracted from

  • Yadav, P.; Yadav, M.; Yadav, K.; Sharma, J.; Singh, V.
    Purification of chloroperoxidase from Musa paradisiaca stem juice (2013), Int. J. Chem. Kinetics, 45, 92-100.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.11.1.10 azide
-
 Musa x paradisiaca

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.10 0.02
-
 monochlorodimedone in 50 mM phosphate buffer pH 2.75 at 30°C, with 20 mM KCl Musa x paradisiaca
1.11.1.10 0.089
-
 pyrogallol in 50 mM phosphate buffer pH 2.75 at 30°C, with 20 mM KCl Musa x paradisiaca
1.11.1.10 0.12
-
 H2O2 in 50 mM phosphate buffer pH 2.75 at 30°C, with 20 mM KCl Musa x paradisiaca

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.11.1.10 43000
-
 x * 43000, SDS-PAGE Musa x paradisiaca

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.10 Musa x paradisiaca
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.10 ultrafiltration and DEAE cellulose column chromatography Musa x paradisiaca

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.11.1.10 stem juice
-
 Musa x paradisiaca
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.11.1.10 0.27
-
 crude enzyme, at pH 2.75 and 30°C Musa x paradisiaca
1.11.1.10 2.25
-
 after 8.33fold purification, at pH 2.75 and 30°C Musa x paradisiaca

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.10 4-chloroaniline + chloride + H2O2
-
 Musa x paradisiaca ?
-
 ?
1.11.1.10 monochlorodimedone + chloride + H2O2
-
 Musa x paradisiaca dichlorodimedone + H2O
-
 ?
1.11.1.10 pyrogallol + chloride + H2O2
-
 Musa x paradisiaca ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.11.1.10 ? x * 43000, SDS-PAGE Musa x paradisiaca

Synonyms

EC Number Synonyms Comment Organism
1.11.1.10 chloroperoxidase
-
 Musa x paradisiaca
1.11.1.10 CPO
-
 Musa x paradisiaca

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.11.1.10 25
-
-
 Musa x paradisiaca

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.11.1.10 1.64
-
 monochlorodimedone in 50 mM phosphate buffer pH 2.75 at 30°C, with 20 mM KCl Musa x paradisiaca
1.11.1.10 26.56
-
 pyrogallol in 50 mM phosphate buffer pH 2.75 at 30°C, with 20 mM KCl Musa x paradisiaca

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.1.10 3
-
-
 Musa x paradisiaca

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.11.1.10 2.5 6 at pH 2.75 it loses 85% activity in 1 h, and it is completely inactivated in 24 h. At pH 4.4, the enzyme also loses activity with time and is completely inactivated after 2.5 h. However, the enzyme exposed to pH 6.0 has low activity but it remains constant for 24 h Musa x paradisiaca

Cofactor

EC Number Cofactor Comment Organism Structure
1.11.1.10 heme
-
 Musa x paradisiaca

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.11.1.10 82
-
 monochlorodimedone in 50 mM phosphate buffer pH 2.75 at 30°C, with 20 mM KCl Musa x paradisiaca
1.11.1.10 298.4
-
 pyrogallol in 50 mM phosphate buffer pH 2.75 at 30°C, with 20 mM KCl Musa x paradisiaca