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Literature summary extracted from

  • Sun, W.; Shen, Y.H.; Qi, D.W.; Xiang, Z.H.; Zhang, Z.
    Molecular cloning and characterization of ecdysone oxidase and 3-dehydroecdysone-3beta-reductase involved in the ecdysone inactivation pathway of silkworm, Bombyx mori (2012), Int. J. Biol. Sci., 8, 125-138.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.3.16 DNA and amino acid sequence determination and analysis, expression of His-tagged enzyme in Escherichia coli strain BL21 (DE3) Bombyx mori

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.3.16 72000
-
 x * 72000, SDS-PAGE Bombyx mori

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.3.16 ecdysone + O2 Bombyx mori inactivation of ecdysone 3-dehydroecdysone + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.16 Bombyx mori H6AGY0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.16 recombinant His-tagged enzyme from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography Bombyx mori

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.3.16 midgut also in midgut goblet cell cavities Bombyx mori
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.16 ecdysone + O2
-
 Bombyx mori 3-dehydroecdysone + H2O2
-
 ?
1.1.3.16 ecdysone + O2 inactivation of ecdysone Bombyx mori 3-dehydroecdysone + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.3.16 ? x * 72000, SDS-PAGE Bombyx mori

General Information

EC Number General Information Comment Organism
1.1.3.16 metabolism ecdysone is inactivated to 3-dehydroecdysone under ecdysone oxidase, followed by NAD(P)H-dependent irreversible reduction to 3-epiecdysteroid under 3-dehydroecdysone 3alpha-reductase. On the other hand, 3-dehydroecdysone undergoes reversible reduction to ecdysone by 3-dehydroecdysone 3beta-reductase in the hemolymph Bombyx mori