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Literature summary extracted from

  • Rajan, L.; Joseph, T.; Thampuran, N.; James, R.
    Functional characterization and sequence analysis of choline dehydrogenase from Escherichia coli (2010), Genet. Eng. Biotechnol. J., 2010, 0000.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.99.1 gene betA, DNA and amino acid sequence determination and analysis, sequence comparisons, expression in Escherichia coli strain M15/pREP4, subcloning in strain JM109 Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.99.1 61848
-
 x * 61848, sequence calculation Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
1.1.99.1 Escherichia coli A7LLT8 isolated from a finfish, gene betA
-
1.1.99.1 Escherichia coli TCJAR023 A7LLT8 isolated from a finfish, gene betA
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.99.1 recombnant enzyme from Escherichia coli strain M15 by ammonium sulfate fractionation and anion exchange chromatography Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.99.1 choline + phenazine methosulfate
-
 Escherichia coli betaine aldehyde + reduced phenazine methosulfate
-
 ?
1.1.99.1 choline + phenazine methosulfate
-
 Escherichia coli TCJAR023 betaine aldehyde + reduced phenazine methosulfate
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.99.1 ? x * 61848, sequence calculation Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.99.1 7
-
 assay at Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.99.1 phenazine methosulfate
-
 Escherichia coli

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.1.99.1 Escherichia coli sequence calculation
-
 5.45

General Information

EC Number General Information Comment Organism
1.1.99.1 metabolism in Escherichia coli, the biosynthetic pathway for the production of glycine betaine from choline involves choline dehydrogenase (betA), betaine aldehyde dehydrogenase (betB), a putative regulator (betI) and a choline transporter (betT), the genes are clustered in the bet operon Escherichia coli