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Literature summary extracted from

  • Seitz, E.M.; Brockman, J.P.; Sandler, S.J.; Clark, A.J.; Kowalczykowski, S.C.
    RadA protein is an archaeal RecA protein homolog that catalyzes DNA strand exchange (1998), Genes Dev., 12, 1248-1253.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.6.4.B7 ssDNA the RadA protein is a ssDNA-dependent ATPase Saccharolobus solfataricus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.4.B7 Mg2+ required Saccharolobus solfataricus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.4.B7 ATP + H2O Saccharolobus solfataricus the RadA protein is a DNA-dependent ATPase, forms a nucleoprotein filament on DNA, and catalyzes DNA pairing and strand exchange ADP + phosphate
-
 ?
3.6.4.B7 ATP + H2O Saccharolobus solfataricus P2 the RadA protein is a DNA-dependent ATPase, forms a nucleoprotein filament on DNA, and catalyzes DNA pairing and strand exchange ADP + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.6.4.B7 Saccharolobus solfataricus Q55075
-
-
3.6.4.B7 Saccharolobus solfataricus P2 Q55075
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.4.B7
-
 Saccharolobus solfataricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.4.B7 ATP + H2O the RadA protein is a DNA-dependent ATPase, forms a nucleoprotein filament on DNA, and catalyzes DNA pairing and strand exchange Saccharolobus solfataricus ADP + phosphate
-
 ?
3.6.4.B7 ATP + H2O the RadA protein is a ssDNA-dependent ATPase. ATP hydrolysis is less efficient in the presence of double-stranded DNA, and almost no hydrolysis occurs in the absence of DNA. It forms a nucleoprotein filament on DNA, and catalyzes DNA pairing and strand exchange Saccharolobus solfataricus ADP + phosphate
-
 ?
3.6.4.B7 ATP + H2O the RadA protein is a DNA-dependent ATPase, forms a nucleoprotein filament on DNA, and catalyzes DNA pairing and strand exchange Saccharolobus solfataricus P2 ADP + phosphate
-
 ?
3.6.4.B7 ATP + H2O the RadA protein is a ssDNA-dependent ATPase. ATP hydrolysis is less efficient in the presence of double-stranded DNA, and almost no hydrolysis occurs in the absence of DNA. It forms a nucleoprotein filament on DNA, and catalyzes DNA pairing and strand exchange Saccharolobus solfataricus P2 ADP + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.6.4.B7 RadA
-
 Saccharolobus solfataricus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.6.4.B7 65 85 DNA-dependent ATP hydrolysis occurrs exclusively at elevated temperatures (65°C, 75°C, 85°C), and very little hydrolysis occurrs at 37°C Saccharolobus solfataricus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.6.4.B7 0.02
-
 ATP pH 7.5, 37°C, 6 mM MgCl2, addition of poly(dT) Saccharolobus solfataricus
3.6.4.B7 0.12
-
 ATP pH 7.5, 65°C, 6 mM MgCl2, addition of poly(dT) Saccharolobus solfataricus
3.6.4.B7 0.14
-
 ATP pH 7.5, 75°C, 6 mM MgCl2, addition of poly(dT) Saccharolobus solfataricus
3.6.4.B7 0.17
-
 ATP pH 7.5, 85°C, 6 mM MgCl2, addition of poly(dT) Saccharolobus solfataricus