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Literature summary extracted from

  • Han, X.; Zhao, M.; Lu, L.; Liu, Y.
    Purification, characterization and decolorization of bilirubin oxidase from Myrothecium verrucaria 3.2190 (2012), Fungal Biol., 116, 863-871.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.3.3.5 additional information the purified bilirubin oxidase in Myrothecium verrucaria strain has potential application in dye effluent decolorization. Extracellular bilirubin oxidase decolorizes indigo carmine, biosorption and biodegradation of the dye is achieved with more than 98% decolorization efficiency after 7 days at 26°C. Additionally, the crude bilirubin oxidase can efficiently decolorize indigo carmine at 30°C to 50°C and pH 5.5-9.5 with dye concentrations of 50-200 mg/ml Albifimbria verrucaria

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.3.5 DTT 62.3% inhibition at 1 mM Albifimbria verrucaria
1.3.3.5 K+
-
 Albifimbria verrucaria
1.3.3.5 L-cysteine 87% inhibition at 1 mM, no significant effect at 0.1 mM Albifimbria verrucaria
1.3.3.5 Li+
-
 Albifimbria verrucaria
1.3.3.5 additional information the enzyme is not affected by urea and EDTA Albifimbria verrucaria
1.3.3.5 Na+
-
 Albifimbria verrucaria
1.3.3.5 NaN3 76% inhibition at 10 mM Albifimbria verrucaria

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.3.5 0.021
-
 bilirubin pH 7.5, 25°C Albifimbria verrucaria

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.3.3.5 extracellular
-
 Albifimbria verrucaria
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.3.3.5 Cu2+ highly activating, the enzyme contains four copper ions per functional unit Albifimbria verrucaria

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.3.5 68000
-
 about, native PAGE Albifimbria verrucaria
1.3.3.5 68100
-
 1 * 68100, SDS-PAGE Albifimbria verrucaria

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.3.5 2 bilirubin + O2 Albifimbria verrucaria
-
 2 biliverdin + 2 H2O
-
 ?
1.3.3.5 2 bilirubin + O2 Albifimbria verrucaria 3.2190
-
 2 biliverdin + 2 H2O
-
 ?
1.3.3.5 additional information Albifimbria verrucaria extracellular bilirubin oxidase decolorizes indigo carmine, biosorption and biodegradation of the dye is achved with more than 98% decolorization efficiency after 7 days at 26°C. Additionally, the crude bilirubin oxidase can efficiently decolorize indigo carmine at 30°C to 50°C and pH 5.5-9.5 with dye concentrations of 50-200 mg/ml, overview ?
-
 ?
1.3.3.5 additional information Albifimbria verrucaria 3.2190 extracellular bilirubin oxidase decolorizes indigo carmine, biosorption and biodegradation of the dye is achved with more than 98% decolorization efficiency after 7 days at 26°C. Additionally, the crude bilirubin oxidase can efficiently decolorize indigo carmine at 30°C to 50°C and pH 5.5-9.5 with dye concentrations of 50-200 mg/ml, overview ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.3.5 Albifimbria verrucaria
-
-
-
1.3.3.5 Albifimbria verrucaria 3.2190
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.3.5 extracellular enzyme 3.92fold by ammonium sulfate fractionation and anion exchange chromatography, an additional step of gel filtration lowers the enzyme purity and amount Albifimbria verrucaria

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.3.3.5 mycelium
-
 Albifimbria verrucaria
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.3.5 214.3
-
 purified enzyme after anion exchange chromatography step, pH 7.5, 25°C Albifimbria verrucaria

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.3.5 2 bilirubin + O2
-
 Albifimbria verrucaria 2 biliverdin + 2 H2O
-
 ?
1.3.3.5 2 bilirubin + O2
-
 Albifimbria verrucaria 3.2190 2 biliverdin + 2 H2O
-
 ?
1.3.3.5 additional information extracellular bilirubin oxidase decolorizes indigo carmine, biosorption and biodegradation of the dye is achved with more than 98% decolorization efficiency after 7 days at 26°C. Additionally, the crude bilirubin oxidase can efficiently decolorize indigo carmine at 30°C to 50°C and pH 5.5-9.5 with dye concentrations of 50-200 mg/ml, overview Albifimbria verrucaria ?
-
 ?
1.3.3.5 additional information the enzyme is also active with the laccase substrate 2, 2'-azino-bis (3-ethylbenzthiazoline-6-sulfonate) Albifimbria verrucaria ?
-
 ?
1.3.3.5 additional information extracellular bilirubin oxidase decolorizes indigo carmine, biosorption and biodegradation of the dye is achved with more than 98% decolorization efficiency after 7 days at 26°C. Additionally, the crude bilirubin oxidase can efficiently decolorize indigo carmine at 30°C to 50°C and pH 5.5-9.5 with dye concentrations of 50-200 mg/ml, overview Albifimbria verrucaria 3.2190 ?
-
 ?
1.3.3.5 additional information the enzyme is also active with the laccase substrate 2, 2'-azino-bis (3-ethylbenzthiazoline-6-sulfonate) Albifimbria verrucaria 3.2190 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.3.5 monomer 1 * 68100, SDS-PAGE Albifimbria verrucaria

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.3.5 40
-
 substrate bilirubin Albifimbria verrucaria

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.3.3.5 40 50 the enzyme activity is stable after incubation for 1 h at a temperature up to 40°C, thermal stability is decreased beyond 50?C Albifimbria verrucaria

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3.3.5 2.19
-
 bilirubin pH 7.5, 25°C Albifimbria verrucaria

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.3.5 7.5
-
 substrate bilirubin Albifimbria verrucaria

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.3.3.5 104.3
-
 bilirubin pH 7.5, 25°C Albifimbria verrucaria