Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Andreeva, I.G.; Golubeva, L.I.; Kuvaeva, T.M.; Gak, E.R.; Katashkina, J.I.; Mashko, S.V.
    Identification of Pantoea ananatis gene encoding membrane pyrroloquinoline quinone (PQQ)-dependent glucose dehydrogenase and pqqABCDEF operon essential for PQQ biosynthesis (2011), FEMS Microbiol. Lett., 318, 55-60.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.5.2 pyrroloquinoline quinone exogenously added cofector slightly activates the enzyme activity Pantoea ananatis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.5.2 operon qqqABCDEF, gene gcd Pantoea ananatis

Protein Variants

EC Number Protein Variants Comment Organism
1.1.5.2 additional information construction of DELTAgcd and DELTApqq mutants. qqqABCDEF is cloned in vivo and integrated into the chromosomes of Pantoea ananatis and Escherichia coli according to the Dual In/Out strategy. Introduction of a second copy of pqqABCDEF to Pantoea ananatis strain SC17(0) doubles the accumulation of PQQ. Integration of the operon into Escherichia coli strain MG1655DptsGDmanXY restores the growth of bacteria on glucose, overview Pantoea ananatis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.5.2 membrane
-
Pantoea ananatis 16020
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.5.2 D-glucose + ubiquinone Pantoea ananatis
-
D-glucono-1,5-lactone + ubiquinol
-
?
1.1.5.2 D-glucose + ubiquinone Pantoea ananatis SC17(0)
-
D-glucono-1,5-lactone + ubiquinol
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.1.5.2 Pantoea ananatis D4P700 gene gcd, encoded in the operon pqqABCDEF together with cofactor pyrroloquinoline quinone, PQQ
-
1.1.5.2 Pantoea ananatis SC17(0) D4P700 gene gcd, encoded in the operon pqqABCDEF together with cofactor pyrroloquinoline quinone, PQQ
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.5.2 0.00245
-
native enzyme in cells of strain SC17(0), pH 6.5, temperature not specified in the publication Pantoea ananatis
1.1.5.2 0.0197
-
native enzyme in cells of strain SC17(0) in presence of 0.01 mM pyrroloquinoline quinone, pH 6.5, temperature not specified in the publication Pantoea ananatis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.5.2 D-glucose + ubiquinone
-
Pantoea ananatis D-glucono-1,5-lactone + ubiquinol
-
?
1.1.5.2 D-glucose + ubiquinone assayed with the electron acceptors phenazine methosulfate and dichlorphenolindophenol Pantoea ananatis D-glucono-1,5-lactone + ubiquinol
-
?
1.1.5.2 D-glucose + ubiquinone
-
Pantoea ananatis SC17(0) D-glucono-1,5-lactone + ubiquinol
-
?
1.1.5.2 D-glucose + ubiquinone assayed with the electron acceptors phenazine methosulfate and dichlorphenolindophenol Pantoea ananatis SC17(0) D-glucono-1,5-lactone + ubiquinol
-
?

Synonyms

EC Number Synonyms Comment Organism
1.1.5.2 mGDH
-
Pantoea ananatis
1.1.5.2 PQQ-dependent glucose dehydrogenase
-
Pantoea ananatis
1.1.5.2 PQQ-dependent mGDH
-
Pantoea ananatis
1.1.5.2 quinone-dependent glucose dehydrogenase
-
Pantoea ananatis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.5.2 6.5
-
assay at Pantoea ananatis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.5.2 pyrroloquinoline quinone PQQ, essential cofactor for enzyme activity, essential role of pqqABCDEF in PQQ biosynthesis, overview Pantoea ananatis

General Information

EC Number General Information Comment Organism
1.1.5.2 physiological function essential role of pqqABCDEF in pyrroloquinoline quinone biosynthesis Pantoea ananatis