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Comparison of the ADP-ribosylating thermozyme from Sulfolobus solfataricus and the mesophilic poly(ADP-ribose) polymerases

Faraone Mennella, M.R.; Castellano, S.; De Luca, P.; Discenza, A.; Gambacorta, A.; Nicolaus, B.; Farina, B.; FEMS Microbiol. Lett. 192, 9-14 (2000)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.4.2.30
Mg2+
5 mM, 6.3fold activation
Saccharolobus solfataricus
2.4.2.30
Phenanthroline
0.1 mM, 1.1fold activation
Saccharolobus solfataricus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.4.2.30
3-aminobenzamide
10 mM, 47% inhibition; 10 mM, 47% inhibition
Saccharolobus solfataricus
2.4.2.30
additional information
phenanthroline has no or a slightly activating effect
Saccharolobus solfataricus
2.4.2.30
nicotinamide
10 mM, 14% inhibition; 10 mM, 14% inhibition
Saccharolobus solfataricus
2.4.2.30
Zn2+
0.5 mM, 47% inhibition; 0.5 mM, 50% inhibition
Saccharolobus solfataricus
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.4.2.30
Mg2+
0.1 mM, 4.1fold activation
Saccharolobus solfataricus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.4.2.30
46500
-
gel filtration; gel filtration
Saccharolobus solfataricus
2.4.2.30
50000
-
1 * 50000, SDS-PAGE
Saccharolobus solfataricus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.4.2.30
Saccharolobus solfataricus
B3EWG9
-
-
2.4.2.30
Saccharolobus solfataricus
-
i.e. DSM5833
-
2.4.2.30
Saccharolobus solfataricus MT-4 / DSM 5833
-
i.e. DSM5833
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.2.30
NAD+ + (ADP-D-ribosyl)n-acceptor
-
725034
Saccharolobus solfataricus
nicotinamide + (ADP-D-ribosyl)n+1-acceptor + H+
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
2.4.2.30
monomer
1 * 50000, SDS-PAGE
Saccharolobus solfataricus
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.4.2.30
80
-
assay at
Saccharolobus solfataricus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.4.2.30
8
-
assay at
Saccharolobus solfataricus
pI Value
EC Number
Organism
Commentary
pI Value Maximum
pI Value
2.4.2.30
Saccharolobus solfataricus
; isoelectric focusing
7.2
7
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.4.2.30
Mg2+
5 mM, 6.3fold activation
Saccharolobus solfataricus
2.4.2.30
Phenanthroline
0.1 mM, 1.1fold activation
Saccharolobus solfataricus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.4.2.30
3-aminobenzamide
10 mM, 47% inhibition
Saccharolobus solfataricus
2.4.2.30
additional information
phenanthroline has no or a slightly activating effect
Saccharolobus solfataricus
2.4.2.30
nicotinamide
10 mM, 14% inhibition
Saccharolobus solfataricus
2.4.2.30
Zn2+
0.5 mM, 47% inhibition
Saccharolobus solfataricus
2.4.2.30
Zn2+
0.5 mM, 50% inhibition
Saccharolobus solfataricus
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.4.2.30
Mg2+
0.1 mM, 4.1fold activation
Saccharolobus solfataricus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.4.2.30
46500
-
gel filtration
Saccharolobus solfataricus
2.4.2.30
50000
-
1 * 50000, SDS-PAGE
Saccharolobus solfataricus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.2.30
NAD+ + (ADP-D-ribosyl)n-acceptor
-
725034
Saccharolobus solfataricus
nicotinamide + (ADP-D-ribosyl)n+1-acceptor + H+
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.4.2.30
monomer
1 * 50000, SDS-PAGE
Saccharolobus solfataricus
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.4.2.30
80
-
assay at
Saccharolobus solfataricus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.4.2.30
8
-
assay at
Saccharolobus solfataricus
pI Value (protein specific)
EC Number
Organism
Commentary
pI Value Maximum
pI Value
2.4.2.30
Saccharolobus solfataricus
-
7.2
7
2.4.2.30
Saccharolobus solfataricus
isoelectric focusing
7.2
7