Literature summary extracted from
Derrer, B.; Windeisen, V.; Guedez Rodriguez, G.; Seidler, J.; Gengenbacher, M.; Lehmann, W.; Rippe, K.; Sinning, I.; Tews, I.; Kappes, B.
Defining the structural requirements for ribose 5-phosphate-binding and intersubunit cross-talk of the malarial pyridoxal 5-phosphate synthase (2010), FEBS Lett., 584, 4169-4174.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
4.3.3.6 |
His-tagged protein expressed in Escherichia coli BL21-CodonPlus(DE3)-RIL |
Plasmodium falciparum |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
4.3.3.6 |
DELTA 270-301 |
mainly monomer, Pdx2 activation, no pyridoxal 5'-phosphate synthesis |
Plasmodium falciparum |
4.3.3.6 |
DELTA 273-301 |
dodecamer, Pdx2 activation, no pyridoxal 5'-phosphate synthesis |
Plasmodium falciparum |
4.3.3.6 |
DELTA 279-301 |
dodecamer, Pdx2 activation, reduced pyridoxal 5'-phosphate synthesis |
Plasmodium falciparum |
4.3.3.6 |
DELTA 287-301 |
dodecamer, Pdx2 activation, precipitates upon addition of glyceraldehyd 3-phosphate |
Plasmodium falciparum |
4.3.3.6 |
DELTA 293-301 |
behaviour like DELTA 287-301 |
Plasmodium falciparum |
4.3.3.6 |
DELTA 295-301 |
behaviour like DELTA 287-301 |
Plasmodium falciparum |
4.3.3.6 |
S270A/DELTA 273-301 |
dodecamer |
Plasmodium falciparum |
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
4.3.3.6 |
29000 |
- |
12 * 29000, two interacting hexamers, analytical ultracentrifugation, 35320.7 Da determined by ESI-MS |
Plasmodium falciparum |
4.3.3.6 |
363000 |
- |
analytical ultracentrifugation, 420000 calculated |
Plasmodium falciparum |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
4.3.3.6 |
Plasmodium falciparum |
C6KT50 |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
4.3.3.6 |
immobilized metal ion affinity chromatography (Ni2+), gel filtration |
Plasmodium falciparum |
Specific Activity [micromol/min/mg]
EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Comment |
Organism |
---|
4.3.3.6 |
0.00025 |
- |
DELTA 279-301 protein, in the presence of Pdx2, pH 8.0, 37°C |
Plasmodium falciparum |
4.3.3.6 |
0.000779 |
- |
wild-type protein, in the presence of Pdx2, pH 8.0, 37°C |
Plasmodium falciparum |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
4.3.3.6 |
D-ribose 5-phosphate + glyceraldehyde 3-phosphate + L-glutamine |
in the presence of Pdx2 |
Plasmodium falciparum |
pyridoxal 5'-phosphate + L-glutamate + 3 H2O + phosphate |
- |
? |
|
4.3.3.6 |
D-ribose 5-phosphate + glyceraldehyde 3-phosphate + NH4+ |
in the absence of Pdx2 |
Plasmodium falciparum |
pyridoxal 5'-phosphate + 4 H2O + phosphate |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
4.3.3.6 |
dodecamer |
12 * 29000, two interacting hexamers, analytical ultracentrifugation, 35320.7 Da determined by ESI-MS |
Plasmodium falciparum |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
4.3.3.6 |
pyridoxal 5-phosphate synthase |
complex of Pdx1 and Pdx2 |
Plasmodium falciparum |