BRENDA - Enzyme Database

A unique biotin carboxyl carrier protein in archaeon Sulfolobus tokodaii

Li, Y.Q.; Sueda, S.; Kondo, H.; Kawarabayasi, Y.; FEBS Lett. 580, 1536-1540 (2006)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Cloned (Commentary)
Organism
6.3.4.15
overexpression in Escherichia coli
Escherichia coli
6.3.4.15
overexpression in Escherichia coli
Sulfurisphaera tokodaii
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
6.3.4.15
0.00058
-
[Sulfolobus tokodaii apo-biotin-carboxyl-carrier-protein]-lysine
pH 8.0, 70°C, wild type enzyme
Sulfurisphaera tokodaii
6.3.4.15
0.011
-
[Escherichia coli apo-biotin-carboxyl-carrier-protein]-L-lysine
pH 8.0, 37°C, wild type enzyme
Escherichia coli
6.3.4.15
0.03
-
[Sulfolobus tokodaii apo-biotin-carboxyl-carrier-protein mutant S136M]-lysine
pH 8.0, 37°C, wild type enzyme
Sulfurisphaera tokodaii
6.3.4.15
0.12
-
[Escherichia coli apo-biotin-carboxyl-carrier-protein mutant M136S]-L-lysine
pH 8.0, 37°C, wild type enzyme
Escherichia coli
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
6.3.4.15
26630
-
MALDI TOF mass spectrometry
Sulfurisphaera tokodaii
6.3.4.15
35310
-
MALDI TOF mass spectrometry
Escherichia coli
Organism
EC Number
Organism
UniProt
Commentary
Textmining
6.3.4.15
Escherichia coli
-
-
-
6.3.4.15
Sulfurisphaera tokodaii
F9VNG7
-
-
6.3.4.15
Sulfurisphaera tokodaii 7
F9VNG7
-
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
6.3.4.15
-
Escherichia coli
6.3.4.15
-
Sulfurisphaera tokodaii
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
6.3.4.15
ATP + biotin + [Escherichia coli apo-biotin-carboxyl-carrier-protein mutant M136S]-L-lysine
biotin carboxyl carrier protein (BCCP) is one subunit or domain of biotin-dependent enzymes. Biotin carboxyl carrier protein becomes an active substrate for carboxylation and carboxyl transfer, after biotinylation of its canonical lysine residue by biotin protein ligase (BPL). BCCP carries a characteristic local sequence surrounding the canonical lysine residue, typically -M-K-M-. Sulfolobus tokodaii is unique in that its biotin carboxyl carrier protein has serine replaced for the methionine C-terminal to the lysine. This biotin carboxyl carrier protein is biotinylated by its own biotin protein ligase, but not by Escherichia coli biotin protein ligase. Likewise, Escherichia coli biotin carboxyl carrier protein is not biotinylated by Sulfolobus tokodaii biotin protein ligase
724993
Escherichia coli
AMP + diphosphate + [Escherichia coli biotin-carboxyl-carrier-protein mutant M136S]-biotinyl-L-lysine
-
-
-
?
6.3.4.15
ATP + biotin + [Escherichia coli apo-biotin-carboxyl-carrier-protein]-L-lysine
biotin carboxyl carrier protein (BCCP) is one subunit or domain of biotin-dependent enzymes. Biotin carboxyl carrier protein becomes an active substrate for carboxylation and carboxyl transfer, after biotinylation of its canonical lysine residue by biotin protein ligase (BPL). BCCP carries a characteristic local sequence surrounding the canonical lysine residue, typically -M-K-M-. Sulfolobus tokodaii is unique in that its biotin carboxyl carrier protein has serine replaced for the methionine C-terminal to the lysine. This biotin carboxyl carrier protein is biotinylated by its own biotin protein ligase, but not by Escherichia coli biotin protein ligase. Likewise, Escherichia coli biotin carboxyl carrier protein is not biotinylated by Sulfolobus tokodaii biotin protein ligase
724993
Escherichia coli
AMP + diphosphate + [Escherichia coli biotin-carboxyl-carrier-protein]-biotinyl-L-lysine
-
-
-
?
6.3.4.15
ATP + biotin + [Sulfolobus tokodaii apo-biotin-carboxyl-carrier-protein mutant S136M]-lysine
biotin carboxyl carrier protein (BCCP) is one subunit or domain of biotin-dependent enzymes. BCCP becomes an active substrate for carboxylation and carboxyl transfer, after biotinylation of its canonical lysine residue by biotin protein ligase (BPL). BCCP carries a characteristic local sequence surrounding the canonical lysine residue, typically -M-K-M-. Sulfolobus tokodaii is unique in that its biotin carboxyl carrier protein has serine replaced for the methionine C-terminal to the lysine. This biotin carboxyl carrier protein is biotinylated by its own biotin protein ligase, but not by Escherichia coli biotin protein ligase. Likewise, Escherichia coli biotin carboxyl carrier protein is not biotinylated by Sulfolobus tokodaii biotin protein ligase
724993
Sulfurisphaera tokodaii
AMP + diphosphate + [Sulfolobus tokodaii biotin-carboxyl-carrier-protein mutant S136M]-biotinyllysine
-
-
-
?
6.3.4.15
ATP + biotin + [Sulfolobus tokodaii apo-biotin-carboxyl-carrier-protein mutant S136M]-lysine
biotin carboxyl carrier protein (BCCP) is one subunit or domain of biotin-dependent enzymes. BCCP becomes an active substrate for carboxylation and carboxyl transfer, after biotinylation of its canonical lysine residue by biotin protein ligase (BPL). BCCP carries a characteristic local sequence surrounding the canonical lysine residue, typically -M-K-M-. Sulfolobus tokodaii is unique in that its biotin carboxyl carrier protein has serine replaced for the methionine C-terminal to the lysine. This biotin carboxyl carrier protein is biotinylated by its own biotin protein ligase, but not by Escherichia coli biotin protein ligase. Likewise, Escherichia coli biotin carboxyl carrier protein is not biotinylated by Sulfolobus tokodaii biotin protein ligase
724993
Sulfurisphaera tokodaii 7
AMP + diphosphate + [Sulfolobus tokodaii biotin-carboxyl-carrier-protein mutant S136M]-biotinyllysine
-
-
-
?
6.3.4.15
ATP + biotin + [Sulfolobus tokodaii apo-biotin-carboxyl-carrier-protein]-lysine
biotin carboxyl carrier protein (BCCP) is one subunit or domain of biotin-dependent enzymes. BCCP becomes an active substrate for carboxylation and carboxyl transfer, after biotinylation of its canonical lysine residue by biotin protein ligase (BPL). BCCP carries a characteristic local sequence surrounding the canonical lysine residue, typically -M-K-M-. Sulfolobus tokodaii is unique in that its biotin carboxyl carrier protein has serine replaced for the methionine C-terminal to the lysine. This biotin carboxyl carrier protein is biotinylated by its own biotin protein ligase, but not by Escherichia coli biotin protein ligase. Likewise, Escherichia coli biotin carboxyl carrier protein is not biotinylated by Sulfolobus tokodaii biotin protein ligase
724993
Sulfurisphaera tokodaii
AMP + diphosphate + [Sulfolobus tokodaii biotin-carboxyl-carrier-protein]-biotinyllysine
-
-
-
?
6.3.4.15
ATP + biotin + [Sulfolobus tokodaii apo-biotin-carboxyl-carrier-protein]-lysine
biotin carboxyl carrier protein (BCCP) is one subunit or domain of biotin-dependent enzymes. BCCP becomes an active substrate for carboxylation and carboxyl transfer, after biotinylation of its canonical lysine residue by biotin protein ligase (BPL). BCCP carries a characteristic local sequence surrounding the canonical lysine residue, typically -M-K-M-. Sulfolobus tokodaii is unique in that its biotin carboxyl carrier protein has serine replaced for the methionine C-terminal to the lysine. This biotin carboxyl carrier protein is biotinylated by its own biotin protein ligase, but not by Escherichia coli biotin protein ligase. Likewise, Escherichia coli biotin carboxyl carrier protein is not biotinylated by Sulfolobus tokodaii biotin protein ligase
724993
Sulfurisphaera tokodaii 7
AMP + diphosphate + [Sulfolobus tokodaii biotin-carboxyl-carrier-protein]-biotinyllysine
-
-
-
?
Synonyms
EC Number
Synonyms
Commentary
Organism
6.3.4.15
STK_15250
locus name
Sulfurisphaera tokodaii
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
6.3.4.15
0.004
-
[Sulfolobus tokodaii apo-biotin-carboxyl-carrier-protein]-lysine
pH 8.0, 70°C, wild type enzyme
Sulfurisphaera tokodaii
6.3.4.15
0.0097
-
[Sulfolobus tokodaii apo-biotin-carboxyl-carrier-protein mutant S136M]-lysine
pH 8.0, 37°C, wild type enzyme
Sulfurisphaera tokodaii
6.3.4.15
0.165
-
[Escherichia coli apo-biotin-carboxyl-carrier-protein]-L-lysine
pH 8.0, 37°C, wild type enzyme
Escherichia coli
6.3.4.15
0.58
-
[Escherichia coli apo-biotin-carboxyl-carrier-protein mutant M136S]-L-lysine
pH 8.0, 37°C, wild type enzyme
Escherichia coli
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
6.3.4.15
overexpression in Escherichia coli
Escherichia coli
6.3.4.15
overexpression in Escherichia coli
Sulfurisphaera tokodaii
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
6.3.4.15
0.00058
-
[Sulfolobus tokodaii apo-biotin-carboxyl-carrier-protein]-lysine
pH 8.0, 70°C, wild type enzyme
Sulfurisphaera tokodaii
6.3.4.15
0.011
-
[Escherichia coli apo-biotin-carboxyl-carrier-protein]-L-lysine
pH 8.0, 37°C, wild type enzyme
Escherichia coli
6.3.4.15
0.03
-
[Sulfolobus tokodaii apo-biotin-carboxyl-carrier-protein mutant S136M]-lysine
pH 8.0, 37°C, wild type enzyme
Sulfurisphaera tokodaii
6.3.4.15
0.12
-
[Escherichia coli apo-biotin-carboxyl-carrier-protein mutant M136S]-L-lysine
pH 8.0, 37°C, wild type enzyme
Escherichia coli
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
6.3.4.15
26630
-
MALDI TOF mass spectrometry
Sulfurisphaera tokodaii
6.3.4.15
35310
-
MALDI TOF mass spectrometry
Escherichia coli
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
6.3.4.15
-
Escherichia coli
6.3.4.15
-
Sulfurisphaera tokodaii
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
6.3.4.15
ATP + biotin + [Escherichia coli apo-biotin-carboxyl-carrier-protein mutant M136S]-L-lysine
biotin carboxyl carrier protein (BCCP) is one subunit or domain of biotin-dependent enzymes. Biotin carboxyl carrier protein becomes an active substrate for carboxylation and carboxyl transfer, after biotinylation of its canonical lysine residue by biotin protein ligase (BPL). BCCP carries a characteristic local sequence surrounding the canonical lysine residue, typically -M-K-M-. Sulfolobus tokodaii is unique in that its biotin carboxyl carrier protein has serine replaced for the methionine C-terminal to the lysine. This biotin carboxyl carrier protein is biotinylated by its own biotin protein ligase, but not by Escherichia coli biotin protein ligase. Likewise, Escherichia coli biotin carboxyl carrier protein is not biotinylated by Sulfolobus tokodaii biotin protein ligase
724993
Escherichia coli
AMP + diphosphate + [Escherichia coli biotin-carboxyl-carrier-protein mutant M136S]-biotinyl-L-lysine
-
-
-
?
6.3.4.15
ATP + biotin + [Escherichia coli apo-biotin-carboxyl-carrier-protein]-L-lysine
biotin carboxyl carrier protein (BCCP) is one subunit or domain of biotin-dependent enzymes. Biotin carboxyl carrier protein becomes an active substrate for carboxylation and carboxyl transfer, after biotinylation of its canonical lysine residue by biotin protein ligase (BPL). BCCP carries a characteristic local sequence surrounding the canonical lysine residue, typically -M-K-M-. Sulfolobus tokodaii is unique in that its biotin carboxyl carrier protein has serine replaced for the methionine C-terminal to the lysine. This biotin carboxyl carrier protein is biotinylated by its own biotin protein ligase, but not by Escherichia coli biotin protein ligase. Likewise, Escherichia coli biotin carboxyl carrier protein is not biotinylated by Sulfolobus tokodaii biotin protein ligase
724993
Escherichia coli
AMP + diphosphate + [Escherichia coli biotin-carboxyl-carrier-protein]-biotinyl-L-lysine
-
-
-
?
6.3.4.15
ATP + biotin + [Sulfolobus tokodaii apo-biotin-carboxyl-carrier-protein mutant S136M]-lysine
biotin carboxyl carrier protein (BCCP) is one subunit or domain of biotin-dependent enzymes. BCCP becomes an active substrate for carboxylation and carboxyl transfer, after biotinylation of its canonical lysine residue by biotin protein ligase (BPL). BCCP carries a characteristic local sequence surrounding the canonical lysine residue, typically -M-K-M-. Sulfolobus tokodaii is unique in that its biotin carboxyl carrier protein has serine replaced for the methionine C-terminal to the lysine. This biotin carboxyl carrier protein is biotinylated by its own biotin protein ligase, but not by Escherichia coli biotin protein ligase. Likewise, Escherichia coli biotin carboxyl carrier protein is not biotinylated by Sulfolobus tokodaii biotin protein ligase
724993
Sulfurisphaera tokodaii
AMP + diphosphate + [Sulfolobus tokodaii biotin-carboxyl-carrier-protein mutant S136M]-biotinyllysine
-
-
-
?
6.3.4.15
ATP + biotin + [Sulfolobus tokodaii apo-biotin-carboxyl-carrier-protein mutant S136M]-lysine
biotin carboxyl carrier protein (BCCP) is one subunit or domain of biotin-dependent enzymes. BCCP becomes an active substrate for carboxylation and carboxyl transfer, after biotinylation of its canonical lysine residue by biotin protein ligase (BPL). BCCP carries a characteristic local sequence surrounding the canonical lysine residue, typically -M-K-M-. Sulfolobus tokodaii is unique in that its biotin carboxyl carrier protein has serine replaced for the methionine C-terminal to the lysine. This biotin carboxyl carrier protein is biotinylated by its own biotin protein ligase, but not by Escherichia coli biotin protein ligase. Likewise, Escherichia coli biotin carboxyl carrier protein is not biotinylated by Sulfolobus tokodaii biotin protein ligase
724993
Sulfurisphaera tokodaii 7
AMP + diphosphate + [Sulfolobus tokodaii biotin-carboxyl-carrier-protein mutant S136M]-biotinyllysine
-
-
-
?
6.3.4.15
ATP + biotin + [Sulfolobus tokodaii apo-biotin-carboxyl-carrier-protein]-lysine
biotin carboxyl carrier protein (BCCP) is one subunit or domain of biotin-dependent enzymes. BCCP becomes an active substrate for carboxylation and carboxyl transfer, after biotinylation of its canonical lysine residue by biotin protein ligase (BPL). BCCP carries a characteristic local sequence surrounding the canonical lysine residue, typically -M-K-M-. Sulfolobus tokodaii is unique in that its biotin carboxyl carrier protein has serine replaced for the methionine C-terminal to the lysine. This biotin carboxyl carrier protein is biotinylated by its own biotin protein ligase, but not by Escherichia coli biotin protein ligase. Likewise, Escherichia coli biotin carboxyl carrier protein is not biotinylated by Sulfolobus tokodaii biotin protein ligase
724993
Sulfurisphaera tokodaii
AMP + diphosphate + [Sulfolobus tokodaii biotin-carboxyl-carrier-protein]-biotinyllysine
-
-
-
?
6.3.4.15
ATP + biotin + [Sulfolobus tokodaii apo-biotin-carboxyl-carrier-protein]-lysine
biotin carboxyl carrier protein (BCCP) is one subunit or domain of biotin-dependent enzymes. BCCP becomes an active substrate for carboxylation and carboxyl transfer, after biotinylation of its canonical lysine residue by biotin protein ligase (BPL). BCCP carries a characteristic local sequence surrounding the canonical lysine residue, typically -M-K-M-. Sulfolobus tokodaii is unique in that its biotin carboxyl carrier protein has serine replaced for the methionine C-terminal to the lysine. This biotin carboxyl carrier protein is biotinylated by its own biotin protein ligase, but not by Escherichia coli biotin protein ligase. Likewise, Escherichia coli biotin carboxyl carrier protein is not biotinylated by Sulfolobus tokodaii biotin protein ligase
724993
Sulfurisphaera tokodaii 7
AMP + diphosphate + [Sulfolobus tokodaii biotin-carboxyl-carrier-protein]-biotinyllysine
-
-
-
?
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
6.3.4.15
0.004
-
[Sulfolobus tokodaii apo-biotin-carboxyl-carrier-protein]-lysine
pH 8.0, 70°C, wild type enzyme
Sulfurisphaera tokodaii
6.3.4.15
0.0097
-
[Sulfolobus tokodaii apo-biotin-carboxyl-carrier-protein mutant S136M]-lysine
pH 8.0, 37°C, wild type enzyme
Sulfurisphaera tokodaii
6.3.4.15
0.165
-
[Escherichia coli apo-biotin-carboxyl-carrier-protein]-L-lysine
pH 8.0, 37°C, wild type enzyme
Escherichia coli
6.3.4.15
0.58
-
[Escherichia coli apo-biotin-carboxyl-carrier-protein mutant M136S]-L-lysine
pH 8.0, 37°C, wild type enzyme
Escherichia coli
KCat/KM [mM/s]
EC Number
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
6.3.4.15
0.32
-
[Sulfolobus tokodaii apo-biotin-carboxyl-carrier-protein mutant S136M]-lysine
pH 8.0, 37°C, wild type enzyme
Sulfurisphaera tokodaii
6.3.4.15
0.49
-
[Escherichia coli apo-biotin-carboxyl-carrier-protein mutant M136S]-L-lysine
pH 8.0, 37°C, wild type enzyme
Escherichia coli
6.3.4.15
6.9
-
[Sulfolobus tokodaii apo-biotin-carboxyl-carrier-protein]-lysine
pH 8.0, 70°C, wild type enzyme
Sulfurisphaera tokodaii
6.3.4.15
15
-
[Escherichia coli apo-biotin-carboxyl-carrier-protein]-L-lysine
pH 8.0, 37°C, wild type enzyme
Escherichia coli
KCat/KM [mM/s] (protein specific)
EC Number
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
6.3.4.15
0.32
-
[Sulfolobus tokodaii apo-biotin-carboxyl-carrier-protein mutant S136M]-lysine
pH 8.0, 37°C, wild type enzyme
Sulfurisphaera tokodaii
6.3.4.15
0.49
-
[Escherichia coli apo-biotin-carboxyl-carrier-protein mutant M136S]-L-lysine
pH 8.0, 37°C, wild type enzyme
Escherichia coli
6.3.4.15
6.9
-
[Sulfolobus tokodaii apo-biotin-carboxyl-carrier-protein]-lysine
pH 8.0, 70°C, wild type enzyme
Sulfurisphaera tokodaii
6.3.4.15
15
-
[Escherichia coli apo-biotin-carboxyl-carrier-protein]-L-lysine
pH 8.0, 37°C, wild type enzyme
Escherichia coli