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Literature summary extracted from

  • Corbett, K.; Fordham-Skelton, A.P.; Gatehouse, J.A.; Davis, B.G.
    Tailoring the substrate specificity of the beta-glycosidase from the thermophilic archaeon Sulfolobus solfataricus (2001), FEBS Lett., 509, 355-360.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.B26 wild-type, mutant E432C, mutant W433C and mutant M439C enzymes are expressed in Escherichia coli as recombinant proteins containing an N-terminal His6-tag Saccharolobus solfataricus

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.B26 E432C kcat/KM is reduced for 4-methylumbelliferyl beta-D-glucopyranoside is reduced 200fold, kcat/KM is reduced for 4-methylumbelliferyl beta-D-galactopyranoside is reduced 130fold, kcat/KM is reduced for 4-methylumbelliferyl beta-D-mannopyranoside is reduced 16fold, kcat/KM is reduced for 4-methylumbelliferyl beta-D-xylopyranoside is reduced 14fold Saccharolobus solfataricus
3.2.1.B26 M439C shows almost identical values to wild-type for 4-methylumbelliferyl beta-D-glucopyranoside, 4-methylumbelliferyl beta-D-galactopyranoside or 4-methylumbelliferyl beta-D-mannopyranoside substrates. kcat/KM for 4-methylumbelliferyl beta-D-fucopyranoside is 1.8-fold lower than wild-type value and kcat/KM for 4-methylumbelliferyl beta-D-xylopyranoside is 4.7fold higher than wild-type Saccharolobus solfataricus
3.2.1.B26 W433C kcat/KM is reduced for 4-methylumbelliferyl beta-D-glucopyranoside is reduced 140fold, kcat/KM is reduced for 4-methylumbelliferyl beta-D-galactopyranoside is reduced 230fold, kcat/KM is reduced for 4-methylumbelliferyl beta-D-mannopyranoside is reduced 10fold, kcat/KM is reduced for 4-methylumbelliferyl beta-D-xylopyranoside is reduced 12fold Saccharolobus solfataricus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.B26 0.011
-
 4-methylumbelliferyl beta-D-fucopyranoside pH 6.5, 80°C, wild-type enzyme Saccharolobus solfataricus
3.2.1.B26 0.023
-
 4-methylumbelliferyl beta-D-fucopyranoside pH 6.5, 80°C, mutant enzyme M439C Saccharolobus solfataricus
3.2.1.B26 0.036
-
 4-methylumbelliferyl beta-D-mannopyranoside pH 6.5, 80°C, wild-type enzyme Saccharolobus solfataricus
3.2.1.B26 0.042
-
 4-methylumbelliferyl beta-D-mannopyranoside pH 6.5, 80°C, mutant enzyme M439C Saccharolobus solfataricus
3.2.1.B26 0.046
-
 4-methylumbelliferyl beta-D-glucopyranoside pH 6.5, 80°C, wild-type enzyme Saccharolobus solfataricus
3.2.1.B26 0.066
-
 4-methylumbelliferyl beta-D-galactopyranoside pH 6.5, 80°C, wild-type enzyme Saccharolobus solfataricus
3.2.1.B26 0.068
-
 4-methylumbelliferyl beta-D-glucopyranoside pH 6.5, 80°C, mutant enzyme M439C Saccharolobus solfataricus
3.2.1.B26 0.068
-
 4-methylumbelliferyl beta-D-xylopyranoside pH 6.5, 80°C, mutant enzyme M439C Saccharolobus solfataricus
3.2.1.B26 0.083
-
 4-methylumbelliferyl beta-D-galactopyranoside pH 6.5, 80°C, mutant enzyme M439C Saccharolobus solfataricus
3.2.1.B26 0.13
-
 4-methylumbelliferyl beta-D-xylopyranoside pH 6.5, 80°C, wild-type enzyme Saccharolobus solfataricus
3.2.1.B26 0.18
-
 4-methylumbelliferyl beta-D-mannopyranoside pH 6.5, 80°C, mutant enzyme W433C Saccharolobus solfataricus
3.2.1.B26 0.34
-
 4-methylumbelliferyl beta-D-glucopyranoside pH 6.5, 80°C, mutant enzyme E432C Saccharolobus solfataricus
3.2.1.B26 0.34
-
 4-methylumbelliferyl beta-D-fucopyranoside pH 6.5, 80°C, mutant enzyme E432C Saccharolobus solfataricus
3.2.1.B26 0.41
-
 4-methylumbelliferyl beta-D-fucopyranoside pH 6.5, 80°C, mutant enzyme W433C Saccharolobus solfataricus
3.2.1.B26 0.47
-
 4-methylumbelliferyl beta-D-galactopyranoside pH 6.5, 80°C, mutant enzyme E432C Saccharolobus solfataricus
3.2.1.B26 0.59
-
 4-methylumbelliferyl beta-D-xylopyranoside pH 6.5, 80°C, mutant enzyme W433C Saccharolobus solfataricus
3.2.1.B26 0.9
-
 4-methylumbelliferyl beta-D-mannopyranoside pH 6.5, 80°C, mutant enzyme E432C Saccharolobus solfataricus
3.2.1.B26 1.26
-
 4-methylumbelliferyl beta-D-xylopyranoside pH 6.5, 80°C, mutant enzyme E432C Saccharolobus solfataricus
3.2.1.B26 1.3
-
 4-methylumbelliferyl beta-D-glucuronic acid pH 6.5, 80°C, wild-type enzyme Saccharolobus solfataricus
3.2.1.B26 1.4
-
 4-methylumbelliferyl beta-D-glucuronic acid pH 6.5, 80°C, mutant enzyme M439C Saccharolobus solfataricus
3.2.1.B26 1.61
-
 4-methylumbelliferyl beta-D-glucopyranoside pH 6.5, 80°C, mutant enzyme W433C Saccharolobus solfataricus
3.2.1.B26 2.2
-
 4-methylumbelliferyl beta-D-galactopyranoside pH 6.5, 80°C, mutant enzyme W433C Saccharolobus solfataricus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.B26 57000
-
 2 * 57000, SDS-PAGE Saccharolobus solfataricus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.B26 Saccharolobus solfataricus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.B26 His6-tagged mutant enzyme Saccharolobus solfataricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.B26 4-methylumbelliferyl beta-D-fucopyranoside + H2O
-
 Saccharolobus solfataricus 4-methylumbelliferone + beta-D-fucopyranose
-
 ?
3.2.1.B26 4-methylumbelliferyl beta-D-galactopyranoside + H2O
-
 Saccharolobus solfataricus 4-methylumbelliferone + beta-D-galactopyranose
-
 ?
3.2.1.B26 4-methylumbelliferyl beta-D-glucopyranoside + H2O
-
 Saccharolobus solfataricus 4-methylumbelliferone + beta-D-glucopyranose
-
 ?
3.2.1.B26 4-methylumbelliferyl beta-D-glucuronic acid + H2O
-
 Saccharolobus solfataricus 4-methylumbelliferone + beta-D-glucuronic acid
-
 ?
3.2.1.B26 4-methylumbelliferyl beta-D-mannopyranoside + H2O
-
 Saccharolobus solfataricus 4-methylumbelliferone + beta-D-mannopyranose
-
 ?
3.2.1.B26 4-methylumbelliferyl beta-D-xylopyranoside + H2O
-
 Saccharolobus solfataricus 4-methylumbelliferone + beta-D-xylopyranose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.B26 dimer 2 * 57000, SDS-PAGE Saccharolobus solfataricus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.B26 LACS
-
 Saccharolobus solfataricus
3.2.1.B26 SsbetaG
-
 Saccharolobus solfataricus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.B26 80
-
 assay at Saccharolobus solfataricus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.B26 0.81
-
 4-methylumbelliferyl beta-D-glucuronic acid pH 6.5, 80°C, wild-type enzyme Saccharolobus solfataricus
3.2.1.B26 0.92
-
 4-methylumbelliferyl beta-D-mannopyranoside pH 6.5, 80°C, mutant enzyme W433C Saccharolobus solfataricus
3.2.1.B26 1.3
-
 4-methylumbelliferyl beta-D-glucuronic acid pH 6.5, 80°C, mutant enzyme M439C Saccharolobus solfataricus
3.2.1.B26 1.5
-
 4-methylumbelliferyl beta-D-xylopyranoside pH 6.5, 80°C, mutant enzyme W433C Saccharolobus solfataricus
3.2.1.B26 1.8
-
 4-methylumbelliferyl beta-D-mannopyranoside pH 6.5, 80°C, wild-type enzyme Saccharolobus solfataricus
3.2.1.B26 2.3
-
 4-methylumbelliferyl beta-D-mannopyranoside pH 6.5, 80°C, mutant enzyme M439C Saccharolobus solfataricus
3.2.1.B26 2.8
-
 4-methylumbelliferyl beta-D-mannopyranoside pH 6.5, 80°C, mutant enzyme E432C Saccharolobus solfataricus
3.2.1.B26 2.8
-
 4-methylumbelliferyl beta-D-xylopyranoside pH 6.5, 80°C, mutant enzyme E432C Saccharolobus solfataricus
3.2.1.B26 3.8
-
 4-methylumbelliferyl beta-D-xylopyranoside pH 6.5, 80°C, wild-type enzyme Saccharolobus solfataricus
3.2.1.B26 5.1
-
 4-methylumbelliferyl beta-D-glucopyranoside pH 6.5, 80°C, mutant enzyme E432C Saccharolobus solfataricus
3.2.1.B26 5.4
-
 4-methylumbelliferyl beta-D-galactopyranoside pH 6.5, 80°C, mutant enzyme E432C Saccharolobus solfataricus
3.2.1.B26 9.3
-
 4-methylumbelliferyl beta-D-xylopyranoside pH 6.5, 80°C, mutant enzyme M439C Saccharolobus solfataricus
3.2.1.B26 14
-
 4-methylumbelliferyl beta-D-galactopyranoside pH 6.5, 80°C, mutant enzyme W433C Saccharolobus solfataricus
3.2.1.B26 18
-
 4-methylumbelliferyl beta-D-fucopyranoside pH 6.5, 80°C, mutant enzyme E432C Saccharolobus solfataricus
3.2.1.B26 31
-
 4-methylumbelliferyl beta-D-fucopyranoside pH 6.5, 80°C, mutant enzyme W433C Saccharolobus solfataricus
3.2.1.B26 33
-
 4-methylumbelliferyl beta-D-glucopyranoside pH 6.5, 80°C, mutant enzyme W433C Saccharolobus solfataricus
3.2.1.B26 80
-
 4-methylumbelliferyl beta-D-fucopyranoside pH 6.5, 80°C, wild-type enzyme Saccharolobus solfataricus
3.2.1.B26 91
-
 4-methylumbelliferyl beta-D-fucopyranoside pH 6.5, 80°C, mutant enzyme M439C Saccharolobus solfataricus
3.2.1.B26 94
-
 4-methylumbelliferyl beta-D-galactopyranoside pH 6.5, 80°C, mutant enzyme M439C Saccharolobus solfataricus
3.2.1.B26 98
-
 4-methylumbelliferyl beta-D-galactopyranoside pH 6.5, 80°C, wild-type enzyme Saccharolobus solfataricus
3.2.1.B26 140
-
 4-methylumbelliferyl beta-D-glucopyranoside pH 6.5, 80°C, wild-type enzyme Saccharolobus solfataricus
3.2.1.B26 190
-
 4-methylumbelliferyl beta-D-glucopyranoside pH 6.5, 80°C, mutant enzyme M439C Saccharolobus solfataricus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.B26 6.5
-
 assay at Saccharolobus solfataricus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.B26 0.6
-
 4-methylumbelliferyl beta-D-glucuronic acid pH 6.5, 80°C, wild-type enzyme Saccharolobus solfataricus
3.2.1.B26 0.92
-
 4-methylumbelliferyl beta-D-glucuronic acid pH 6.5, 80°C, mutant enzyme M439C Saccharolobus solfataricus
3.2.1.B26 2.2
-
 4-methylumbelliferyl beta-D-xylopyranoside pH 6.5, 80°C, mutant enzyme E432C Saccharolobus solfataricus
3.2.1.B26 2.5
-
 4-methylumbelliferyl beta-D-xylopyranoside pH 6.5, 80°C, mutant enzyme W433C Saccharolobus solfataricus
3.2.1.B26 3.2
-
 4-methylumbelliferyl beta-D-mannopyranoside pH 6.5, 80°C, mutant enzyme E432C Saccharolobus solfataricus
3.2.1.B26 5.1
-
 4-methylumbelliferyl beta-D-mannopyranoside pH 6.5, 80°C, mutant enzyme W433C Saccharolobus solfataricus
3.2.1.B26 6.3
-
 4-methylumbelliferyl beta-D-galactopyranoside pH 6.5, 80°C, mutant enzyme W433C Saccharolobus solfataricus
3.2.1.B26 11
-
 4-methylumbelliferyl beta-D-galactopyranoside pH 6.5, 80°C, mutant enzyme E432C Saccharolobus solfataricus
3.2.1.B26 15
-
 4-methylumbelliferyl beta-D-glucopyranoside pH 6.5, 80°C, mutant enzyme E432C Saccharolobus solfataricus
3.2.1.B26 20
-
 4-methylumbelliferyl beta-D-glucopyranoside pH 6.5, 80°C, mutant enzyme W433C Saccharolobus solfataricus
3.2.1.B26 30
-
 4-methylumbelliferyl beta-D-xylopyranoside pH 6.5, 80°C, wild-type enzyme Saccharolobus solfataricus
3.2.1.B26 50
-
 4-methylumbelliferyl beta-D-mannopyranoside pH 6.5, 80°C, wild-type enzyme Saccharolobus solfataricus
3.2.1.B26 53
-
 4-methylumbelliferyl beta-D-fucopyranoside pH 6.5, 80°C, mutant enzyme E432C Saccharolobus solfataricus
3.2.1.B26 53
-
 4-methylumbelliferyl beta-D-mannopyranoside pH 6.5, 80°C, mutant enzyme M439C Saccharolobus solfataricus
3.2.1.B26 76
-
 4-methylumbelliferyl beta-D-fucopyranoside pH 6.5, 80°C, mutant enzyme W433C Saccharolobus solfataricus
3.2.1.B26 136
-
 4-methylumbelliferyl beta-D-xylopyranoside pH 6.5, 80°C, mutant enzyme M439C Saccharolobus solfataricus
3.2.1.B26 1130
-
 4-methylumbelliferyl beta-D-galactopyranoside pH 6.5, 80°C, mutant enzyme M439C Saccharolobus solfataricus
3.2.1.B26 1490
-
 4-methylumbelliferyl beta-D-galactopyranoside pH 6.5, 80°C, wild-type enzyme Saccharolobus solfataricus
3.2.1.B26 2900
-
 4-methylumbelliferyl beta-D-glucopyranoside pH 6.5, 80°C, mutant enzyme M439C Saccharolobus solfataricus
3.2.1.B26 2900
-
 4-methylumbelliferyl beta-D-glucopyranoside pH 6.5, 80°C, wild-type enzyme Saccharolobus solfataricus
3.2.1.B26 4000
-
 4-methylumbelliferyl beta-D-fucopyranoside pH 6.5, 80°C, mutant enzyme M439C Saccharolobus solfataricus
3.2.1.B26 7300
-
 4-methylumbelliferyl beta-D-fucopyranoside pH 6.5, 80°C, wild-type enzyme Saccharolobus solfataricus